A kind of breeding method of Agaricus bisporus strain suitable for standardized factory

Abstract

The invention discloses an agaricus bisporus strain breeding method applicable to a standardized plant. An agaricus bisporus strain is taken as a mother strain for breeding, a fruiting experiment is performed, stock seeds are prepared, triticale is selected as a raw material, the raw material is soaked in a solution with the pH value ranging from 11.0 to 13.0 and stirred to adjust the pH value to range from 7.0 to 9.0, half of a bag of the raw material is quantitively packaged with a sterile bag, a lantern ring and a breather plug are used for sealing, sterilization is performed at the high temperature of 120-130 DEG C under the pressure of 0.10-0.20 MPa for 2-3 h, sterile inoculation is performed after cooling, and the agaricus bisporus strain is cultured at the constant temperature. By means of the method, high-quality, pure and high-yield agaricus bisporus cultivars can be bred in batches, the breeding cycle is shortened greatly, the pollution probability is reduced, the general breeding time of the cultivars lasts 15 days to 18 days, the water content of bred strain grains is consistent, the biofilm growing phenomenon is avoided, the qualification rate can reach 96%, and the method has broad practicability in strain breeding fields.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation technology, and more specifically, the invention relates to the technical field of a method for breeding Agaricus bisporus strains suitable for standardized factories. Background technique [0002] Edible fungi are the future healthy food for human beings, and the artificial cultivation of edible fungi has become a new branch of modern agriculture. In terms of agricultural ecological cycle, the cultivation of edible fungi occupies a very important position. At present, the industrialization of edible fungi is developing vigorously across the country. The main species of edible fungi is the grass rot fungus Agaricus bisporus (Agaricus bisporus), which has high protein content and is fresh and delicious. leading product. [0003] At present, the traditional mode of cultivating Agaricus bisporus by artificial soil method is transitioning to the mode of semi-factory and industrial...

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Problems solved by technology

[0004] Aiming at the deficiencies and existing problems of the current state of the art, especially the low quality and unstable properties of traditional workshop-style Agaricus bisporus strains, which affect the bottleneck problem of the development of the Agaricus bisporus industry in China, in order to solve the above problems, the present invention provides a kind of Compared with the existing technology, the method of breeding Agaricus bisporus strains in standardized factories can breed high-quality, pure, and high-yielding Agaricus bisporus cultivars in large quantities, and the cultivation period of the traditional cultivation method is 30-45 days. The cultivation cycle reduces the chance of pollution. The general cultivation time of cultivars is 15 days to 18 days. There is no need to cook triticale, which saves energy. 96%, due to the selection of second-level species for breeding third-level species, no matter whether grains are used as solid strains or liquid strains, they can be evenly mixed or sprayed into the triticale medium in a tenth-level purification environment. There are many growth points and fast germination. The inoculation time of each particle of the same batch of substrates is roughly the same, so the time point of initial growth is consistent, the age of the bacteria is the same, the age of the bacteria is short, the vitality of the bacteria is strong, and the distribution of mycelia is uniform and standardized. , has a wide range of practicability in the field of strain cultivation

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