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A strain of Acinetobacter baumannii and its application

A technology of Acinetobacter baumannii and microbial strains, applied in the direction of bacteria, biological water/sewage treatment, microorganisms, etc., can solve the limited selection of heterotrophic nitrification-aerobic denitrification strains, and the complicated process of biological removal of ammonia nitrogen and other problems, to achieve good ammonia nitrogen removal effect, reduce operation and maintenance costs, and simplify the treatment process

Inactive Publication Date: 2020-01-03
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies existing in the existing biological denitrification technology, the object of the present invention is to provide a strain of Acinetobacter baumannii ( Acinetobacter baumannii ) AL-6, which solves the problems that the current biological ammonia nitrogen removal process is complicated, and the selection of heterotrophic nitrification-aerobic denitrification bacterial strains is limited; Concentrated wastewater treatment, simultaneous and efficient nitrification and denitrification under aerobic conditions

Method used

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  • A strain of Acinetobacter baumannii and its application
  • A strain of Acinetobacter baumannii and its application
  • A strain of Acinetobacter baumannii and its application

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Embodiment 1

[0032] Embodiment 1: Isolation and identification of bacteria

[0033] 1. Isolation and purification of bacteria

[0034] The Acinetobacter baumannii AL-6 of the present invention is isolated from the activated sludge in the aeration tank of the Chongqing Jiguanshi Sewage Plant, and is obtained through repeated alternation of acclimatization and cultivation of liquid medium and scribing and screening of solid medium plate. The specific implementation steps are as follows:

[0035] 1) Pipette 1 mL of mud-water mixture from the sewage plant into 80 mL of ammonia-nitrogen liquid medium, and acclimatize at constant temperature for 72 h at 120 rpm and 30°C. Take 1 mL of the cultured bacterial liquid and transfer it to 80 mL of ammonia nitrogen liquid medium for the second acclimatization culture, which lasts for 48 h.

[0036] 2) Dilute the enriched and acclimatized bacterial solution, and the dilution factor is 10 -1 -10 -6 , spread the diluted bacterial solution on a plate co...

Embodiment 2

[0047] Implementation 2: Removal Ability of Acinetobacter baumannii AL-6 to Ammonia Nitrogen and Total Nitrogen in Wastewater with Different Ammonia Nitrogen Concentrations

[0048] Take NH 4 + -N is nitrogen source, CH 3 COONa is the carbon source, and NH in wastewater 4 + -N concentrations of 80 mg / L, 140 mg / L and 210 mg / L were used to investigate the growth of strain AL-6 and the removal ability of ammonia nitrogen and total nitrogen. Wastewater initial NH 4 + The -N concentration is 80 mg / L, the C / N is 10, the pH is controlled at 7~8, and cultured in a shaker at 30°C and 120 rpm. At 30 h, strain OD 600 increased to 1.2, at this time the TN removal rate reached 90.8%, and the NH 4 + The -N removal rate reached 99.9%, the intermediate product nitrate accumulation was 9.9 mg / L, and the nitrite accumulation was 0.1 mg / L. Wastewater initial NH 4 + -N concentration is 140 mg / L, C / N is 10, at 30 ℃, 120rpm, cultured for 53 hours, OD 600 rose to 1.6, TN removal rate re...

Embodiment 3

[0049] Embodiment 3: the removal ability of Acinetobacter baumannii AL-6 to nitrate nitrogen in wastewater

[0050] Acinetobacter baumannii AL-6 used nitrate as nitrogen source, and the aerobic denitrification ability of the strain was investigated by detecting the degradation of nitrogen source in wastewater. The initial TN concentration of nitrate wastewater was 80.8 mg / L, and the initial NO 3 - The -N concentration was 79.2 mg / L, the initial C / N ratio was 10, the pH was controlled at 7-8, and the culture was carried out at 30 °C and 120 rpm. After 48 h, TN removed 48.2%, NO 3 - -N removed 50.2%. The experimental results indicated that the strain AL-6 could carry out aerobic denitrification with nitrate nitrogen as the substrate under aerobic conditions. see attached Figure 4 .

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Abstract

The invention discloses a strain of Acinetobacter baumannii ( Acinetobacter baumannii )AL‑6 and its applications. The strain was deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms on November 8, 2016, and the preservation number is CGMCC No.13248. The inventive strain is capable of denitrification through heterotrophic nitrification-aerobic denitrification. Under aerobic conditions, with CH 3 COONa is the carbon source, NH 4 + ‑N is the nitrogen source, which can achieve 81.9~99.9% NH 4 + ‑N removal, 59.7~90.8% of TN removal; NO 3 ‑ ‑N is nitrogen source, NO 3 ‑ ‑N removal rate is 50.2%, TN removal rate is 48.2%; 2 ‑ ‑N is nitrogen source, NO 2 ‑ The ‑N removal rate was 50.3%, and the TN removal rate was 48.8%. Strain AL‑6 has a good application prospect in the field of wastewater treatment with high concentration of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a strain of Acinetobacter baumannii ( Acinetobacter baumannii ) AL-6, and its application in the treatment of high-concentration ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen wastewater. Background technique [0002] With the rapid advancement of urbanization, the problems of water resource shortage and pollution have become increasingly acute, among which the problem of ammonia nitrogen pollution is particularly prominent. According to the Bulletin on the State of the Environment in China, the total discharge of ammonia nitrogen in my country reached 2.385 million tons in 2014. The direct discharge of ammonia nitrogen into the water body will not only cause eutrophication and black odor of the water body; it will also increase the difficulty and cost of water treatment, affect domestic water and industrial water supply, and cause direct economi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/20C12N1/205C12R2001/01
Inventor 赵彬安强程丹阳丁雪松黄晓龙
Owner CHONGQING UNIV
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