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Fe3O4/octreotide modified nano-liposome and preparing method thereof

A nano-liposome and octreotide technology, which is applied in the field of medicine, can solve the problems of inability to realize targeted drug delivery and precise release, and achieve the effects of simple preparation process, improved therapeutic effect, and good therapeutic effect

Inactive Publication Date: 2017-08-11
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional liposome drug delivery system still has certain defects, such as the inability to achieve targeted drug delivery and precise release.

Method used

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  • Fe3O4/octreotide modified nano-liposome and preparing method thereof
  • Fe3O4/octreotide modified nano-liposome and preparing method thereof
  • Fe3O4/octreotide modified nano-liposome and preparing method thereof

Examples

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Effect test

Embodiment 1

[0034]Using the natural fat-soluble antineoplastic drug-oleanolic acid as a template drug, 1.0 mg oleanolic acid (purchased from Huakang Pharmaceutical Raw Material Factory, Shifang City, Sichuan Province), 40 mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd. ) and 4.0mg of cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) were dissolved in 2mL of absolute ethanol, and a lipid phase solution was formed by magnetic stirring; 1mg of PEG and 1mg of glutamic acid were dissolved in 8mL of phosphate buffer as water phase solution, adjust the pH of the aqueous phase solution to 6.0 with 1 mol / L NaOH solution, and add 8 μL Tween-80. Under magnetic stirring, slowly and uniformly drop 2 mL of lipid phase into 8 mL of water phase solution at 30°C at a speed of 80 rpm to obtain a liposome suspension, and finally continue rotary evaporation for 1.5 h to remove any impurities in the liposome suspension. water and ethanol to obtain ...

Embodiment 2

[0039] Taking natural fat-soluble antineoplastic drug-betulinic acid as a template drug, 1.5mg betulinic acid (purchased from Nanjing Zelang Pharmaceutical Technology Co., Ltd.), 45mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.) and 4.5 mg mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) was dissolved in 2 mL of absolute ethanol, and a lipid phase solution was formed by magnetic stirring. Dissolve 1.0 mg of PEG and 1.5 mg of glutamic acid in 9 mL of phosphate buffer as an aqueous phase solution, adjust the pH of the aqueous phase solution to 6.2 with a concentration of 1 mol / L NaOH solution, and add 11 μL of Tween-80. Under magnetic stirring, 2 mL of lipid phase was slowly and uniformly dropped into 9 mL of aqueous phase solution at 34° C. at a speed of 90 rpm to obtain a liposome suspension. Finally, the rotary evaporation was continued for 1.7 h to remove the absolute ethanol in the liposome suspension to obt...

Embodiment 3

[0044] Taking the natural fat-soluble antineoplastic drug-ursolic acid as a template drug, 3.0mg ursolic acid (purchased from Huakang Pharmaceutical Raw Material Factory, Shifang, Sichuan), 50mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.) and 5.0 mg of cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) was dissolved in 2 mL of absolute ethanol, and a lipid phase solution was formed by magnetic stirring. Dissolve 2.5mg of PEG and 4.5mg of glutamic acid in 10mL of phosphate buffer as the aqueous phase solution, adjust the pH of the aqueous phase solution to 7.0 with a concentration of 1mol / L NaOH solution, and add 12 μL of Tween-80. Under magnetic stirring, 2 mL of lipid phase was slowly and uniformly dropped into 10 mL of aqueous phase solution at 40° C. at a speed of 110 rpm to obtain a liposome suspension. Finally, the rotary evaporation was continued for 2 h to remove the absolute ethanol in the liposome suspensi...

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Abstract

A Fe3O4 / octreotide modified nano-liposome is an octreotide modified drug-loading nano-liposome with a particle size of 80-200 nm and with magnetic Fe3O4 nano particles on the surface. According to a preparing method of the Fe3O4 / octreotide modified nano-liposome, a drug-loading liposome is modified with L-glutamic acid, so that the surface of the drug-loading liposome is negatively charged, and the surface of the nano-liposome is then modified with octreotide through electrostatic interaction; and Fe3O4 nano particles solution is mixed with the nano-liposome modified with octreotide based on a certain volume, and the Fe3O4 / octreotide modified dual-target nano-liposome is prepared through interaction between PEG on the surface of Fe3O4 and the hydroxyl group on the surface of the lipidosome. The method is simple, operation is easy, the preparing condition is mild, the cost is low, adopted raw materials have no toxic or side effect on the human body, and the cancer treatment effect is improved by means of magnetic targeting, precise drug release and the specific binding capacity of octreotide to tumor cells.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to a drug-loaded liposome and a preparation method thereof. Background technique [0002] At present, the proportion of deaths caused by malignant tumors in the world is getting higher and higher, and the current treatment methods such as chemotherapy, radiotherapy, and surgical treatment have very large side effects on the body. Cancer treatments with toxic side effects remain a major problem in global cancer treatment. [0003] Studies have found that fat-soluble anti-tumor drugs, in addition to significant anti-tumor effects, also have a variety of biological effects, such as anti-inflammation, anti-oxidation, anti-virus, etc., with significant pharmaceutical value. However, its bioavailability in vivo is poor, and its blood half-life is short, which limits its application. Nanoliposome is a highly recognized new carrier system, which is composed of phospholipid bilayers and c...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K41/00A61K47/64A61K47/52A61K47/54A61P35/00A61K31/56
Inventor 高大威王倩倩朱瑞艳刘志伟刘艳平张旭武
Owner YANSHAN UNIV
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