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Medicated leaven fermentation process on-line quality detection system

A detection system and fermentation process technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of lagging control technology, lack of identification and content of active ingredients, poor quality stability of Liushenqu fermentation products, etc., to improve the quality The effect of pass rate

Active Publication Date: 2017-08-11
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the control of the traditional fermentation process of Liushenqu mainly relies on subjective experience, the control technology lags behind, and there is a lack of objective indicators and effective methods. The quality stability of Liushenqu fermentation products is not good, and the quality difference between different batches of products is large. Big
The Liushenqu produced by modern fermentation technology only controls the process parameters such as temperature and humidity in the fermentation process. The ministerial standards and local standards only have a simple description of prescription, preparation method, properties, usage and dosage, etc. The main body - the fermented material in dynamic changes does not carry out the detection of relevant quality indicators, lacks the identification of active ingredients and routine inspection items such as content, moisture, ash, and it is difficult to control the quality of medicinal materials

Method used

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  • Medicated leaven fermentation process on-line quality detection system
  • Medicated leaven fermentation process on-line quality detection system
  • Medicated leaven fermentation process on-line quality detection system

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1 The establishment method of high performance liquid chromatography online quality detection system of Liushenqu fermentation process of the present invention

[0032] 1 Materials and methods

[0033] 1.1 Preparation and sampling of Liushenqu samples

[0034] Put the Liushenqu raw materials into a constant temperature and humidity box for fermentation, keep the temperature at 30°C to 35°C and the humidity at 70% to 80%, and start timing. From the beginning of fermentation, sampling is carried out at regular intervals until the Liushenqu is fermented to the sample. The surface was covered with white mold for more than 130 hours, and the samples taken out were stored at -20°C for later use.

[0035] 1.2 HPLC data collection

[0036] 1.2.1 Preparation of the test solution

[0037] Weigh 5.00 g of the crushed sample into a conical flask, add 10 mL of methanol, weigh, extract by ultrasonic for 30 min, then make up for the lost methanol weight, filter the membr...

Embodiment 2

[0070] Example 2 Validation of the high-performance liquid chromatography on-line quality detection system in the fermentation process of Liushenqu according to the present invention

[0071] Put the Liushenqu raw materials into a constant temperature and humidity box for fermentation, keep the temperature at 30°C to 35°C and the humidity at 70% to 80%, and start timing. From the beginning of fermentation, sampling is carried out at regular intervals until the Liushenqu is fermented to the sample. The surface was covered with white mold for more than 130 hours, and the samples taken out were stored at -20°C for later use.

[0072] The conditions of high performance liquid chromatography are: chromatographic column: C18 (250mm×4.6mm, 5μm); mobile phase is acetonitrile (A) and water (B), linear gradient elution, elution program is 0~15min, 5%~ 10%(A); 15~50min, 10%~30%(A); 50~80min, 30%~60%(A); 80~100min, 60%~95%(A); 100~110min, 95 %(A); 110~120min, 95%~5%(A); 120~125min, 5%(A)...

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Abstract

The invention provides a medicated leaven fermentation process on-line quality detection system. A method comprises a step a of obtaining liquid chromatography data of each sample by adopting high performance liquid chromatography; respectively determining contents of artemisinin, quercetin, rutin and amygdalin in each sample; a step c of performing protease enzyme activity prediction: y=0.0021x1+3.1067x2-1.4643x3+0.0116x4+0.2095x5+27.6106; performing amylase enzyme activity prediction: y=0.3054x1+89.2468x2-16.0689x3-0.0748x4+0.2888x5+352.1262, wherein x1 is artemisinin, x2 is quercetin, x3 is rutin, x4 is amygdalin, x5 is fermentation time, and y is protease and / or amylase enzyme activity. The medicated leaven fermentation process on-line quality detection system is favorable for enabling users to learn about dynamic changes in fermentation process intermediate product quality in real time and favorable for optimizing intermediate product quality development direction through process parameter control, and thus the quality pass rate of a final product is improved.

Description

technical field [0001] The invention relates to a high-performance liquid chromatography on-line quality detection system in the fermentation process of Liushenqu. Background technique [0002] Liushenqu, also known as Liuqu and Divine Comedy, was first recorded in "Medicinal Nature" and is the most representative traditional Chinese medicine fermented processed product. , Bitter almond 100g, red bean 100g, wheat bran 5000g, flour 2500g, has the effect of strengthening the spleen and stomach, digestion and regulating. [0003] The application of fermentation technology in the processing of traditional Chinese medicine has a long history. It refers to the use of enzymes and microorganisms to decompose and catalyze the processed or purified medicinal materials or medicinal materials under certain humidity and temperature conditions. The concocting method of making medicine foam and undressing. [0004] At present, the control of the traditional fermentation process of Liushe...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张利杨程胡亮姜媛媛朱柏雨吴一超周永红王晓丽杨瑞武胡青青丁春邦苟丽琼佘启惠吴忠旺
Owner SICHUAN AGRI UNIV
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