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High efficiency and multi-stage supercritical CO2 extraction method for ganoderma lucidum spore oil

A technology of Ganoderma lucidum spore oil and Ganoderma lucidum spores, which is applied in the direction of oil/fat production and fat production, can solve problems such as pollution, hidden dangers of production safety, unsafety, etc., and achieves the improvement of wall breaking efficiency, wall breaking rate, and easy operation. Effect

Inactive Publication Date: 2017-06-20
GUANGZHOU KOHEALA BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the supercritical CO2 extraction technology needs higher pressure and longer extraction time to extract Ganoderma lucidum spore oil. Since the powder of Ganoderma lucidum spores is relatively fine, it is easy to cause spraying under high pressure conditions, which not only affects the extraction quality of spore oil, And there are also production safety hazards
In order to solve this problem, a granulation method is adopted in the prior art. For example, in the patent CN1239100C, the broken spore powder is used as a raw material, granulated and dried through a volume ratio of 5% to 95% ethanol aqueous solution. extremely unsafe
In the patent CN1094766C, spore powder is used as raw material, and water, gelatin, and starch are extruded at room temperature. This method adds more auxiliary materials, which not only affects the extraction efficiency of spore oil, but also may bring more pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] An efficient, multistage supercritical CO2 extraction method for preparing Ganoderma lucidum spore oil, the preparation method is as follows:

[0027] Step A, preferably Ganoderma lucidum spores, dried at 4°C for 5 hours;

[0028] Step B, putting the Ganoderma lucidum spores obtained in Step A into an ultrafine pulverizer for 40 minutes to obtain the newly broken Ganoderma lucidum spore powder;

[0029] Step C, enzymatically breaking the wall of the spore powder obtained in step B and a biological enzyme under suitable conditions, wherein the biological enzyme is any one of cellulase, pectinase, hemicellulase, lysozyme, protease, etc. or multiple compound enzymes, the amount of biological enzymes is 0.2%, the suitable conditions are temperature 45°C, pH value 6.5, and enzymolysis time 3 hours;

[0030] Step D, performing wet granulation on the spore powder in step C, drying at 35°C to obtain granules with a water content of less than 5%, wherein purified water is used ...

Embodiment 2

[0034] An efficient, multistage supercritical CO2 extraction method for preparing Ganoderma lucidum spore oil, the preparation method is as follows:

[0035] Step A, preferably Ganoderma lucidum spores, dried at 4°C for 5 hours;

[0036] Step B, putting the ganoderma lucidum spores obtained in step A into an ultrafine pulverizer for 30 minutes to obtain the initially broken ganoderma spore powder;

[0037] Step C, enzymatically breaking the wall of the spore powder obtained in step B and a biological enzyme under suitable conditions, wherein the biological enzyme is any one of cellulase, pectinase, hemicellulase, lysozyme, protease, etc. or multiple compound enzymes, the amount of biological enzymes is 0.1%, the suitable conditions are temperature 25°C, pH value 4.5, and enzymolysis time 1 hour;

[0038] Step D, performing wet granulation on the spore powder in step C, drying at 35°C to obtain granules with a water content of less than 5%, wherein purified water is used as a ...

Embodiment 3

[0042] An efficient, multistage supercritical CO2 extraction method for preparing Ganoderma lucidum spore oil, the preparation method is as follows:

[0043] Step A, preferably Ganoderma lucidum spores, dried at 40°C for 5 hours;

[0044] Step B, putting the Ganoderma lucidum spores obtained in Step A into an ultrafine pulverizer for 20 minutes to obtain the initially broken Ganoderma lucidum spore powder;

[0045]Step C, the spore powder obtained in step B and biological enzymes are enzymolyzed to break the wall under suitable conditions, wherein the biological enzymes are one of cellulase, pectinase, hemicellulase, lysozyme, protease, etc. or A variety of compound enzymes, the amount of biological enzymes is 0.3%, the suitable conditions are temperature 38°C, pH value 5.5, and enzymolysis time is 2 hours;

[0046] Step D, performing wet granulation on the spore powder in step C, drying at 35°C to obtain granules with a water content of less than 5%, wherein purified water i...

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Abstract

The invention relates to the technical field of Chinese herbal component extraction, in particular to a microwave enhanced supercritical CO2 extraction method for ganoderma lucidum spore oil, and the method adopts ganoderma lucidum spore as the raw material, and conducts superfine grinding and bio-enzyme method combined wall breaking. The superfine grinding and bio-enzyme method combined wall breaking technology adopted by the invention not only enhances the wall breaking rate, but also improves the wall breaking efficiency by 30%-50% than that of single wall breaking method, and the wall breaking rate reaches more than 99.7%. The adopted microwave enhanced supercritical CO2 extraction can complete extraction under a mild unvironment, the operation is simple and the cost is low. superfine grinding and bio-enzyme method combined wall breaking, wet granulation and microwave enhanced supercritical CO2 extraction are carried out on the ganoderma lucidum spore, the obtained ganoderma lucidum spore oil has an oil yield of 41.3%, which is 17.1% higher than that of supercritical CO2 extraction, and the content of ganoderma triterpene, ergosterol and other bioactive constituents can be increased by 10%-20%.

Description

technical field [0001] The invention relates to the technical field of extraction of traditional Chinese medicine components, in particular to a method for preparing ganoderma lucidum spore oil by using ganoderma lucidum spores as raw materials through ultrafine pulverization and bioenzyme combined wall breaking and microwave-enhanced supercritical CO2 extraction. Background technique [0002] Ganoderma lucidum has a very long history as a medicine. As early as the Eastern Han Dynasty, my country’s first medicinal book "Shen Nong’s Herbal Classic" recorded: Ganoderma lucidum has six kinds of purple, red, green, yellow, white, and black, and its nature and taste are sweet and flat. It is mainly used to treat "the knot in the chest, benefit the heart, nourish the middle, increase wisdom, not forget, eat for a long time, lighten the body and not grow old, prolong life and immortality", and list Ganoderma lucidum as the top grade. The annual edition of "Pharmacopoeia of the Peopl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04
Inventor 葛啸虎陈海佳王一飞杨占东
Owner GUANGZHOU KOHEALA BIOTECHNOLOGY CO LTD
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