Detection method for antibiotic residue in milk

Abstract

The invention discloses a detection method for antibiotic residue in milk. The detection method comprises the steps of (1) taking and adding 2 to 4mL of milk into a centrifuge tube, adding 0.3 to 0.5g of demulsifying agent, ultrasonically oscillating for 15 to 30min, then adding 10 to 20mL of ethyl acetate, uniformly dispersing, adding 10 to 30mL of phosphate buffered solution, ultrasonically extracting for 10 to 20min, centrifuging, then transferring 5mL of liquid supernatant, adding 5mL of phosphate buffered solution, uniformly mixing, then dissolving in a sodium hydroxide solution or a potassium hydroxide solution, adding methylbenzene for extraction and taking an upper solution as a standard solution; (2) transferring the standard solution in the step (1) to a solid phase extraction column, leaching by using 5mL of water and 5mL of methanol aqueous solution in sequence, discarding effluent, decompressing and pumping, then eluting with 5mL of methanol and collecting eluant. Blowing to nearly dry with nitrogen at 50 DEG C water bath, adding 5mL of acetonitrile water solution, mixing by a turbine mixer, and obtaining filter liquor through a microfiltration membrane for being detected; (3) adding the filter liquor into a gas chromatograph for detection. The detection method provided by the invention can effectively improve detection result accuracy and provides guarantee for food safety.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method for antibiotic residues in milk. Background technique [0002] Milk is rich in all nutrients needed by the human body except dietary fiber, and its high nutrition is irreplaceable by other foods. Fresh milk and dairy products have become an important part of people's (especially the elderly and children's) life food. In recent years, with the rapid development of my country's animal husbandry industry, milk production has increased significantly. In order to prevent the occurrence of diseases and treat diseases such as mastitis, farmers will add feed additives containing a certain proportion of antibiotics to cattle feed and drinking water. However, this will inevitably result in antibiotic residues in milk. If you drink milk containing antibiotics for a long time, the antibiotics in the milk can enter the human body through the food chain, which is undoubtedly equivalent...

AI Technical Summary

Problems solved by technology

Among them, solid-phase extraction technology is the most widely used, but in the pretreatment of antibiotic residue detection in milk, solid-phase extraction technology generally has complex sample processing process, more exposure to toxic reagents, large consumption of consumables, and the need for additional nitrogen blowing. , poor reproducibility and the inability to realize fully automatic online connection

Especially drugs such as tetracyclines, because they combine with proteins or minerals to form a bound state, so it is more difficult to detect residual antibiotics in milk