Bletilla striata self-assembled nanoparticles and preparation method and application thereof
A technology of nano-particles and bletilla striata glue, applied in the field of medicine, can solve problems such as difficult absorption, inability to rule out virus infection, poor environmental stability, etc., achieve good tissue adhesion, improve hemostatic effect, and good hemostatic performance
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Embodiment 1
[0035] Extraction and separation of bletilla striata gum:
[0036] (1) Take 300g of dried Bletilla striata tubers, crush them into small pieces of about 8 mesh, add 9000ml of purified water to soak at room temperature for 2 hours, heat and reflux for extraction for 2 hours, filter through a 40-mesh filter, and collect the filtrate; add 9000ml of purified water to the residue and soak at room temperature for 1 hour, Heat and reflux for extraction for 2 hours, filter through a 40-mesh filter, collect the filtrate, and combine the two extraction filtrates to obtain about 17200 ml of the first filtrate.
[0037] (2) Place the first filtrate above in a vacuum concentrator, and concentrate under reduced pressure at 70°C until it becomes thick, so that the content of the active ingredient glucomannan is about 30%, and about 400ml of the concentrated solution is obtained.
[0038] (3) Slowly add 143ml of 95% ethanol solution to the above concentrate, stir until the ethanol concentrati...
Embodiment 2
[0046] Extraction and separation of bletilla striata gum:
[0047] (1) Take 600g of dried Bletilla striata tubers, crush them into small pieces of about 6 mesh, add 12,000ml of purified water to soak at room temperature for 2 hours, heat and reflux for extraction for 2 hours, filter through a 40-mesh filter, and collect the filtrate; continue to add 10,000ml of purified water to soak at room temperature for 1 hour. Heat and reflux for extraction for 1.5 hours, filter through a 40-mesh filter, collect the filtrate, add 10,000 ml of purified water to the residue and soak at room temperature for 1 hour, heat and reflux for 1 hour, filter through a 40-mesh filter, collect the filtrate, and combine the extracted filtrates for 3 times to obtain the first filtrate of about 31200ml.
[0048] (2) Place the first filtrate above in a vacuum concentrator, and concentrate under reduced pressure at 65°C until it becomes thick, so that the content of the active ingredient glucomannan is abou...
Embodiment 3
[0057] Extraction and separation of bletilla striata gum:
[0058] (1) Take 300g of dried Bletilla striata tubers, crush them into small pieces of about 5 mesh, add 6000ml of purified water to soak at room temperature for 2 hours, heat and reflux to extract for 2 hours, filter through a 40 mesh filter, and collect the filtrate; continue to add 4500ml of purified water to soak at room temperature for 1 hour. Heated and refluxed for 1.5h, filtered through a 40-mesh filter, collected the filtrate, continued to add 4500ml of purified water to soak at room temperature for 0.5h, heated and refluxed for 1.0h, filtered through a 40-mesh filter, collected the filtrate, and combined the two extraction filtrates to obtain the first The filtrate is about 14100ml.
[0059] (2) Place the first filtrate above in a vacuum concentrator, and concentrate under reduced pressure at 75°C until it becomes thick, so that the content of the active ingredient glucomannan is about 28%, and about 430ml o...
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