Preparation method of high-efficient decay-promoting agent
A decay-promoting agent and high-efficiency technology, which is applied in the field of preparation of high-efficiency decay-promoting agents, can solve the problems of slow degradation of straw, low purity of strains, low activity of strains, etc., and achieve accelerated decomposition speed, high activity, and shortened cycle Effect
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preparation example Construction
[0025] Experimental materials - preparation of culture medium:
[0026] Nutrient Agar medium: Pepton 5 g, Beef extract 30 g, NaCl 5g, Agar 15 g, add dH 2O to 1000 mL, pH: 7.0-7.2;
[0027] LB medium: Tryptone 10 g, Yeast extract 5 g, NaCl 10 g, add dH 2 O to 1000 mL, pH=7.0;
[0028] MS agar medium: 20 g of agar, 20 g of mannitol, 20 g of soybean powder, dilute to 1000 mL with deionized water, adjust the pH to 7.2 with 5 M NaOH, and sterilize twice under high temperature and high pressure;
[0029] PDA medium: 200g potato, add 1000ml water, boil for half an hour, filter with gauze, add 10-20g glucose and 17-20g agar, sterilize by autoclaving (121°C) for 20 minutes.
Embodiment 1
[0031] (1) Activation and purification of strains and expanded culture on a liquid shaker:
[0032] (1) Inoculate the fungi composed of Saccharomyces cerevisiae, Trichoderma viride, Aspergillus oryzae, and Rhizopus oryzae at a ratio of 25:15:25:15 to PDA medium, place them in a constant temperature incubator at 28°C, and cultivate Until the mycelium is abundant or spores are produced; pick the mycelia or spores of the fungus and inoculate them into the prepared fungal liquid medium for liquid shaker culture, the culture temperature is 28°C, and the shaker culture speed is 200 rpm;
[0033] (2) Inoculate actinomycetes composed of Streptomyces griseus and Streptomyces luteus at a ratio of 45:45 on MS agar medium for streak culture, place in a constant temperature incubator at 30°C, and cultivate to a single colony or spore production; pick single colonies or spores of actinomycetes and inoculate them into the prepared actinomycetes liquid medium for liquid shaker culture, the cu...
Embodiment 2
[0041] (1) Activation and purification of strains and expanded culture on a liquid shaker:
[0042] (1) Inoculate the fungi composed of Saccharomyces cerevisiae, Trichoderma viride, Aspergillus oryzae, and Rhizopus oryzae at a ratio of 30:20:30:20 on the PDA medium, place them in a constant temperature incubator at 28°C, and cultivate Until the mycelium is abundant or spores are produced; pick the mycelia or spores of the fungus and inoculate them into the prepared fungal liquid medium for liquid shaker culture, the culture temperature is 28°C, and the shaker culture speed is 200 rpm;
[0043] (2) Inoculate actinomycetes composed of Streptomyces griseus and Streptomyces luteus at a ratio of 50:50 on MS agar medium for streak culture, place in a constant temperature incubator at 30°C, and cultivate to a single colony or spore production; pick single colonies or spores of actinomycetes and inoculate them into the prepared actinomycetes liquid medium for liquid shaker culture, th...
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