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Preparation method and application of novel sweet Gynostemma pentaphyllum Makino. sweetener

A technology of Gynostemma pentaphyllum and sweetener, which is applied in the fields of separation, purification, preparation and extraction of sweetin, which can solve the problems of low molecular purity, incompletely clarified structure, and absence of Gynostemma pentaphyllum sweetin, so as to achieve pure taste and long aftertaste , the effect of low sweetness threshold

Active Publication Date: 2017-05-24
黄志萍 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two methods are a small amount of extraction in the laboratory, and the molecular purity is not high, and the structure has not been fully clarified
[0005] There is currently no relevant Gynostemma pentaphyllum sweetener patent literature

Method used

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  • Preparation method and application of novel sweet Gynostemma pentaphyllum Makino. sweetener
  • Preparation method and application of novel sweet Gynostemma pentaphyllum Makino. sweetener
  • Preparation method and application of novel sweet Gynostemma pentaphyllum Makino. sweetener

Examples

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example 1

[0031] 1. Select 100g of sweet Gynostemma pentaphylla tea leaves, remove chlorophyll, fat and saponin with an organic solvent in a Soxhlet extractor, and take the residue in a ventilated place to remove the solvent.

[0032] 2. Add 500 mL of distilled water to the Gynostemma pentaphylla treated in step 1, heat it at 70°C-90°C, stir and extract for 0.5h-2h, and repeat the extraction twice. The supernatants were combined by centrifugal filtration and concentrated in vacuo with a rotary distiller to 200 mL.

[0033] 3. Add ammonia water to the concentrated solution in step 2 to adjust the pH of the solution to 8 ~ 9, then add 3mL 30% H 2 o 2 1~3 days for decolorization.

[0034] 4. Use the Sevage method to deproteinize, that is, add 20mL of chloroform / n-butanol mixed solvent with a volume ratio of 5 / 1, stir the mixture with a stirrer for 30 min, take the upper aqueous solution after centrifugation, and separate to remove the junction of the aqueous layer and the solvent layer ...

Embodiment 2

[0042] 1. Select 100g of sweet Gynostemma pentaphyllum tea leaves, add 500mL of distilled water, keep warm at 70°C-80°C, stir and extract for 0.5h-2h, repeat the extraction twice. The supernatants were combined by centrifugal filtration and concentrated in vacuo with a rotary distiller to 200 mL.

[0043] 2. Use a dialysis bag with a molecular weight cut-off range of 500-1000 to dialyze in distilled water for 2 to 4 days, change the water at least once a day, take the dialysate outside the dialysis bag, and use a rotary distiller at a temperature below 70°C and a vacuum of 0.005 Concentrate in vacuum at ~0.05MPa.

[0044] 3. Dialyze the solution in step 2 in distilled water with a molecular weight 100-500 molecular weight dialysis bag, take the solution in the dialysis bag, and concentrate in vacuum with a rotary distiller under the same conditions as above.

[0045] 4. Freeze-dry the above-mentioned concentrated solution in a freeze-drying machine with a vacuum degree of 0.0...

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Abstract

A novel sweetener extracted from sweet Gynostemma pentaphyllum Makino. has a main structure of ethyl-6-glucuronate-1,4-alpha-D-galactose, and is a natural non-carbohydrate sweetener. A preparation method of the novel sweetener comprises the following steps: selecting sweet Gynostemma pentaphyllum Makino. tea, removing chlorophylls, fats and saponins through an organic solvent, carrying out heat insulation distilled water extraction at 70-100 DEG C for 0.5-2 h, decolorizing obtained extract with H2O2, removing proteins through a Sevage technology, and dialyzing the obtained extract in distilled water through using a dialysis bag with the molecular weight cutoff range of 500-2000 in order to remove most polysaccharide and protein impurities; dialyzing the obtained extract in distilled water through using a dialysis bag with the molecular weight cutoff of 100-500 to remove a small amount of salts and the organic solvent; or directly carrying out water extraction without the organic solvent, and removing impurities through a dialysis technology adopting dialysis bags with different molecular weight cutoffs; and drying the finally obtained extract by using a refrigerating drying machine to obtain a sweetener sample. The sweet Gynostemma pentaphyllum Makino. sweetener has high purity, is a natural novel sweetener, has the sweetness 300-1200 times that of sucrose, and does not insulin metabolism.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to the structural characterization and application of a preparation method for extracting, separating and purifying sweetener from sweet Gynostemma pentaphyllum. Background technique [0002] Gynostemma is also known as paradise grass, gospel grass, super ginseng, cypress bottom, rooting everywhere, aescin, five-leaf ginseng and seven-leaf ginseng, etc. Latin name: Gynostemma pentaphyllum (Thunb.) Makino Cucurbitaceae, Gynostemma herbaceous quality Climbing plants, stems slender, branched, with longitudinal ribs and grooves, glabrous or sparsely pubescent. Japan calls it Ganman tea. Gynostemma likes a humid and mild climate, and it mostly grows wild in shaded places such as forests and streams, and is a perennial climbing herb. In China, it is mainly distributed in Shaanxi Pingli, Gansu Kangxian, Hunan, Hubei, Yunnan, Guangxi and other provinces. It ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L27/30
Inventor 黄志萍刘金龙曹明保聂海英
Owner 黄志萍
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