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Method and system for preventing and treating cryptocryon irritan diseases of aquaculture animals by hydroxyl radicals

A technology for stimulating Cryptocaryonium and aquaculture, applied in chemical instruments and methods, fish farming, animal husbandry wastewater treatment, etc. Complete recovery, improved effect

Active Publication Date: 2017-08-22
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical immersion includes freshwater immersion, rotational cultivation, and the use of ultraviolet light and ozone to treat aquaculture water, etc. , the effect is not obvious, and the operation process is cumbersome or the fish are particularly stressed, so it is not suitable for application in large-scale breeding plants
Chemical agents such as copper sulfate, methylene blue, malachite green, quinine hydrochloride, phenoxyethanol, etc. :349-361), although it has a certain inhibitory effect on stimulating Cryptocaryoniasis, long-term overdose will cause consequences such as drug residues and environmental pollution, and insecticides such as malachite green and methylene blue have been listed as prohibited aquatic products. use
The extremely serious abuse of drugs in domestic mariculture has caused many adverse consequences, such as the development of drug resistance; the destruction of the colony structure of beneficial organisms; the decline of self-immunity and even the induction of more serious diseases

Method used

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  • Method and system for preventing and treating cryptocryon irritan diseases of aquaculture animals by hydroxyl radicals
  • Method and system for preventing and treating cryptocryon irritan diseases of aquaculture animals by hydroxyl radicals
  • Method and system for preventing and treating cryptocryon irritan diseases of aquaculture animals by hydroxyl radicals

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Experimental program
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Effect test

Embodiment 1

[0063] In mid-October, the water temperature in the breeding pond was 22-25°C, the salinity was 23ppt, the pH was 7.36, the ammonia nitrogen was 0.037mg / L, the nitrite was 0.287mg / L, and the dissolved oxygen was 8.37mg / L. Red sea bream fry appeared in the breeding pond Decreased food intake, outlier, like to stay at the water inlet and outlet with mouth open, often rubbing the pool wall or bottom with the body, rapid breathing rate, and many white spots on the body surface. Through microscopic examination, it is found that the body surface skin, gills and fins secrete mucus to wrap the worm body, the color is black, and the shape is round or nucleated, which can be diagnosed as Cryptocaryon irritant infection. Wherein the culture pond (control group) is not treated, and a large number of deaths begin to occur after 2 days, and the mortality rate is more than 90% after 5 days. The other three culture ponds are respectively injected with seawater containing TRO doses, and the dos...

Embodiment 2

[0065] Characterize the killing conditions of Cryptocaryon larvae and trophozoites stimulated by different OH doses. Firstly, the morphological changes of Cryptocaryon larvae and trophozoites before and after OH killing were observed under microscope. The results are as follows: figure 2 and 3 shown (in figure 2 Among them, A is the bright-field photo of the stimulated Cryptocaryon larvae before treatment, B and C are the bright-field photos of the stimulated Cryptocaryon larvae after treatment; image 3 Among them, A is the bright-field photo of the stimulated Cryptocaryon trophozoite before treatment, B and C are the bright-field photos of the stimulated Cryptocaryon trophozoite after treatment). From figure 2 It can be seen that after the OH treatment, the larvae first slowed down their swimming speed, and then stayed in a small range and moved slowly. Then, the larvae vibrated obviously and rotated around their own long axis. Several vacuoles, protruding from the su...

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Abstract

The invention discloses a method and system for preventing and treating cryptocryon irritan diseases of aquaculture animals by hydroxyl radicals. Water outlets of aquaculture pools of the system are connected with a water inlet of a liquid flowmeter; a water outlet of the liquid flowmeter is connected with a water inlet of a liquid pump; a water outlet of the liquid pump is connected with a water inlet of a sieve bend; a water outlet of the sieve bend is connected with a water inlet of a protein separator; a water outlet of the protein separator is connected with a water inlet of a biological filter; a water outlet of the biological filter is connected with a water inlet of a buffer pool; a water outlet of the buffer pool is connected with a water inlet of a water quality detector; a water outlet of the water quality detector is divided into two ways, and respectively connected with a main water inlet of a venturi ejector and a water inlet of a liquid flowmeter; a water outlet of the liquid flowmeter is connected with a water inlet of a hydroxyl radical generation device; a water outlet of the hydroxyl radical generation device is connected with a side water inlet of the venturi ejector; a water outlet of the venturi ejector is connected with a water inlet of a liquid-liquid mixing and dissolving tank; a water outlet of the liquid-liquid mixing and dissolving tank is connected with a water inlet of a gas-liquid separator, and connected with a water inlet of a water quality detector.

Description

technical field [0001] The invention relates to the fields of plasma chemistry, aquaculture technology, marine environment engineering and the like, in particular to a method and a system for preventing and controlling cryptocaryon stimulant diseases of aquaculture animals by hydroxyl radicals. Background technique [0002] Cryptocaryon stimuli is a ciliated unicellular protozoan, mainly distributed in tropical and subtropical seas, and its life cycle can be divided into "trophoblast", "cyst precursor", "cyst" and "larvae" 4 stages, it can infect most sea bony fishes, has no strict selectivity to fish species and growth stages, is widely distributed, and easily causes a large number of deaths, and is extremely harmful to the mariculture industry (but Xueming. Stimulation Cryptocaryon irritans (Cryptocaryon irritans) passage, preservation and stimulation of Cryptocaryon disease control research [D]. Jinan University, 2006). [0003] At present, the aquaculture industry often...

Claims

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Application Information

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IPC IPC(8): A01K61/13A01K63/04C02F9/14C02F103/20
CPCA01K61/00A01K63/04A01K63/042A01K63/045A01K63/047C02F1/001C02F1/24C02F1/68C02F3/104C02F9/00C02F2103/20Y02A40/81
Inventor 白敏冬钟子清张芝涛李海燕
Owner XIAMEN UNIV
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