Method for extracting fucoidan through biological enzymolysis process

Abstract

The invention belongs to the technical field of fucoidan extraction and provides a method for extracting fucoidan through a biological enzymolysis process. The method comprises the following steps: grinding kelp into slurry; soaking; adding acidic cellulase and hemicellulase; adding alkaline pectinase; adding protease; adding sodium chloride and water and reacting, thereby acquiring sodium alginate jelly; adding modified attapulgite; centrifuging by using a centrifugal machine; and adding ethyl alcohol and dewatering, thereby acquiring solid-phase fucoidan. According to the method for extracting fucoidan provided by the invention, the biological enzymolysis process is adopted; on the basis of the structure and ingredients of kelp cytoderm, a defined amount of related enzyme is added in stages, so that the cytoderm is thoroughly split; the fucoidan therein is fully released, so that the extraction rate of the fucoidan is increased; the modified attapulgite is added for decolorizing and removing fishy smell in an extraction process, so that the appearance of the product is cleaner; and besides, the extraction steps are simplified, the use of the chemical raw materials is reduced and the purpose of environmental protection is achieved.

Description

technical field [0001] The invention belongs to the technical field of extracting seaweed polysaccharides, and in particular relates to a method for extracting fucoidan by a biological enzymatic hydrolysis method. Background technique [0002] Seaweed is algae that grows in the sea. Generally speaking, algae can be divided into four types: green algae, brown algae, red algae and blue algae. They are cryptogamous plants in the plant kingdom. Algae include several different types that produce energy through photosynthesis biology. Seaweed is rich in nutrients and functional ingredients. Alginate or seaweed polysaccharide extracted from seaweed has special emulsification, gelling properties, medicinal value, immunity enhancement and anticancer activity, so it is gradually used in industry and pharmaceutical industry middle. [0003] Seaweed polysaccharides have the effects of inhibiting viruses, lowering cholesterol, lowering blood sugar and blood lipids. Among them, fucoida...

AI Technical Summary

Problems solved by technology

[0009] (1) The above methods cannot completely destroy the cell wall structure, but fully release the fucoidan in the cell wall, and the extraction rate is low;

[0010] (2) The appearance of the product is light yellow, not beautiful enough, and there is still an algae smell, which affects the reuse of the product;

[0011] (3) Use a large amount of organic and inorganic solutions such as ethanol, acetone, chloroform and methanol for treatment, which is not environmentally friendly

At present, there are still relatively few methods for extracting fucoidan by enzymatic hydrolysis in China, but there are also defects of low content of effective substances.