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Lactobacillus plantarum strain capable of producing extracellular polysaccharide

A technology of Lactobacillus plantarum and exopolysaccharide, which is applied in the field of biotechnology and food fermentation, and can solve the problems of poor adsorption effect and long adsorption time

Active Publication Date: 2017-08-18
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a kind of exopolysaccharide-producing Lactobacillus plantarum to solve the problems of poor adsorption effect and long adsorption time in the prior art.

Method used

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  • Lactobacillus plantarum strain capable of producing extracellular polysaccharide
  • Lactobacillus plantarum strain capable of producing extracellular polysaccharide
  • Lactobacillus plantarum strain capable of producing extracellular polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The isolation and screening of Lactobacillus plantarum LCC-605 comprises the following steps:

[0048] (1) Fermentation sample The liquid sample was taken from the homemade sauerkraut fermentation liquid in Qujing local family.

[0049] (2) Dilution of sample solution: Use a sterile 1mL pipette to draw 1mL sample solution into a sterile small test tube and dilute it with sterile normal saline to a dilution of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 、10 -9 、10 -10 、10 -11 、10 -12 . Choose a dilution of 10 -4 to 10 -12 spare.

[0050] (3) Separation of the plate: first, under the condition of aseptic operation on the ultra-clean bench, pour the sterilized MRS solid medium containing calcium carbonate into the plate, and draw the above dilution to 10 after the temperature is room temperature. -4 to 10 -12 0.1mL of fermentation broth. Pouring and coating onto the culture dish of the MRS solid medium containing calcium carbonate by pouring met...

Embodiment 2

[0052] Identification of Example 2 Bacterial Strain and Preparation of Exopolysaccharide

[0053] (1) Bacterial morphology observation and Gram staining

[0054] Use an inoculation loop to pick up a small amount of the matched bacterial sample prepared in Example 1 in the ultra-clean bench and spread it evenly on the glass slide, then heat it with an alcohol lamp to kill the bacteria, add ammonium oxalate crystal violet dropwise, and stain for 1 min. Rinse off excess staining solution on the washed slides with water, and blot dry with absorbent paper. Add a small amount of iodine-potassium iodide solution dropwise, let stand for 1min, then wash with water, blot dry with absorbent paper, add ethanol continuously dropwise for decolorization, until the outflowing liquid is colorless, then wash with water. Finally, counterstain with saffron stain for 30 s. After staining, observe with a microscope, Gram-positive bacteria are purple, and Gram-negative bacteria are red. The ident...

Embodiment 3

[0063] The detection of embodiment 3 heavy metal adsorption capacity:

[0064] (1) Effect of different carbon sources on biosorption effect

[0065] In order to explore the carbon source that makes the exopolysaccharide of lactic acid bacteria produce the best biosorption effect, MRS medium containing different carbon sources was used for fermentation. Lactic acid bacteria were cultured with MRS medium containing no carbon source and glucose, mannose, sucrose, and lactose respectively. After fermentation and extraction of exopolysaccharides, these six polysaccharides were prepared into 0.8mg / mL solutions and divided into 18 parts. Put it in a dialysis bag. Prepare 6 parts each of 10mg / L lead nitrate, cadmium nitrate and copper nitrate solutions, put the exopolysaccharide dialysis bags cultivated by 6 different carbon sources into the 3 kinds of ionic solutions respectively, and take samples of the ionic solutions after standing for 24 hours. Ion concentration detection. See...

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Abstract

The invention discloses a Lactobacillus plantarum strain LCC-605 capable of producing extracellular polysaccharide, which is stored in China Center for Type Culture Collection (No. CCTCC-M-2016491) on September 18, 2016. Compared with the strain in the prior art, the extracellular polysaccharide produced by Lactobacillus plantarum strain LCC-605 is found to be able to self-assemble to form nanoparticles, and has the highest capacity to adsorb heavy metal and methylene blue. The adsorptive capacities to methylene blue, Pb<2+>, Cd<2+> and Cu<2+> are 3,029 mg / g, 1,513 mg / g, 2,097 mg / g and 2,987 mg / g, respectively. The extracellular polysaccharide produced by Lactobacillus plantarum strain LCC-605 can be used for bioremediation to control the heavy metal and dye pollution in the environment, and has environmental friendliness and sustainable developability.

Description

technical field [0001] The invention belongs to the fields of biotechnology and food fermentation, and in particular relates to a plant lactobacillus producing extracellular polysaccharides. Background technique [0002] Lactic acid bacteria exopolysaccharide is a high-molecular polysaccharide polymer produced by lactic acid bacteria, which can be divided into capsular polysaccharide and exopolysaccharide. Capsular polysaccharides are generally distributed on the cell wall of bacteria, while exopolysaccharides are usually released into the culture medium. The exopolysaccharides of lactic acid bacteria have excellent applications in improving the rheological properties, texture and palatability of fermented dairy products (FEMSmicrobiology reviews, 1999, 23(2):153-177). The exopolysaccharides of lactic acid bacteria can exist in the intestine for a long time and thus increase the colonization of probiotics in the intestine (International dairy journal, 2002, 12(2): 163-171)....

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C02F1/28C12R1/25C02F101/22C02F101/20C02F101/30C02F101/36C02F101/38
CPCC02F1/285C02F2101/20C02F2101/22C02F2101/308C02F2101/36C02F2101/38C02F2101/40C12P19/04C12N1/205C12R2001/25
Inventor 林凤鸣李程程吕柔靖杨航田培龙
Owner SOUTHEAST UNIV
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