Tissue-culturing seedling growing method of sunflowers

Abstract

The invention belongs to the field of plant biotechnology and cultivation technology, and specifically relates to a method for cultivating seedlings of sunflower groups. Multi-branch varieties are selected as tissue culture materials, and axillary buds of plants whose top flowers are about to open or have opened are selected as explants. Body culture, induction and differentiation, strong seedling culture, rooting culture, tissue culture seedling domestication, transplanting to obtain high-purity sunflower tissue culture seedlings, overcame the difficulties in seed propagation of double-petal sunflower, low seed setting rate, difficult to stabilize offspring traits, separation Serious problem, the purity of tissue culture seedlings is high, the frequency of its induced budding is high, and the propagation speed is fast; Since sunflower is a cross-pollinated plant, it is easy to produce various sunflower mutants with high ornamental value in the offspring. The preservation of quality resources is of great significance.

Description

technical field [0001] The invention belongs to the field of plant biotechnology and cultivation technology, and in particular relates to a method for cultivating sunflower seedlings. Background technique [0002] Sunflower (Helianthus anuus L.) is a cultivar of the genus Helianthus of the family Compositae, which originated in the southwestern part of North America. Sunflower was introduced into my country at the end of the 16th century or the beginning of the 17th century. As a dried fruit, it has gradually become one of the main sources of edible oil in the diet structure of our people since the 1990s. In the early 1880s, sunflower began to be used as an ornamental plant in Europe. It has a cultivation history of more than 100 years. From the early 1980s, people began to study sunflower tissue culture. Until now, it is still very difficult to regenerate sunflower plants in vitro. Even if it can regenerate, the repeatability is very poor. The regeneration pathway of sunflowe...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problems of multi-petal multi-head sunflower varieties (yellow double-petal multi-head sunflower, purple double-petal multi-head sunflower, white double-petal multi-head sunflower) seed propagation difficulties, low seed setting rate, difficult to stabilize offspring traits, and serious separation problems, the present invention proposes a A method for cultivating sunflower tissue culture seedlings with axillary buds as explants, and established a sunflower regeneration system in vitro

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