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Porcine pseudorabies virus aloe gel nano-emulsion adjuvant inactivated vaccine and preparation method thereof

A technology of porcine pseudorabies virus and aloe vera gel, which is applied in the direction of antiviral agents, biochemical equipment and methods, viruses, etc., can solve the problems of aseptic suppurative damage, stimulate Th2 type immune response, and low immune efficacy, so as to improve immunity Potency, Humoral Immunity Promotion, Cellular Immunity Promotion Effect

Active Publication Date: 2017-07-07
商丘美兰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the existing nanoemulsion adjuvants adopt vaccine adjuvants such as aluminum salts and oil emulsions, which have more side effects when used, such as causing severe vaccination reactions, forming aseptic suppurative damage, and producing externally visible edema, Problems such as swelling, induration, and necrosis, while oil-emulsion adjuvants and aluminum gel adjuvants can only stimulate Th2 type immune responses, and the immune efficacy is low

Method used

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  • Porcine pseudorabies virus aloe gel nano-emulsion adjuvant inactivated vaccine and preparation method thereof
  • Porcine pseudorabies virus aloe gel nano-emulsion adjuvant inactivated vaccine and preparation method thereof
  • Porcine pseudorabies virus aloe gel nano-emulsion adjuvant inactivated vaccine and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Screening of porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant inactivated vaccine prescription

[0035] (1) Screening of surfactants

[0036] Surfactants contain both hydrophilic groups and lipophilic groups, so most of them are amphiphilic molecules, and the hydrophilic and lipophilic properties of surfactants can be represented by the HLB value. The surfactants to be selected in the present invention include tween-20, tween-80, span-20, span-80, polyoxyethylene castor oil (EL-40), PEG-400, etc. Mixed with a certain amount of oil phase white oil, the constant temperature magnetic stirrer is constantly stirring, and at the same time, ultrapure water is slowly added and continuously stirred. At the beginning, the viscosity of the system is low. Since the water content is less than the oil content, water-in-oil (W / O) is formed. However, as the amount of water increases, the viscosity of the system suddenly increases, the surfactant forms a layere...

Embodiment 2

[0061] Example 2. Quality evaluation of porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant inactivated vaccine

[0062] (1) Determine the type of vaccine according to the diffusion speed of water-soluble dye methylene blue and oil-soluble dye Sudan red in nanoemulsion. Methylene blue is blue, and it is easy to diffuse in the water phase; Sudan red is red, and it is easy to diffuse in the oil phase; in the inactivated vaccine of porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant, if the blue dye diffuses faster than the red dye It is O / W type nanoemulsion inactivated vaccine, otherwise it is W / O type nanoemulsion inactivated vaccine.

[0063] (2) Viscosity measurement: Use a 1ml glass pipette (the inner diameter of the lower mouth is 1.2mm, and the inner diameter of the upper mouth is 2.7mm), and suck 1ml of the inactivated vaccine of porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant in the environment of 25 ℃, and make it. Vertical flow ou...

Embodiment 3

[0075] Embodiment 3, the safety detection of porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant inactivated vaccine

[0076] (1) Muscle irritation detection

[0077] The left and right quadriceps muscles of rabbits were aseptically injected with 1 mL of porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant inactivated vaccine and 1 mL of sodium chloride injection, and porcine pseudorabies white oil adjuvant inactivated vaccine was also set up. control group. 48 hours after injection of porcine pseudorabies virus inactivated vaccine with white oil adjuvant, the stimulation response of quadriceps femoris at the injection site was observed. The preparation method of porcine pseudorabies virus white oil adjuvant inactivated vaccine is as follows: after mixing 94% white oil and 6% Span 80, add 1% to 2% aluminum stearate, after sterilization, add sterile inactivated Porcine pseudorabies virus liquid and 4% Tween 80 were emulsified for 30min below 25°C to obtai...

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Abstract

The invention relates to a porcine pseudorabies virus aloe gel nano-emulsion adjuvant inactivated vaccine. The porcine pseudorabies virus aloe gel nano-emulsion adjuvant inactivated vaccine comprises the following raw materials in parts by weight: 10 to 20 parts of a surfactants, 10 to 20 parts of a cosurfactant, 10 to 20 parts of an oil phase, and 30 to 40 parts of an aqueous phase. According to the invention, distilled water commonly used in the aqueous phases is changed into aloe gel with multiple pharmacological activities and obvious immunomodulatory effects on the basis of the formula of a nano-emulsion adjuvant (comprising the surfactants, the cosurfactants, the oil phases and the aqueous phases), furthermore, CpG oligodeoxynucleotides which can induce a strong immune response and regulate two immune response types of Th1 and Th2 are added to not only overcome the defect that an oil emulsion adjuvant and an alhydrogel adjuvant can only stimulate a Th2 type immune response, and further improve the immune efficacy of the adjuvant.

Description

technical field [0001] The invention belongs to the technical field of veterinary vaccine adjuvants, in particular to an inactivated vaccine of porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant and a preparation method thereof. Background technique [0002] Nanoemulsion is a liquid-liquid dispersion system, which is a translucent or transparent emulsion composed of surfactant, co-surfactant, oil phase and water phase. system. Nanoemulsions can be divided into three types: type I water-in-oil nanoemulsions (W / O type), in which the inside of the nanoparticles is a water phase, which is wrapped by an outer film composed of external surfactants and the like, and dispersed in the oil phase; II Type Ⅲ oil-in-water nanoemulsion (O / W type), the inside of the nanoparticles is an oil phase, which is wrapped by an outer membrane composed of external surfactants, etc., and dispersed in the water phase; type III is a bicontinuous type nanoemulsion (W / W type). O / W or O / W / O ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/245A61K39/39A61K9/107A61P31/22A61P37/04
CPCA61K9/1075A61K39/12A61K39/39A61K47/44A61K2039/552A61K2039/55583A61K2039/57A61K2039/575C12N2710/16734
Inventor 姬星宇焦金英李国辉李自波
Owner 商丘美兰生物工程有限公司
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