Culture medium for culturing antrodia camphorata by vessel culturing and preparation method thereof
A culture medium, Antrodia camphorata technology, applied in botanical equipment and methods, cultivation, plant cultivation and other directions, can solve problems such as being unsuitable for large-scale production, slow growth, easy to infect miscellaneous bacteria, etc., to avoid heavy metal pollution and simplify cultivation. procedures, the effect of reducing bacterial contamination
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[0017] The preparation method of the malt extract is as follows: crush the malt, add 5 times the weight of deionized water, filter with a 0.5mm filter, stir and mix gently at room temperature for 2h, 6000g, 30min, remove the supernatant, and use hydrochloric acid Adjust the pH to 7.2, autoclave at 121°C for 15 minutes, and freeze-dry to powder.
[0018] The preparation method of the Cinnamomum plant mixture is as follows: the Cinnamomum plant is first crushed into powder, dried at 60°C, added with ultrapure water at 121°C for 20 minutes, filtered and then extracted once more. The plant of the genus Cinnamomum is one or more of camphor tree, cinnamon camphor, camphor, sassafras, camphor, camphor, and cinnamon.
[0019] The preparation method of the golden moss brittle leaf extract is as follows: crush the golden moss brittle leaf, add 5 times the weight of deionized water, filter with a 0.5mm filter, stir and mix gently at room temperature for 2h, 6000g, 30min , remove the sup...
Embodiment 1
[0022] A culture medium of Antrodia camphorata, made from the following raw materials, potato extract powder 250g, glucose 30g, soluble soybean powder 10g, malt extract 10g, magnesium sulfate 1g, camphor plant mixture 30g, potassium dihydrogen phosphate 1g, vitamin B10 .03g, chloramphenicol 0.05g, agar 20g, ultrapure water 1L.
[0023] Prepare the Cinnamomum plant mixture first, then dissolve and mix the components, adjust the pH to 5.0, and sterilize under high pressure at 121°C for 20 minutes. Pour the sterilized medium into a plate on an ultra-clean workbench with 25ml of medium per plate, and wait for the medium to After coagulation, inoculate Antrodia camphorata strains, and culture at 25°C for 140 days to harvest mycelia or fruiting bodies.
Embodiment 2
[0025] Antrodia camphorata dish culture medium is made from the following raw materials, potato extract powder 200g, glucose 20g, soluble soybean powder 2g, malt extract 3g, magnesium sulfate 0.5g, camphor plant mixture 10g, potassium dihydrogen phosphate 0.5g, Vitamin B10.01g, chloramphenicol 0.01g, agar 15g, ultrapure water 1L.
[0026] Prepare the Cinnamomum plant mixture first, then dissolve and mix the components, adjust the pH to 4.5, and sterilize under high pressure at 121°C for 20 minutes. Pour the sterilized medium into a plate on an ultra-clean workbench with 20ml of medium per plate, and wait for the medium to After coagulation, inoculate the strain of Antrodia camphorata, cultivate at 20°C for 180 days, and harvest mycelium or fruiting bodies.
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