Application of ganoderma extracts and ganoderma triterpenes monomers for treating autosomal dominant inheritance polycystic kidney diseases

A Ganoderma lucidum extract, dominant genetic technology, applied in the field of biomedicine

Active Publication Date: 2017-07-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no effective treatment for ADPKD, except for end-stage renal failure, which mainly relies on dialysis or kidney transplantation.

Method used

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  • Application of ganoderma extracts and ganoderma triterpenes monomers for treating autosomal dominant inheritance polycystic kidney diseases
  • Application of ganoderma extracts and ganoderma triterpenes monomers for treating autosomal dominant inheritance polycystic kidney diseases
  • Application of ganoderma extracts and ganoderma triterpenes monomers for treating autosomal dominant inheritance polycystic kidney diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Embodiment 1, preparation of Ganoderma lucidum total triterpenes (Ganoderma lucidum extract)

[0099] 1. Preparation of Ganoderma lucidum total triterpenes (Ganoderma lucidum extract) and Ganoderma lucidum triterpene monomer

[0100]1.1 Preparation of Ganoderma lucidum total triterpenes (GT, Ganoderma lucidum extract)

[0101] After pulverizing 20kg of red lucidum fruiting body, heat and reflux extraction with 95% (volume percentage concentration) ethanol aqueous solution for 3 times. To dryness, recover the solvent under reduced pressure to obtain 1Kg of ethanol extract (paste).

[0102] Suspend the ethanol extract in 5L of water to obtain an ethanol extract suspension; add ethyl acetate that is 1 times the volume of the ethanol extract suspension to the ethanol extract suspension for extraction, and collect the ethyl acetate phase; the remaining liquid Continue to extract with ethyl acetate that is 1 times the volume of the remaining liquid, and extract with ethyl a...

Embodiment 2

[0135] Embodiment 2, Ganoderma lucidum total triterpenoids to the inhibitory action of MDCK vesicle formation and growth

[0136] 1. The total triterpenes of Ganoderma lucidum can inhibit the formation of MDCK vesicles without affecting the total number of cell colonies

[0137] Vesicle formation inhibition assay:

[0138] Canine kidney cells (Madin-Darby canine kidney cells, MDCK) were cultured in three-dimensional Matrigel (Purecol Collagen, Inamed Biomaterials Fremont Company, Cat. No. 5409) in vitro. The culture medium 1 used was to add three-dimensional Matrigel, HEPES, penicillin and streptomycin obtained the three-dimensional matrigel concentration of 2.9mg / ml, HEPES concentration of 10mM, penicillin concentration of 100U / ml, streptomycin concentration of 100μg / ml culture solution, pH 7.4. Culture solution 2 is a culture solution with FBS concentration of 10% and forskolin concentration of 10 μM by adding FBS and forskolin (FSK, forskolin, Sigma company, product number...

Embodiment 3

[0144] Example 3. Determination of the vesicle inhibitory effect of Ganoderma lucidum total triterpenoids in the kidney through an in vitro embryonic kidney model

[0145] In the evening of the first day, C57BL / 6 mice over 6 weeks old (Experimental Animal Center, Peking University Health Science Center) were mated with males and females in the same cage at a ratio of 1:1. The plug indicates that the female mouse has been pregnant for half a day. The mice without the vaginal plug are divided into cages first, and then closed in the cage at night, and then observed on the second day; the pregnant female mice are fed alone for 13 days, and the transwell plate is used for the embryonic kidney on the 13th day (Corning Company, Cat. No. 3401) for cultivation.

[0146] Take the 13.5-day-old mouse embryonic kidney and place it in the upper chamber of the transwell, and add 8-Br-cAMP (Sigma Company, Cat. No. B-5386) with a final concentration of 100 μM and 10% fetal bovine Serum, L-gl...

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Abstract

The invention discloses application of ganoderma extracts and ganoderma triterpenes monomers for treating autosomal dominant inheritance polycystic kidney diseases. The ganoderma extracts are prepared through a preparation method of ganoderma extracts. The method comprises the steps that an ethanol water solution is used for backflow to extract ganoderma lucidum to obtain an ethanol extract; ethyl acetate is used for extracting the ethanol extract, an ethyl acetate phase is collected to obtain an ethyl acetate extract; a sodium bicarbonate water solution is used for extracting the ethyl acetate extract, and a water phase is collected to obtain a sodium bicarbonate extract; the ethyl acetate is used for extracting the sodium bicarbonate extract, and an ethyl acetate phase is collected to obtain the ganoderma extracts; the ganoderma triterpenes monomers are ganoderic acid C2 ethyl ester, lucidone D or / and ganoderic acid C2. An experiment proves that the ganoderma extracts and the ganoderma triterpenes monomers can restrain formation and growth of kidney vesicae, and can be used for treating the autosomal dominant inheritance polycystic kidney diseases.

Description

technical field [0001] The invention relates to the application of ganoderma lucidum extract and ganoderma triterpene monomer in the treatment of autosomal dominant polycystic kidney disease in the field of biomedicine. Background technique [0002] Ganoderma Triterpenes (GTs) are a class of triterpenoids found in Ganoderma lucidum. They are very important chemical and medicinal components in Ganoderma lucidum, and have significant physiological activities. As early as 1982, Kubota T et al. first isolated triterpenoids ganoderma acid A and ganoderma acid B from the fruiting bodies of Chizhi. So far, more than 300 kinds of triterpenoids have been isolated from the fruiting bodies, spores and hyphae of various Ganoderma lucidum, which belong to the secondary metabolites of Ganoderma lucidum, with a molecular weight range of 400-600kDa, most of which are isolated from red sesame. The main clinical medicines are red lucidum, pine and fir ganoderma and purple lucidum. The trit...

Claims

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Application Information

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IPC IPC(8): A61K36/074A61P13/12
CPCA61K36/074A61K2236/333A61K2236/55
Inventor 杨宝学苏丽敏周虹陈若芸康洁
Owner PEKING UNIV
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