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A kind of preparation method and application of mycorrhizal fungus soil for cultivating blueberries

A mycorrhizal fungus, blueberry technology, applied in the field of mycorrhizal fungal soil preparation, can solve the problems of blueberry mycorrhizal formation difficulties, inoculation impact, mycorrhizal fungal infection rate is less than 3%, to overcome the limitations of geographical distribution Sexuality, convenient material collection, and the effect of increasing the infection rate

Active Publication Date: 2019-12-03
DALIAN UNIV
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  • Description
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  • Application Information

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Problems solved by technology

[0007] Under natural conditions, wild blueberries are rich in mycorrhizal fungi, and their infection rate can reach 75%. However, it is difficult for blueberries to form mycorrhizae under artificial cultivation conditions, and their mycorrhizal fungal infection rate is less than 3%.
However, due to the geographical limitation of the distribution of wild blueberries, the use of wild blueberries to cultivate mycorrhizal fungi and the inoculation of cultivated blueberries are also affected to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Sampling of mycorrhizal fungi: Select a vigorous Rhododendron tree in Dahei Mountain, Dalian, and dig down at 20cm around the trunk to the main root system distribution (depending on the size of the tree, generally 30-40cm deep, the deeper the tree The deeper the main root system is distributed), observe whether there are mycorrhizae (most rhododendrons have heather mycorrhizae). Around the mycorrhizae, soil samples containing some fine roots below 2mm were taken.

[0029] 2. Prepare Martin's medium, the composition of Martin's medium is as follows: KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, peptone 5g, glucose 10g, agar 15g, water 1000ml, pH natural, 1% Bengal red aqueous solution 3.3ml. This medium is conducive to the growth of mycorrhizal fungi.

[0030] 3. Preparation of soil dilution: Use a 20-mesh sieve to filter the soil sample taken, weigh the filtered soil sample together with 10g of fine roots, put it into a triangular flask filled with 90ml sterile water, an...

Embodiment 2

[0037] The difference between this embodiment and embodiment 1 is that the concentration of streptomycin and the cooling temperature in step 4 are different.

[0038] Step 4 of this embodiment is: melt the Martin's medium, and when it is cooled to 60° C., add a streptomycin solution with a concentration of 12 mg / ml to the medium, so that each ml of medium contains 60 μg of streptomycin. Then, inject about 15ml of culture medium into the petri dish under aseptic operation, shake the petri dish gently after covering, so that the culture medium is evenly distributed, place it flat on the table, and form a flat plate after coagulation.

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Abstract

The invention belongs to the technical field of fruit culturing and particularly relates to a preparation method and application of blueberry culturing mycorrhiza fungi soil. According to the preparation method and application of the blueberry culturing mycorrhiza fungi soil, by collecting a soil sample of ericoid mycorrhiza fungi, performing separation and purification on the fungi and returning the fungi to the root systems of blueberry plants, the infection rate of mycorrhiza can reach up to 52%, while that of the mycorrhiza of normally-planted blueberry is less than 3%. The preparation method of the blueberry culturing mycorrhiza fungi soil can is significant in increasing the infection rate of the mycorrhiza of blueberry and meanwhile is convenient in material-obtain and capable of overcoming limitations led by geographical distribution of blueberry mycorrhiza, thereby giving full play to the benefits of the mycorrhiza.

Description

technical field [0001] The invention belongs to the technical field of fruit tree cultivation, and in particular relates to a preparation method and application of mycorrhizal fungus soil for cultivating blueberries. Background technique [0002] Mycorrhizal fungi play an important role in the nutrient cycle and material cycle of the ecosystem, and play a very important role in the nutrient acquisition and growth of plants. First of all, mycorrhizal fungi can promote the growth of plants and promote the absorption of nitrogen and phosphorus elements by plants. Secondly, mycorrhizal fungi can directly synthesize or induce plants to produce a variety of hormones during the growth of mycorrhizal fungi and their symbiosis with plants. gender plays an important role. [0003] Mycorrhizal fungi in soil form a mutualistic symbiosis with host roots called mycorrhizae. The research results confirm that VA mycorrhizae can increase the absorption of mineral nutrients, especially phos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/10A01G24/20A01G24/00A01G17/00
CPCA01G17/005A01G18/10A01G24/00
Inventor 李根柱张自川娄鑫王贺新
Owner DALIAN UNIV
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