Method for simultaneously measuring content of dextrose, chlorine and sodium in sodium chloride and dextrose injection through HPLC-CAD (High Performance Liquid Chromatography- Corona Chaged Aerosol Detection)

Abstract

The invention discloses a method for simultaneously measuring the content of dextrose, chlorine and sodium in sodium chloride and dextrose injection through HPLC-CAD (High Performance Liquid Chromatography- Corona Chaged Aerosol Detection) with advantages of measurement accuracy and simplicity in operation, belongs to the field of chemical detection and aims to provide a detection method which is accurate in measurement and simple in operation. The method sequentially comprises the following steps: 1) preparing a test solution, namely fixing the volume of 1ml of sodium chloride and dextrose injection to 1000ml with water, uniformly shaking, and filtering to obtain the solution; 2) preparing a reference solution, namely precisely weighing 12.5g of dextrose reference substance and 2.25g of sodium chloride reference substance, adding into a 250mL of measuring bottle, adding water for dissolving and diluting to scales, and uniformly shaking so as to obtain the reference solution; and 3) analyzing by using high performance liquid chromatography. The method can replace a traditional detection method for simultaneously measuring the content of dextrose, chlorine and sodium in the sodium chloride and dextrose injection.

Description

technical field [0001] The invention relates to the field of chemical detection, in particular to a method for simultaneously measuring the contents of glucose, chlorine and sodium in glucose sodium chloride injection by HPLC-CAD. Background technique [0002] The 2010 edition of the Chinese Pharmacopoeia records the method for the determination of glucose in glucose injection. Section 3.6.1 records that the content of glucose in the injection is determined by the optical polarimetry method. This method has high requirements for environmental temperature control and is less affected by environmental factors during determination Big, its section 3.6.2 records that the content of sodium chloride in the injection is determined by silver nitrate titration method. This method requires high professional skills of operators, and there are certain deviations in the test results of different testers. The existing national standard does not record the simultaneous measurement of gluco...

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Problems solved by technology

[0002] The 2010 edition of the Chinese Pharmacopoeia records the method for the determination of glucose in glucose injection. Section 3.6.1 records that the content of glucose in the injection is determined by the optical polarimetry method. This method has high requirements for environmental temperature control and is less affected by environmental factors during determination Large, its section 3.6.2 records the use of silver nitrate titration to determine the content of sodium chloride in the injection. This method requires high professional skills of the operators, and there are certain deviations in the test results of different testers.

The existing national standard does not record the simultaneous measurement of glucose, chloride, and sodium content in glucose sodium chloride injection. Even if the traditional HPLC measurement method is used, the linear rate does not obey a strict linear distribution in the entire measurement range. standard

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