Culturing method for dominant nitrosation bacterial community

A technology of dominant flora and culture method, applied in biochemical equipment and methods, adding compounds to stimulate growth, microorganisms, etc., can solve the problems of difficult and stable control of DO concentration, long time, and ineffective control, and achieve long-term The effect of stabilizing nitrosation ability, strong bacterial tolerance, and shortening the culture time

Active Publication Date: 2017-05-31
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

However, in practical engineering applications, it is difficult to stably control the DO concentration within the required low concentration range. Once the DO concentration is high, the short-cut nitrification will transform into the full-range nitrification
CN1785843A discloses a method for short-range nitrification of wastewater with low C / N ratio and high concentration of ammonia nitrogen. The method uses the nitrification granular sludge cultivated by anaerobic granular sludge as an inoculum, and gradually increases the concentration of ammonia nitrogen in the substrate to realize it. Although oxygen granular sludge has the advantage of fast cultivation speed, in the subsequent aerobic short-cut nitrification process, there are still shortcomings in the transformation from short-cut nitrification to full-range nitrification.
CN102311918A discloses a method for cultivating nitrosative-dominant bacterial flora, which only relies on external conditions for enrichment and cultivation, and the time is relatively long
[0007] At present, short-cut nitrification uses factors such as low dissolved oxygen (DO), high temperature, high pH, ​​and inhibitory factors to control or screen the dominant nitrosative flora. Due to changes in conditions, the phenomenon of conversion from short-cut nitrification to full-range nitrification cannot be effectively controlled.

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  • Culturing method for dominant nitrosation bacterial community

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Embodiment 1

[0032] The first stage: the enrichment of nitrifying bacteria, the initial ammonia nitrogen concentration of the culture solution is 150mg / L, and NaHCO is used during the enrichment process 3 The solution adjusts the pH value. Culture conditions: temperature is 24℃, pH is 6.0-7.5, SV30 is 15%-20%, DO is 4mg / L. One cycle per day, water intake time is 20 minutes, aeration is 23 hours, natural sedimentation is 30 minutes, and the supernatant is removed. Then add the same volume of the enriched culture medium as the supernatant, and add the microbial growth promoter A-I at the concentration of 20 mg / L of the promoter in the sewage every time the culture medium is replaced. Follow this process to cycle operation, using GB7479 distillation The titration method detects the ammonia nitrogen concentration in the water. After the ammonia nitrogen is not detected, increase the ammonia nitrogen concentration pre-added to the culture solution by 100 mg / L. After 15 days, a mixed flora with a...

Embodiment 2

[0036] The first stage: the enrichment of nitrifying bacteria. The initial ammonia nitrogen concentration of the culture solution is 150mg / L. During the enrichment process, NaHCO is used 3 The solution adjusts the pH value. Culture conditions: temperature is 24℃; pH is 6.0-7.5; SV is 15%-20%; DO is 4mg / L. One cycle per day, water intake time is 20 minutes, aeration is 23 hours, natural sedimentation is 30 minutes, and the supernatant is removed. Then add the same volume of the enriched culture medium as the supernatant, and add the microbial growth promoter A-II according to the concentration of the promoter in the sewage 25mg / L each time the culture medium is replaced, and the cycle operation is performed according to this process, using GB7479 distillation The titration method detects the concentration of ammonia nitrogen in the water. After the ammonia nitrogen is not detected, increase the concentration of ammonia nitrogen pre-added to the culture solution by 100 mg / L. Afte...

Embodiment 3

[0040] The first stage: the enrichment of nitrifying bacteria. The initial ammonia nitrogen concentration of the culture solution is 150mg / L. During the enrichment process, NaHCO is used 3 The solution adjusts the pH value. Culture conditions: temperature is 24℃; pH is 6.0-7.5; SV is 15%-20%; DO is 4mg / L. One cycle per day, water intake time is 20 minutes, aeration is 23 hours, natural sedimentation is 30 minutes, and the supernatant is removed. Then add the same volume of the enriched culture medium as the supernatant. Each time the culture medium is replaced, the microbial growth promoter A-Ⅲ must be added according to the concentration of the promoter in the sewage 25mg / L, and the cycle operation is carried out according to this process, using GB 7479 Distillation titration method detects the ammonia nitrogen concentration in the water. After the ammonia nitrogen is not detected, increase the ammonia nitrogen concentration pre-added to the culture solution by 100 mg / L. After...

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Abstract

The invention relates to a culturing method for a dominant nitrosation bacterial community. The culturing method comprises the following three culturing stages: stage 1, enriching a mixed bacterial community of nitrosomas and nitromonas, wherein a microbial growth promoter A is used in the process of enrichment; stage 2, carrying out nitromonas elutriation by using a microbial growth promoter D and a high-temperature culture and normal-temperature culture alternate method so as to gradually improve the dominance of nitrosomas and beginning third-stage culture when a nitrosation rate is greater than 50%; and stage 3, reducing the content of dissolved oxygen or increasing a pH value, carrying out further nitromonas elutriation and stability domestication of nitrosomas by using the microbial growth promoter D and terminating culture when the nitrosation rate is stabilized and is 65% or more so as to obtain the dominant nitrosation bacterial community. The culturing method provided by the invention overcomes the problems of instability of partial nitrification in practical application, can shorten the starting time of nitrifying process, rapidly changes the process of a nitrifying reaction, broadens the application scope of partial nitrification and provides guarantee for actual application of a partial nitrification process to real projects.

Description

Technical field [0001] The invention belongs to the technical field of sewage biological treatment, and specifically relates to a method for culturing a nitrosation dominant bacterial group. Background technique [0002] The biological denitrification of wastewater mainly relies on the combined action of nitrifying bacteria and denitrifying bacteria in the activated sludge to finally convert various forms of nitrogen in the sewage into gaseous nitrogen and escape from the water. From the perspective of nitrification process, ammonia nitrogen (NH 3 -N) is oxidized to nitrite nitrogen (NO 2 -N) and nitrate nitrogen (NO 3 -N) are two different reactions completed by two separate types of bacteria, which should be separable. From the perspective of denitrification process, NO 2 -N and NO 3 -N can be used as the final electron acceptor. Therefore, the entire biological denitrification process can pass NH 3 -N is converted to NO 2 -N then converted to N 2 Way to complete. [0003] Shor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/00
Inventor 高会杰孙丹凤郭志华赵胜楠
Owner CHINA PETROLEUM & CHEM CORP
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