Sturgeon compound powder, sturgeon bone wine and its application
A technology of compound powder and sturgeon, which is applied in the field of sturgeon bone wine and its application, compound powder of sturgeon, to achieve the effects of improving the body's immunity, enriching nutrition and protecting the liver
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Embodiment 1
[0020] A kind of sturgeon composite powder, is prepared according to the following steps:
[0021] a. Wash the slaughtered sturgeon, freeze-dry the sturgeon meat and sturgeon cartilage until the water content is less than 1%, and pulverize the freeze-dried sturgeon meat and sturgeon cartilage into 2500-mesh sturgeon superfine powder;
[0022] b. Grinding the sturgeon superfine powder into 100 nm sturgeon nanopowder;
[0023] c. Add 0.05M phosphate buffer solution of pH 7 to 8 and compound enzyme to the sturgeon nanopowder, and perform enzymatic hydrolysis for 30 h; the mass ratio of the sturgeon nanopowder to the phosphate buffer solution is 1:5, and the compound enzyme The addition amount is 15 activity units: 1 gram of sturgeon nanopowder, and the compound enzyme is mixed with subtilisin, papain, and alkaline protease according to the activity unit ratio of 3:3:4;
[0024] d. Take the supernatant by centrifugation, separate it with an ultrafiltration membrane separator with...
Embodiment 2
[0029] a. Wash the slaughtered sturgeon, freeze-dry the sturgeon meat and sturgeon cartilage until the water content is less than 1%, and pulverize the freeze-dried sturgeon meat and sturgeon cartilage into 1000-mesh sturgeon superfine powder;
[0030] b. Grinding the sturgeon superfine powder into 70nm sturgeon nanopowder;
[0031] c. Add 0.1M phosphate buffer solution of pH 7 to 8 and a compound enzyme to the sturgeon nanopowder, and perform enzymatic hydrolysis for 12 hours; the mass ratio of the sturgeon nanopowder to the phosphate buffer solution is 1:1, and the compound enzyme is added The amount is 25 activity units: 1 gram of sturgeon nanopowder, and the compound enzyme is mixed with subtilisin, papain, and alkaline protease according to the activity unit ratio of 3:3:4;
[0032] d. Take the supernatant by centrifugation, separate it with an ultrafiltration membrane separator with a molecular weight cut-off of 10,000 Da or less, collect the liquid that passes through t...
Embodiment 3
[0034] a. Wash the slaughtered sturgeon, freeze-dry the sturgeon meat and sturgeon cartilage until the water content is less than 1%, and crush the freeze-dried sturgeon meat and sturgeon cartilage into 4000-mesh sturgeon superfine powder;
[0035] b. Grinding the sturgeon superfine powder into 200 nm sturgeon nanopowder;
[0036] c. Add 0.02M phosphate buffer solution of pH 7 to 8 and compound enzyme to the sturgeon nanopowder, and enzymatically hydrolyze for 48 h; the mass ratio of the sturgeon nanopowder to the phosphate buffer solution is 1:10, and the compound enzyme The addition amount is 10 activity units: 1 gram of sturgeon nanopowder, and the compound enzyme is mixed with subtilisin, papain and alkaline protease according to the activity unit ratio of 3:3:4;
[0037] d. Take the supernatant by centrifugation, separate it with an ultrafiltration membrane separator with a molecular weight cut-off of 10,000 Da or less, collect the liquid that passes through the ultrafilt...
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