Quinolone antibiotic degrading bacterium and application thereof
A technology of quinolones and antibiotics, which is applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of quinolone antibiotics pollution, etc., and achieve the effects of strong adaptability to the environment, prolonging the life of bacteria, and good effects
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Embodiment 1
[0045] Embodiment 1: Isolation and identification of quinolone antibiotic degrading bacteria:
[0046] Inorganic salt medium (g L -1 ): K 2 HPO 4 5.8, KH 2 PO 4 4.5, (NH4) 2 SO 4 2.0, MgCl 2 0.16, CaCl 2 is 0.02, Na 2 MoO 4 2H 2 O is 0.0024, KNO 3 0.0012, FeCl 3 0.0018, MnCl 2 2H 2 O is 0.0015, pH 7.0.
[0047] Basal medium (g L -1 ): tryptone 10.0, yeast extract 5.0, NaCl 10.0, pH 7.5; the medium was sterilized at 121°C for 20 minutes before use.
[0048] Domestication of quinolone antibiotic-degrading flora:
[0049] Domestication is divided into three stages (such as figure 1 ), first put 100mL of inorganic salt culture medium in a 250mL Erlenmeyer flask, sterilize at 121°C for 20min, after cooling, add quinolone antibiotics ciprofloxacin stock solution and yeast extract under aseptic conditions to make The final concentrations were 5mg / L and 1.0g / L respectively. At the same time, 5g of collected manure samples (collected in a large livestock and poultry ...
Embodiment 2
[0064] Example 2: Preliminary research on the degradability of quinolone antibiotics ciprofloxacin (CIP) by Ochrobactrum sp.JOB
[0065] Preparation of seed suspension:
[0066] The bacteria were inoculated in the basal medium, cultured in a constant temperature shaker at 30°C and 120r / min for 24 hours, and then inoculated from the basal medium to expand the culture. Take the bacterial liquid after expansion and cultivation for a certain period of time, centrifuge at 6000r / min for 5min, discard the supernatant, wash the bacterial cells with phosphate buffer for 3 times, and prepare OD 600nm For the bacterial suspension of 1.0, follow-up experiments were carried out. Colony forming units (CFU / mL) of the bacterial suspension were quantified for the dilution plate count method.
[0067] Quantitative Analysis of Degradation Ability of Degrading Strains
[0068] The experimental medium is CIP inorganic salt medium, by adding CIP and external carbon source yeast extract to the in...
Embodiment 3
[0073] Prepare OD 600nm 0.8 to 1.0 of the bacterial suspension is added to the water body polluted by quinolone antibiotics, and the proportion of the bacterial suspension is 1% to 5% (v / v). Stir or ventilate continuously to accelerate the removal efficiency of quinolone antibiotics.
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