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Plant endophyte for efficiently degrading phthalic acid ester and application of plant endophyte

A phthalate, high-efficiency technology, applied in the field of microorganisms, can solve problems such as environmental protection significance yet to be studied in depth, and achieve the effect of great application prospects

Inactive Publication Date: 2017-09-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have found that plant endophytes have the function of degrading environmental pollutants, but their ability to remove environmental pollutants, or their environmental significance, remains to be further studied

Method used

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  • Plant endophyte for efficiently degrading phthalic acid ester and application of plant endophyte
  • Plant endophyte for efficiently degrading phthalic acid ester and application of plant endophyte
  • Plant endophyte for efficiently degrading phthalic acid ester and application of plant endophyte

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041]Example 1 Isolation and Identification of Bacillus subtilis Enb-N Strain

[0042] 1. Isolation and screening of bacterial strains

[0043] The strain was isolated, domesticated and screened from a plant sample by a polluted canal in Shaoguan. The specific method includes the following steps:

[0044] (1) Several types of plant samples were collected from the surroundings of polluted water canals for the extraction of endophytic bacteria from cultivable plants.

[0045] (2) Take the plant sample back and wash it, rinse it with distilled water and drain the water droplets, cut the aboveground and underground parts of the plant sample separately, and cut them into thin sections, soak them in alcohol with a concentration of 75% by volume for 1 min, and then move them into the Aseptic operation table, continue to disinfect the plant surface, transfer the soaked sample to a new sterilized petri dish, pour in a mass concentration of 5% sodium hypochlorite solution and 30% hydr...

Embodiment 2

[0059] The growth curve determination of embodiment 2 bacillus subtilis Enb-N bacterial strain

[0060] Due to the concentration and optical density (OD) of the bacterial suspension 600 value) is directly proportional, so the concentration of the bacterial solution can be deduced by using a spectrophotometer to measure the optical density of the bacterial suspension, that is, the turbidimetric method is used to measure, and the measured OD 600 The growth curve of the bacteria under certain conditions can be drawn by plotting the value and the corresponding culture time.

[0061] (1) Select 25 mL of LB liquid culture medium, inoculate the bacterial liquid cultured by the bacteria of the present invention, and culture 0, 1, 3, 5, 7, 9, 11, 13, 15, 17, 20, 24, 26, 32h, use a spectrophotometer to measure the photometric value at a wavelength of 600nm, which is OD 600 value to draw a growth curve.

[0062] (2) The growth curve of the strain is as follows: figure 2 As shown, it...

Embodiment 3

[0063] Example 3 The biological characteristics of Bacillus subtilis Enb-N strain

[0064] 1. Experimental studies have shown that the Enb-N strain has the ability to secrete indole acetic acid, which can promote the growth and development of plants parasitized by plant endophytes.

[0065] At the same time, the strain also has the ability to decompose potassium and phosphorus; therefore, it can convert some mineral P and K in the soil into free P and K, which is beneficial to the growth and reproduction of microorganisms.

[0066] 2. Experimental studies have shown that the Enb-N strain has certain resistance to several common antibiotics, among which the resistance to rifampicin is stronger and the resistance to gentamicin is weaker; when chloramphenicol reaches 8ppm, When gentamicin reaches 0.5ppm, the growth can be completely inhibited; while tetracycline hydrochloride has a more obvious effect on the strain at 16ppm, and rifampicin begins to affect the strain at 16ppm.

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Abstract

The invention discloses plant endophyte for efficiently degrading phthalic acid ester and an application of the plant endophyte. The plant endophyte is a Bacillus subtilis Enb-N strain, the strain is collected in a Guangdong Microbiological Culture Collection Center on May 4, 2017, and the collection number of the strain is GDMCC NO: 60173. The strain can use phthalate as a sole carbon source and energy for growth and propagation, the degrading rate of DBP (dibutyl phthalate) can reach 95% or more, the strain can be used for treating phthalate pollutants, further has the functions of secreting indoleacetic acid and degrading potassium and phosphorus and has a certain resistance for common antibiotics, growth of the strain has strong resistance to Cd and Hg ions, and the strain has strong environmental adaptability and large application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. More specifically, it relates to a plant endophyte that efficiently degrades phthalates and its application. Background technique [0002] Phthalic acid esters (Phthalic Acid Esters, PAEs), also known as phthalates, are widely used in daily life and industry. Phthalic acid esters are defined as a class of chemical substances in organic chemistry, containing multiple isomers , of which dibutylphthalate (Dibutylphthalate, DBP) is widely used. PAEs are generally oily, slightly soluble in water, and easily soluble in organic solvents. Their environmental hazards are mainly teratogenic, carcinogenic, and mutagenic to humans and other animals. In addition, they are also harmful to lower organisms such as algae. Has toxicological effects, while DBP has acute or chronic toxicity in the aquatic environment. As a plasticizer, PAEs are widely used in daily life and industry with plastic products a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34B09C1/10C12R1/125A62D101/28C02F101/34
CPCA62D3/02A62D2101/28B09C1/10C02F3/34C02F2101/34C12N1/20C12N1/205C12R2001/125
Inventor 吕辉雄黄裕宏黄雪晶陈少华李军辉李智涛郑丽
Owner SOUTH CHINA AGRI UNIV
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