Blood glucose reducing and islet protecting activity test method for horseradish tree leaf flavonoids extract and application thereof

A technology for testing the flavonoids and activity of the leaves, which is applied in the field of biomedicine to achieve the effects of reducing glucose tolerance, saving costs, and high extraction efficiency

Inactive Publication Date: 2017-05-31
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports on the hypoglycemic and islet-protective activity tests of Moringa leaf flavonoids extract and

Method used

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  • Blood glucose reducing and islet protecting activity test method for horseradish tree leaf flavonoids extract and application thereof
  • Blood glucose reducing and islet protecting activity test method for horseradish tree leaf flavonoids extract and application thereof
  • Blood glucose reducing and islet protecting activity test method for horseradish tree leaf flavonoids extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The hypoglycemic and islet protective activity test method of Moringa oleifera flavonoids extract comprises the following steps:

[0047] S1. Preparation of flavonoid extract: Weigh 100mg of flavonoid powder from Moringa oleifera leaves, dilute to 1mL, mix well, filter through a 0.22um filter, divide into 5 equal samples, and set aside;

[0048] S2. Glucose uptake test: Digest the HepG2 cell solution for the test with 0.25% trypsin solution, inoculate it on a culture plate, and place it together in CO 2 cultured in an incubator, wherein the cell concentration of the HepG2 cell solution was 1.0×10 5 cells / mL, after the cells adhered to the wall, the original culture solution was discarded, and the initial glucose concentration in the culture solution of each well was measured with a glucose kit, calculated as G 1 , change the serum-free culture medium, starve for 10 h, add 180 μL of DMEM high-glucose culture medium, and then add 30 μL of samples respectively, after incu...

Embodiment 2

[0057] The hypoglycemic and islet protective activity test method of Moringa oleifera flavonoids extract comprises the following steps:

[0058] S1. Preparation of flavonoids extract: Weigh 80 mg of flavonoids powder from Moringa oleifera leaves, dilute to 1 mL, mix well, filter through a 0.22um filter, divide into 5 equal samples, and set aside;

[0059] S2. Glucose uptake test: Digest the HepG2 cell solution for the test with 0.25% trypsin solution, inoculate it on a culture plate, and place it together in CO 2 cultured in an incubator, wherein the cell concentration of the HepG2 cell solution was 0.8×10 5 cells / mL, after the cells adhered to the wall, the original culture solution was discarded, and the initial glucose concentration in the culture solution of each well was measured with a glucose kit, calculated as G 1 , change the serum-free culture medium, starve for 12 hours, add 160 μL of DMEM high-glucose culture solution, and then add 20 μL of samples respectively, a...

Embodiment 3

[0067] The hypoglycemic and islet protective activity test method of Moringa oleifera flavonoids extract comprises the following steps:

[0068] S1. Preparation of flavonoids extract: Weigh 90 mg of flavonoids powder from Moringa oleifera leaves, dilute to 1 mL, mix well, filter through a 0.22um filter, divide into 5 equal samples, and set aside;

[0069] S2. Glucose uptake test: Digest the HepG2 cell solution for the test with 0.25% trypsin solution, inoculate it on a culture plate, and place it together in CO 2 cultured in an incubator, wherein the cell concentration of the HepG2 cell solution was 1.5×10 5 cells / mL, after the cells adhered to the wall, the original culture solution was discarded, and the initial glucose concentration in the culture solution of each well was measured with a glucose kit, calculated as G 1 , change the serum-free culture medium, starve for 12 hours, add 200 μL of DMEM high-glucose culture medium, and then add 50 μL of samples respectively, aft...

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Abstract

The invention discloses a blood glucose reducing and islet protecting activity test method for a horseradish tree leaf flavonoids extract and application thereof. The blood glucose reducing and islet protecting activity test method for the horseradish tree leaf flavonoids extract comprises the following steps: S1, preparing a flavonoids extract; S2, glucose consumption test: carrying out glucose consumption test according to a glucose consumption activity coefficient K; S3, glucolipid metabolism activity test: calculating a rat blood lipid index, measuring rat serum SOD and MDA contents and calculating an insulin index; and S4, islet protecting activity test: calculating islet cell quantity change rate M and islet beta cell growth rate N. By the method, the glucose consumption activity coefficient K is calculated, the blood glucose change rate of rats is measured, and the rat blood lipid index, the rat serum SOD and MDA contents, the insulin index, the islet cell quantity change rate M and the islet beta cell growth rate N are measured, the blood glucose reducing and islet protecting activity index of the horseradish tree leaf flavonoids extract is judged comprehensively, and therefore, a quantitative standard is provided for utilization and development of horseradish tree leaves.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a test method for hypoglycemic and islet-protective activity of flavonoids from leaves of Moringa oleifera and its application. Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is caused by defective insulin secretion or impaired biological action, or both. The long-term high blood sugar in diabetes leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. There is currently no cure for diabetes, but diabetes can be controlled through a variety of treatments. The research on natural plant hypoglycemic has become more and more popular. Studies have shown that Moringa leaf powder also has the effect of lowering blood sugar. Moringa (moringa oleifera) belongs to the Moringaceae family and the genus Moringa, which originated in the sub-Himalaya...

Claims

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Application Information

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IPC IPC(8): C12Q1/02A61K49/00A61K36/185A61P3/10
CPCG01N33/5038A61K36/185A61K49/0008G01N33/507
Inventor 汪开毓吉莉莉王乙力黄小丽贺扬
Owner SICHUAN AGRI UNIV
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