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Method for detecting newly increased detection components in traditional Chinese medicinal Tang Herb for treating AIDS

A determination method and technology of Tangcao Tablets are applied in the field of determination of newly detected components in Chinese medicine Tangcao Tablets, which can solve the problems of cumbersome operation, uncontrollable other components, and unfavorable detection of large batches of samples, so as to improve product quality, and the determination method is stable and reliable. , the effect of large application value

Active Publication Date: 2017-05-24
SHANGHAI HUNDREDS HERBAL PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent 200910048798.6 discloses a quality standard and detection method of Tang Herbal Tablets, a traditional Chinese medicine for treating AIDS. Microscopic features are used to identify the components of scorpion in Tang Herbal Tablet samples; TLC is used to identify geranium, pomegranate peel, Glutinous rice root, licorice, berberine, bupleurum, scutellaria baicalensis, ginkgo leaf and other components; quantitative determination of chlorogenic acid and glucoside in honeysuckle and safflower yellow pigment in safflower in samples of Tangcao tablets by HPLC A content, astragaloside IV content of Radix Astragalus, but the assay methods are carried out separately, so the operation is more cumbersome, unfavorable for the detection of large batches of samples, and other components can not be controlled
However, there is no detection of Myrobalan in the detection of the existing traditional Chinese medicine Tang Herb Tablets. Therefore, further improvement is needed to increase the determination method of related components.

Method used

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  • Method for detecting newly increased detection components in traditional Chinese medicinal Tang Herb for treating AIDS
  • Method for detecting newly increased detection components in traditional Chinese medicinal Tang Herb for treating AIDS
  • Method for detecting newly increased detection components in traditional Chinese medicinal Tang Herb for treating AIDS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Identification of Hedyotis diffusa in Tangcao Tablets: Take Tangcao Tablet Sample 1, Sample 2, and Sample 3 (all produced by Shanghai Baisuihang Pharmaceutical Co., Ltd., commercially available products) each 10 pieces (each piece weighs 0.4g) , grind finely, add 50ml of water, sonicate for 30min, centrifuge, take the supernatant, add 50ml of chloroform (all reagents used are commercially available), extract once, collect the chloroform layer, evaporate to dryness, add 1.5ml of methanol to dissolve the residue As the test product. In addition, get Hedyotis diffusa reference medicinal material (purchased from China Institute for the Control of Biological Products) 0.5g, add water 20ml, heat and reflux for 1.5 hours, filter, add water to the filtrate to 50ml, from adding chloroform 50ml together for the test According to the thin-layer chromatography (Chinese Pharmacopoeia 2010 edition one VIB) test, draw each 10 μ l of the above-mentioned two kinds of solutions, point res...

Embodiment 2

[0040] Embodiment 2: the identification of Myrobalan in Tang Herbal Tablets: (Tang Herbal Tablets sample is the same as Example 1)

[0041] Get 1 g of myrobalan reference medicinal material (purchased from China Institute for the Control of Biological Products), add 20 ml of water, heat and reflux for 1.5 hours, filter, add water to the filtrate to 50 ml, and supply under the identification item of Hedyotis diffusa from adding 50 ml of chloroform. The test sample is operated to make a control medicinal material solution. According to thin-layer chromatography (Chinese Pharmacopoeia 2010 edition one VIB) test, draw each 10 μ l of the need testing solution under contrast medical material solution and embodiment 1 Hedyotis diffusa identification item, point respectively on the same silica gel G thin-layer plate, Use the lower layer of dichloromethane-glacial acetic acid-water (15:5:0.5) as a developing agent, develop, take out, dry in the air, spray with 10% sulfuric acid ethanol...

Embodiment 3

[0043] Example 3 Determination of gallic acid, chlorogenic acid, liquiritin, and berberine II in Tangcao tablets:

[0044] Take an appropriate amount of Tang Herbal Tablet samples (both are produced by Shanghai Baisuihang Pharmaceutical Co., Ltd., commercially available products), grind finely, take 1g, accurately weigh, put in a stoppered Erlenmeyer flask, accurately add 50% methanol 50ml, seal Stopper, weighed, ultrasonically treated for 30 minutes, taken out, placed at room temperature (25±2°C), weighed again, made up for the lost weight with methanol, shaken well, filtered, and it was the test solution, gallic acid , chlorogenic acid, liquiritin, and berberine II reference substance (purchased from China Institute for the Control of Biological Products):

[0045] Precisely weigh the appropriate amount of gallic acid reference substance, chlorogenic acid reference substance, liquiritin reference substance, and berberine Ⅱ reference substance, and add methanol to make each m...

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Abstract

The invention provides a method for detecting newly increased detection components in a traditional Chinese medicinal Tang Herb for treating AIDS. The method for detecting newly increased detection components comprises the following steps: 1, discriminating Hedyotis diffusa and Medicine Terminalia Fruit components in the Tang Herb through adopting thin-layer chromatography; and 2, simultaneously detecting gallic acid, chlorogenic acid, liquiritin and picroside II through gradient elution of a mobile phase under same high performance liquid chromatographic conditions. The content of gallic acid in every tablet of Tang Herb is not less than 0.9 mg, the content of chlorogenic acid in every tablet of the Tang Herb is not less than 0.5 mg, the content of liquiritin in every tablet of the Tang Herb is not less than 0.25 mg, and the content of the picroside II in every tablet of the Tang Herb is not less than 0.45 mg. Full-inspection projects confirm that the newly increased detection component detection method can be added to an original detection method in order to further improve the detection kind and the detection method of a medicine and facilitate the batch production determination of the traditional Chinese medicine Tang Herb and the improvement of the product quality. The detection method provided by the invention has the advantages of good stability and reliability, high specificity and large application values.

Description

technical field [0001] The invention relates to a traditional Chinese medicine compound preparation, in particular to a method for measuring newly added detection components of Tang Herbal Tablets, a traditional Chinese medicine for treating AIDS. Background technique [0002] A large number of experimental and clinical studies on the use of traditional Chinese medicine in the treatment of AIDS at home and abroad have found that the treatment of traditional Chinese medicine in the treatment of AIDS can enhance and stabilize the immune function of the body; And prolong life etc. all have bigger effect. [0003] The traditional Chinese medicine Tang Herbal Tablet consists of geranium, astragalus, solanum nigrum, honeysuckle, kapok, myrobalan, hedyotis diffusa, pomegranate peel, glutinous rice root, water chestnut, melon peel, bupleurum, coriander, licorice, and spathula , safflower, ginkgo biloba, purslane, berberine and scorpion 20 traditional Chinese medicine raw materials....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/90
Inventor 杨莉娅李倩王拂尘邵宝平张超楠安遵华吴卫东
Owner SHANGHAI HUNDREDS HERBAL PHARMA
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