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Method for assessing risk of endosulfan in cancer promoting

An endosulfan and risk technology, which is applied in the fields of biochemical equipment and methods, microbial determination/inspection, instruments, etc., can solve the problems such as the impact of the accuracy of the results, the limitation of the number of studies, etc., and achieves a simple method, high correlation, and rapid evaluation. Effect

Inactive Publication Date: 2017-08-18
DALIAN MARITIME UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the studies in the NextBio database are clinical studies, and the results are relatively similar to the clinical situation. However, due to the limitation of the number of studies, the accuracy of the analyzed results is affected.
The Metacore database has more functional analysis, the results are reliable and accurate, and it is good at analyzing the transcription factor regulatory network, but it can only realize gene chip analysis of three species: human, rat, and mouse

Method used

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  • Method for assessing risk of endosulfan in cancer promoting
  • Method for assessing risk of endosulfan in cancer promoting
  • Method for assessing risk of endosulfan in cancer promoting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Endosulfan causes changes in differentially expressed genes in vascular endothelial cells:

[0028] Endosulfan exposure treatment was performed on vascular endothelial cells (HUVEC-C), and the experiment was divided into two groups: DMSO control group (dimethyl sulfoxide, D) and endosulfan experimental group (endosulfan, ES). The concentration of DMSO in the control group was 0.1%, the exposure concentration of endosulfan was 20 μM, and the exposure time was 48 hours. The cells were collected to extract total RNA for gene chip [Agilent Human Gene Expression Profiling, Shanghai Kangcheng Biological Co., Ltd., Whole Human Genome Oligo Microarray (4x44K, Agilent Technologies )] analysis, through gene expression profiling analysis, it was found that compared with the control group, 457 genes were down-regulated and 319 genes were up-regulated when exposed to endosulfan.

[0029] (2) Predictive analysis of human diseases caused by endosulfan exposure:

[0030] For the d...

Embodiment 2

[0034] (1) Gene expression profiling analysis of human prostate cancer:

[0035] In order to study the correlation between endosulfan and prostate cancer, a study on the gene expression profile of prostate cancer was screened out in the NextBio database according to the research species of human, the data type of gene chip data, and the common differential gene correlation P value less than 0.05. Clinical research data (from Stanford Microarray Database, GSE3933; URL http: / / genome-www5.stanford.edu / stanford university, document screenshots such as image 3 ). The data is provided by the Stanford University database, which contains the gene expression profiles of 62 prostate cancer tissues, 41 normal prostate tissues and 9 lymph node metastatic cancers. About 26,000 genes were detected in the whole study, and the prostate cancer tissues were compared with the normal ones. Compared with prostate tissue, there were 7767 genes with differential expression changes.

[0036] (2...

Embodiment 3

[0043] Endosulfan Exposure Causes Changes in PRL-3 Expression Levels:

[0044] Prostate cancer PC3 cells were exposed to endosulfan, and the experiment was divided into two groups: DMSO control group (dimethyl sulfoxide, D) and endosulfan experimental group (endosulfan, ES). The concentration of DMSO in the control group was 0.1%, the exposure concentration of endosulfan was 20 μM, and the exposure time was 48 hours. The cells in the DMSO group and the endosulfan experimental group were collected, and the total RNA was extracted by Trizol method, and the RNA concentration was determined and RNA quality inspection was carried out. The SYBR green (Applied Biosystems, ABI) method was used to carry out qRT-PCR reaction, and the primers (PRL-3 and GAPDH, see Table 2) were designed, GAPDH was used as an internal reference, and 2 -ΔΔCt Methods The differences in mRNA expression were expressed by the multiple of treated samples relative to untreated samples. The result is as Figure...

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Abstract

The invention provides a method for assessing risk of endosulfan in cancer promoting. The method includes the step of detecting expression of a biomarker of biological samples; the biomaker refers to PRL-3. The method is easy and convenient, high in relevance, and capable of assessing risk of endosulfan in cancer promoting rapidly. According to the method, PRL-3, screened by the bioinformatics analysis, is the key gene of the relevance between endosulfan and prostatic cancer; As proved in terms of the cytology, endosulfan has effect of improving migration capacity of prostatic cancer cells by improving the expression of PRL-3, that is, endosulfan has risk of promoting prostatic cancer. The method provides a significant reference value for searching the key gene causing human diseases from environmental pollutants, and also provides important clue for targeted therapy on the human diseases caused by environmental pollutants in the future.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for promoting cancer risk by environmental pollutants, in particular to a method for evaluating prostate cancer caused by endosulfan exposure. Background technique [0002] With the advancement of science and technology, the improvement of the level of industrialization, various chemical agents are constantly entering people's lives, among which the environmental hazards of persistent organic pollutants (POPs) and the impact on human health cannot be ignored, including two British (PCDD / Fs), polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), organochlorine pesticide endosulfan, etc. [0003] Endosulfan is an organochlorine pesticide, which is widely used in agricultural production as an insecticide. It has the characteristics of high biological toxicity, potential carcinogenicity and environmental estrogen effect. has attracted widespread attention in ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q1/6886C12Q2600/142C12Q2600/158G01N33/57434G01N2800/342
Inventor 徐丹郭瑜冰林丽梅孙野青
Owner DALIAN MARITIME UNIVERSITY
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