Yarn for cell culture scaffold and fabric containing the same

A technology of cell culture and yarn, which is applied in the direction of cell culture support/coating, specific-purpose bioreactor/fermenter, tissue culture, etc., which can solve the problems of low cell survival rate, weakened mechanical strength of scaffold, and scaffold shape Deformation and other problems, to achieve the effect of improving cell proliferation and survival, improving cell proliferation rate and survival rate, and ensuring mechanical strength

Active Publication Date: 2021-10-22
AMOGREENTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is a problem in the scaffolds for cell culture or tissue engineering developed so far, that is, since the cells are not cultured into a structure similar to that in the body, and the survival rate of the cells is not high, the cells cultured in this way cannot Suitable for use as in vitro experimental models or cells for transplantation
In addition, in the case of preparing scaffolds for cell culture or tissue engineering using nanofibers suitable for cell size, there may be problems in cell culture due to weak mechanical properties.
[0006] Furthermore, when a degradable compound selected to omit an additional removal operation is applied to a scaffold after transplanting the scaffold into a living body, the shape of the scaffold may be deformed or collapsed during cell culture due to its weak mechanical properties. question
[0007] Furthermore, a conventional degradable compound is a component that is easily decomposed by water, so it is decomposed by the solvent contained in the culture solution put into the cell culture, so there is a problem of further weakening the mechanical strength of the scaffold

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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  • Yarn for cell culture scaffold and fabric containing the same
  • Yarn for cell culture scaffold and fabric containing the same
  • Yarn for cell culture scaffold and fabric containing the same

Examples

Experimental program
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Embodiment approach

[0046] Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, so that those skilled in the art of the present invention can easily implement them. The present invention can be realized in many different forms, and is not limited to the embodiments described here. In order to clarify the present invention, parts irrelevant to the description are omitted from the drawings, and the same reference numerals are assigned to the same or similar structural elements throughout the specification.

[0047] refer to figure 1 and figure 2 According to an embodiment of the present invention, the yarn for a cell culture scaffold includes a slit yarn 200 that cuts and compresses a nanofibrous web 100 including support fibers 220 at a predetermined width.

[0048] The aforementioned nanofibrous web 100 may have a three-dimensional network structure formed by at least one supporting fiber 220 .

[0049] Specifically, a thre...

Embodiment 1

[0104] First, to prepare a spinning solution, 12 g of polyvinylidene fluoride (Arkema, Kynar 761) as a fiber-forming component was dissolved in a 70:30 weight ratio at 80°C using a magnetic bar. dimethylacetamide and acetone in a mixed solvent of 88 g for 6 hours to prepare a mixed solution. Utilize the electrospinning device to electrospin the above-mentioned prepared spinning solution, as the electrospinning conditions, the applied voltage is 25KV, the distance between the current collector and the spinning port is 25cm, and the discharge volume is 0.05ml / hole, at RH 65 Under an environment of 30° C., electrospinning was performed to obtain a nanofibrous web formed of supporting fibers having the diameter distribution shown in Table 1 below. The obtained nanofibrous web was calendered twice at a temperature of 130°C and a pressure of 4kPa, such as Image 6 As shown in part (b), a three-dimensional network structure with a basis weight of 10 g / m 2 , a compressed nanofibrous...

Embodiment 2～12

[0107] Prepared in the same manner as in Example 1, but by adjusting the basis weight / thickness of the nanofibrous web before compression and the strength during compression to make the compressed nanofibers have the basis weight and thickness as shown in Table 3 below, thus obtaining the following The slitting yarns shown in Table 3 were described.

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Abstract

The invention provides a yarn for a cell culture support. The yarn for a cell culture scaffold according to an embodiment of the present invention includes a slit yarn obtained by cutting a compressed nanofiber web into a predetermined width. Thus, by realizing a microenvironment suitable for attachment, migration, proliferation, and differentiation of cultured cells, the survival rate of cells can be improved and cells can be cultured three-dimensionally. Also, the scaffold according to the present invention has sufficient mechanical strength to prevent the scaffold from collapsing or the like during the cultivation of cells, thereby allowing cells to proliferate stably. Further, the scaffold according to the present invention can realize air pores of various sizes by using slitting yarns formed of compressed nanofibrous nets as yarns, thereby improving cell proliferation and survival rate.

Description

technical field [0001] The present invention relates to a cell culture scaffold, and in more detail, to a yarn for a cell culture scaffold with improved mechanical properties and suitable for three-dimensional culture of cells and a fabric containing the same. Background technique [0002] Recently, with the expansion of the utilization of cultured cells for disease treatment, interest and research on cell culture are increasing. Cell culture is a technology that takes cells from an organism and cultures them in vitro, and differentiates the cultured cells into various body tissues such as skin, organs, nerves, etc., and then transplants them into the human body, or uses the cells before differentiation The state is transplanted into the human body, and the growth and differentiation are carried out at the same time, so that it can be used for the treatment of various diseases. [0003] The field related to this cell culture is tissue engineering, a multidisciplinary scienc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D02J3/02D02G3/44A61L27/14A61L27/18A61L27/54A61L27/38D01F8/00C12N5/00
CPCA61L27/18A61L27/38A61L27/54D01F8/00C12M21/08C12M25/14A61L2400/12D02G3/448D02G3/06A61L27/14C12N5/0068D02J3/02A61L2300/604C12N2533/30D10B2509/00A61L27/16D02G3/02D02G3/045D02G3/38
Inventor 徐寅踊张仙虎金灿李承勋具松熙
Owner AMOGREENTECH CO LTD
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