Maize MS8 gene mutant as well as molecular identification method and application thereof

Abstract

The invention provides a maize Ms8 gene mutant as well as a molecular identification method and application thereof, belonging to the technical field of gene engineering. A waxy corn variety namely Jingkenuo 2000 is subjected to cobalt60 radiation induced mutation to induce a basic group T in a sixth exon of a maize beta-1,3-galactosyl transferase (Ms8) gene to mutate into a basic group C, thereby resulting in that a tyrosine is transformed into a histidine. The Ms8 gene mutant is named as ms8-4505, a nucleotide sequence thereof is shown by SEQ ID No.1, the mutant is further proved to cause maize recessive male sterility, and the mutant can be used for preparing a maize selfing line and transgenic maize with recessive male sterility, and plays an important role in genetic improvement breeding of maize germplasm resources. The invention also provides a molecular marker identification method of the mutant and application of the mutant in breeding and seed production.

Description

technical field [0001] The invention belongs to the fields of plant molecular biology and genetic engineering, and in particular relates to a corn MS8 gene mutant ms8-4505 and its molecular identification method and application. Background technique [0002] Plant male sterile mutation is a very common phenomenon in nature, at least male sterile mutants have been found in 617 species of 43 families and 162 genera. Genetically, plant male sterility is divided into three categories: nuclear male sterility, cytoplasmic male sterility and nuclear-cytoplasmic interaction male sterility: 1) Nuclear male sterility is produced by nuclear gene mutations, and there are dominant mutations and recessive mutations , There are sporophytic gene mutations and gametophytic gene mutations. Dominant mutations and gametophytic gene mutations can only be inherited through female gametes, recessive mutations can be inherited through both female gametes and male gametes, and follow Mendel's laws....

AI Technical Summary

Problems solved by technology

However, the cytoplasmic male sterile lines that have been used in maize have some defects: First, because the cytoplasmic male sterile lines need specific restorer genes to restore fertility, the utilization rate of germplasm resources is very low, which limits the efficiency of breeding of excellent varieties Secondly, the fertility of some sterile lines is unstable, and the fertility can be restored under certain conditions, which affects the purity of the hybrid; finally, due to the single cytoplasmic genotype, it causes a large outbreak of corn spot disease, which directly leads to cytoplasmic male sterility technology. qiut the market

The maize MS8 gene has 9 exons, encoding a β-1,3 galactosyltransferase protein, which may be related to lipid synthesis, but its exact substrate has not been found; MS8 loss of function affects the maize anther epidermis Differentiate from the mucous layer, leading to male sterility; in addition to anthers, MS8 is also expressed in a small amount in other tissues, but these tissues have no mutant phenotype in ms8 mutants (Wang et al., 2013, Maize Malesterile 8 (Ms8), a putative β-1,3-galactosyltransferase, modulates cell division, expansion, and differentiation during early maize anther development. PlantReprod, 26:329–338)

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