Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SNP typing method and kit

A typing method and kit technology, applied in the field of DNA detection, can solve problems such as false signals, and achieve the effect of reducing false signals

Inactive Publication Date: 2017-06-13
GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a SNP typing method and kit with higher accuracy for the detection of SNP sites in CYP2C9, VKORC1 and CYP2C19 genes, aiming to solve the high proportion of errors in the sequencing results in the prior art Signal technical issues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP typing method and kit
  • SNP typing method and kit
  • SNP typing method and kit

Examples

Experimental program
Comparison scheme
Effect test

no. 3 example

[0083] Based on the third embodiment, the present invention also proposes a fourth embodiment, the ligation reaction in the step B1 is carried out in a cutting-ligation reaction system, and the cutting-ligation reaction system includes: ligase, cleavage agent, immobilized First linker and ligation buffer on microspheres;

[0084] The cleaving agent is used to specifically cut the cleavable site or excisable sequence in the nucleic acid fragment to form the first cohesive end;

[0085] The first linker is a nucleic acid molecule containing a second cohesive end that is completely complementary to the first cohesive end.

[0086] It should be noted that the cleavage-ligation reaction system is a reaction system in which the ligase and the cleavage agent are active at the same time.

[0087] Through the above-mentioned special design of PCR amplification primers and cutting-ligation reaction system, in the process of library construction, the cutting and ligation reactions after...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of DNA detection and provides an SNP typing method and kit. A to-be-tested SNP site comprises SNP sites in CYP2C9, VKORC1 and CYP2C19 genes. The SNP typing method comprises the following steps: replacing a fluorescently-labeled oligonucleotide probe with a non-fluorescently-labeled oligonucleotide probe, performing one-time connection sequencing reaction on to-be-sequenced library molecules and then obtaining sequence information of the to-be-tested SNP site through connection sequencing. The SNP typing kit comprises a CYP2C9 specific primer pair, a VKORC1 specific primer pair, a CYP2C19 specific primer pair and the non-fluorescently-labeled oligonucleotide probe. The SNP typing method and kit provided by the invention have the benefit that through sealing non-specific binding sites in the to-be-sequenced library molecules, a false signal in a result obtained by the connection sequencing is reduced, so that the accuracy of SNP site detection is improved.

Description

technical field [0001] The invention relates to the technical field of DNA detection, in particular to a SNP typing method and a kit. Background technique [0002] Single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP) refers to the existence of conversion, transversion, insertion, deletion and other changes at a specific nucleotide position in DNA in the genome. At present, common SNP typing detection technologies at home and abroad include the following, high resolution melting curve detection (high resolution melting, HRM), SNP typing method based on chip technology, allele-specific amplification method (Amplification Refractory Mutation System Real Time PCR, ARMS-RT PCR), Taqman fluorescent probe method, mass spectrometry, high performance liquid chromatography and gene sequencing, etc. [0003] Among them, the gene sequencing method also includes the first-generation sequencing technology based on Sanger technology, and the second-generation high-throughp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2563/149C12Q2525/191C12Q2565/518
Inventor 盛司潼邓波
Owner GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com