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Culture medium for culturing antrodia camphorata by vessel culturing and preparation method thereof

A culture medium, Antrodia camphorata technology, applied in botanical equipment and methods, cultivation, plant cultivation and other directions, can solve problems such as being unsuitable for large-scale production, slow growth, easy to infect miscellaneous bacteria, etc., to avoid heavy metal pollution and simplify cultivation. procedures, the effect of reducing bacterial contamination

Active Publication Date: 2017-06-20
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dish culture uses the culture dish as the culture container, and the culture conditions are highly controllable. It can not only reduce the cross-contamination of bacteria between units, but also avoid heavy metal pollution, which helps to increase the output per unit of cultivation area, reduce production costs, and is easy to use. Quantitative production has been realized, but the Antrodia camphorata cultivated on the current culture medium of dish culture has defects such as slow growth rate, susceptibility to miscellaneous bacteria, low yield, and being unsuitable for large-scale production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0017] The preparation method of the malt extract is as follows: crush the malt, add 5 times the weight of deionized water, filter with a 0.5mm filter, stir and mix gently at room temperature for 2h, 6000g, 30min, remove the supernatant, and use hydrochloric acid Adjust the pH to 7.2, autoclave at 121°C for 15 minutes, and freeze-dry to powder.

[0018] The preparation method of the Cinnamomum plant mixture is as follows: the Cinnamomum plant is first crushed into powder, dried at 60°C, added with ultrapure water at 121°C for 20 minutes, filtered and then extracted once more. The plant of the genus Cinnamomum is one or more of camphor tree, cinnamon camphor, camphor, sassafras, camphor, camphor, and cinnamon.

[0019] The preparation method of the golden moss brittle leaf extract is as follows: crush the golden moss brittle leaf, add 5 times the weight of deionized water, filter with a 0.5mm filter, stir and mix gently at room temperature for 2h, 6000g, 30min , remove the sup...

Embodiment 1

[0022] A culture medium of Antrodia camphorata, made from the following raw materials, potato extract powder 250g, glucose 30g, soluble soybean powder 10g, malt extract 10g, magnesium sulfate 1g, camphor plant mixture 30g, potassium dihydrogen phosphate 1g, vitamin B10 .03g, chloramphenicol 0.05g, agar 20g, ultrapure water 1L.

[0023] Prepare the Cinnamomum plant mixture first, then dissolve and mix the components, adjust the pH to 5.0, and sterilize under high pressure at 121°C for 20 minutes. Pour the sterilized medium into a plate on an ultra-clean workbench with 25ml of medium per plate, and wait for the medium to After coagulation, inoculate Antrodia camphorata strains, and culture at 25°C for 140 days to harvest mycelia or fruiting bodies.

Embodiment 2

[0025] Antrodia camphorata dish culture medium is made from the following raw materials, potato extract powder 200g, glucose 20g, soluble soybean powder 2g, malt extract 3g, magnesium sulfate 0.5g, camphor plant mixture 10g, potassium dihydrogen phosphate 0.5g, Vitamin B10.01g, chloramphenicol 0.01g, agar 15g, ultrapure water 1L.

[0026] Prepare the Cinnamomum plant mixture first, then dissolve and mix the components, adjust the pH to 4.5, and sterilize under high pressure at 121°C for 20 minutes. Pour the sterilized medium into a plate on an ultra-clean workbench with 20ml of medium per plate, and wait for the medium to After coagulation, inoculate the strain of Antrodia camphorata, cultivate at 20°C for 180 days, and harvest mycelium or fruiting bodies.

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PUM

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Abstract

The invention discloses a culture medium for culturing antrodia camphorata by vessel culturing and a preparation method thereof. The culture medium is prepared from the following raw materials in parts by weight: 200 to 300 parts of potato leaching powder, 20 to 40 parts of glucose, 0 to 20 parts of soluble soybean meal, 1 to 20 parts of malt extractant, 0.5 to 1.5 parts of magnesium sulfate, 10 to 60 parts of a mixture of a cinnamomum plant, 0.5 to 1.5 parts of potassium dihydrogen phosphate, 0.01 to 0.05 part of vitamin B1, 0 to 0.1 part of chloramphenicol, 15 to 25 parts of agar and 1,000 parts of ultrapure water. According to the culture medium disclosed by the invention, a culturing vessel is used for culturing the antrodia camphorata; the culturing condition has strong controllability; the culturing procedure is simplified; the sundry bacteria contamination can be reduced; the heavy metal pollution is avoided too; the growth increment of antrodia camphorata mycelium or sporocarp is increased as well; and the production cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of fungal culture, and in particular relates to a dish culture medium of Antrodia camphorata and a preparation method thereof. Background technique [0002] Antrodia camphorata (Antrodia camphorata), also known as Antrodia camphorata, Cinnamomum camphorata, Camphor mushroom or Red camphor mushroom, belongs to Basidiomycetes, Polyporaceae, Polyporaceae, and Thinporus genus. It was discovered in Taiwan, China in the 1990s The edible (medicine) fungus has the reputation of "forest ruby". Antrodia Cinnamomea has a variety of physiologically active components, including polysaccharides, sterols, triterpenes, adenosines, unsaturated fatty acids, and immune proteins. Studies in recent years have confirmed that Antrodia camphorata has the functions of relieving alcohol and protecting the liver, eliminating tumors, tonifying the kidney and strengthening the heart, benefiting the stomach and intestines, strengthening...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04
CPCA01G18/00C05B7/00C05G3/00C05D5/00C05F11/00C05F11/10
Inventor 孟红岩郭莺陈菲林志楷刘黎卿洪翠云马冬菊林文珍
Owner SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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