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Detection device and detection method of antibiotics based on micro-fluidic chip

A technology of microfluidic chips and detection devices, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., can solve problems such as no substantive breakthroughs, and achieve the effect of overcoming the complexity of measuring instruments

Inactive Publication Date: 2017-07-11
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the rapid and on-line detection system for antibiotic residues based on microfluidic chips, which obtains the type and concentration information of the antibiotics to be tested through changes in optical signals, has not yet had a substantial breakthrough in the application field.

Method used

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  • Detection device and detection method of antibiotics based on micro-fluidic chip
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  • Detection device and detection method of antibiotics based on micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] In the microchannel of the PMMA chip, wash the microchannel of the microfluidic chip with deionized water, low-concentration sulfuric acid aqueous solution, low-concentration sodium hydroxide aqueous solution, and borate buffer solution with a pH of 10 for 5 minutes each, so as to contain 300 μg / L ampicillin Xilin was the analyte, borate buffer with pH 10 was used as electrophoresis running buffer, the concentration was 1×10 -5 mol / L 2,7-dibromohymercuryl fluorescent yellow sodium salt is used as a fluorescent dye, and an electric field of 150V / cm is applied at both ends of the sample cell, and the sample is loaded in 30s, and the voltage at both ends of the sample cell is adjusted to 100V / cm , and applied an electric field of 300V / cm at both ends of the buffer pool, and then measured the change of the fluorescence intensity of the detection pool with time, and found that the fluorescence intensity of the detection pool was significantly weakened at 123s. figure 2 show...

Embodiment 2

[0044] Repeat example 1, using the mixed solution of penicillin G and ampicillin containing 50 μg / L as the test object, the obtained detection cell fluorescence intensity varies with time, and it is found that the fluorescence intensity at the detection cell is significantly higher at 104s and 119s. Weakening, corresponding to the weakening of the fluorescence intensity due to the interaction of penicillin G, ampicillin and 2,7-dibromomercuryl fluorescent yellow sodium salt, see image 3 .

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Abstract

The invention discloses a detection device and a detection method of antibiotics based on a micro-fluidic chip. The device comprises a substrate, a separation channel, a buffer solution hole, two sample storage holes and a detection hole are formed on the substrate, one end of the separation channel respectively communicates with the buffer solution hole and the two sample storage holes through micro-channels, the other end of the separation channel communicates with the detection hole, the detection hole communicates with a waste liquid hole through a micro-channel, the position, close to the detection hole, of the substrate is provided with a micro-groove, an excitation light source and a fluorescence detector are arranged in the micro-groove, a sample injection electric field is formed between the two sample storage holes, a separation electric field is formed between the buffer solution hole and the detection hole, one end of the buffer solution hole is a high voltage end, and one end of the detection hole is a low voltage end. The detection device overcomes the shortcomings of large sample demand, narrow application field and high test cost of the traditional antibiotic detection devices, and realizes online qualitative and quantitative detection of the antibiotics.

Description

technical field [0001] The invention relates to a microfluidic chip separation detection technology, in particular to an antibiotic detection device and detection method based on a microfluidic chip. Background technique [0002] Since the first use of penicillin in 1941, thousands of antibiotics have been developed. China is a big producer and user of antibiotics, but for a long time the utilization rate of antibiotics in hospitals across the country is much higher than that of developed countries in Europe and America. Antibiotics have the characteristics of strong bactericidal activity and low toxicity and side effects in the process of animal feeding, and are widely used in the feeding process. Since the residue of antibiotics will cause serious safety problems such as drug resistance, allergies, and imbalance of human colony, international organizations and food safety management departments of various countries have formulated the maximum limit indicators of antibioti...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N35/00
CPCG01N21/6402G01N35/00584
Inventor 顾志鹏王腾飞陈红梅刘静静聂富强
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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