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Bacterial strain with heterotrophic nitrification and aerobic denitrification function and application thereof

An aerobic denitrification and heterotrophic nitrification technology, which is applied in the field of environmental microorganisms, can solve the problems of water quality deterioration of water ecological balance, aquatic organisms poisoning, etc., and achieves obvious aerobic denitrification ability and good ammonia nitrogen removal effect.

Inactive Publication Date: 2021-08-10
FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the nitrogen content in the water body exceeds the environmental capacity of the receiving water body, the ecological balance of the water will be broken, which will cause the deterioration of the water quality and have a toxic effect on aquatic organisms.

Method used

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  • Bacterial strain with heterotrophic nitrification and aerobic denitrification function and application thereof
  • Bacterial strain with heterotrophic nitrification and aerobic denitrification function and application thereof
  • Bacterial strain with heterotrophic nitrification and aerobic denitrification function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A strain with heterotrophic nitrification and aerobic denitrification function, said strain is Achromobacter xylosoxidans pf1, collected in Anhui Lantian Special Turtle Co., Ltd., Dougou Town, Wuhu City, Anhui Province, which was collected in 2021 It was deposited in the China Center for Type Culture Collection on March 25, with the preservation number CCTCC NO: M2021273; the preservation address is Wuhan University Campus, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

[0034] 1. Isolation and purification of bacterial strains

[0035] The water sample collected from the turtle breeding pond was inserted into the heterotrophic nitrification enrichment medium at a volume ratio of 10% for enrichment and domestication, and cultured on a shaker at 30°C and 150r / min; every 48h according to 10% The volume ratio was replaced with fresh heterotrophic nitrification medium, and the culture continued for 20 days.

[0036] Wherein, the formula of heterotrophic...

Embodiment 2

[0046] Embodiment 2, the cultivation of Achromobacter pf1 and the performance analysis of denitrification

[0047] Pick the colonies of the re-screened bacteria isolated in Example 1 to the R2A liquid medium, culture them on an air-bath shaker at 200 rpm at 30°C for 18 hours, centrifuge them at 4000r / min for 2 minutes, collect the bacteria, and resuspend them with an equal amount of sterile water Bacteria were respectively inoculated into NM (heterotrophic nitrification medium), DMA (using nitrate as nitrogen source) and DMB (using nitrite as nitrogen source) liquid medium according to the inoculation amount of 1% (volume ratio). Cultivate in shake flasks at 30°C and 200rpm, take samples regularly to measure the optical density (OD600), total nitrogen (TN), ammonia nitrogen (NH 4 + -N), nitrate (NO 3 - -N) and nitrite (NO 2 - -N) concentration, draw the growth curve of the bacteria according to the OD600 value, and judge the denitrification performance of the strain by an...

Embodiment 3

[0054] Example 3: Practical denitrification application of Achromobacter pf1

[0055] Take 1000ml of turtle and turtle industrial culture wastewater with an initial COD of 760mg / L and add it to a 3L beaker, add 15g of sodium citrate, after adding carbon source, the COD of turtle and turtle industrial culture wastewater increases to 8160mg / L; The R2A medium in Example 2 was expanded to the logarithmic phase of pf1 wet bacteria, placed at room temperature (18-25° C.), and the dissolved oxygen was maintained above 2 ppm by an aeration device. At the same time, the turtle breeding wastewater not inoculated with pf1 was treated in the same way as the control group. After 24 hours, samples were taken, centrifuged to remove the precipitate, and the contents of total nitrogen, total phosphorus and COD in the supernatant were detected. The details are shown in the following table:

[0056] Table 1 Effect of Achromobacter pf1 on removing nitrogen and phosphorus in wastewater

[0057]...

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Abstract

The invention provides a bacterial strain with heterotrophic nitrification and aerobic denitrification functions and application. The bacterial strain is (Achromobacter xylosoxidans) pf1, the bacterial strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2021273. The bacterial strain can synchronously perform nitrification and denitrification for nitrogen removal by taking organic carbon as a carbon source under an aerobic condition, and is suitable for removal of organic wastewater containing ammonia nitrogen, nitrate and nitrite. In the ammonia nitrogen wastewater with the initial concentration of 175 mg / L, the total nitrogen removal rate can reach 100%, the accumulation of intermediate products is less, and secondary pollution is avoided; in nitrate or nitrite wastewater with the initial concentration of 100 mg / L, the total nitrogen removal rate can reach 87.1% or 65.1%. After the industrial turtle breeding wastewater is treated, nitrogen and phosphorus in the industrial turtle breeding wastewater can be remarkably removed.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to an Achromobacter strain with heterotrophic nitrification-aerobic denitrification functions and its application in the treatment of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen wastewater. Background technique [0002] At present, a major problem facing my country's water environment protection is that the concentration of ammonia nitrogen in the water far exceeds the self-purification capacity of the sewage. The national surface water environmental quality in the first half of 2016 shows that 11.3% of my country’s top ten river basins are inferior to Category V, of which COD, ammonia nitrogen and total phosphorus are the main pollution indicators, and the oxygen-consuming pollutant ammonia nitrogen has become a major factor affecting the environmental quality of my country’s surface water bodies. primary indicator. The severe situation of ammonia...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/30C02F101/16C02F101/38C02F101/30C12R1/025
CPCC02F3/34C02F3/302C02F3/308C02F2101/16C02F2101/163C02F2101/166C02F2101/38C02F2101/30
Inventor 高娜梁阳阳杨坤李静方婷赵秀侠卢文轩李书杨
Owner FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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