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Periplaneta americana immunomodulating peptide and preparation method and medical application thereof

A technology of immune regulation and Periplaneta americana, applied in the field of biomedicine, to achieve the effect of increasing the total number of spleen lymphocytes and the proportion of NK cells, increasing the total number of white blood cells, and convenient source of raw materials

Inactive Publication Date: 2017-05-10
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The results of the novelty search show that there have been no relevant reports on the immunomodulatory peptide involved in the present invention obtained from the medicinal insect Periplaneta americana in the past

Method used

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  • Periplaneta americana immunomodulating peptide and preparation method and medical application thereof
  • Periplaneta americana immunomodulating peptide and preparation method and medical application thereof
  • Periplaneta americana immunomodulating peptide and preparation method and medical application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Example 1 : Preparation of Periplaneta americana immunomodulatory peptide (sample HFDT1)

[0021] Main instruments and reagents: Acetonitrile (Fisher Company, chromatographically pure, batch number: 134752), methanol (Amethyst Chemical Company, chromatographically pure, batch number: 135586), trifluoroacetic acid (Tianjin Kemiou Chemical Reagent Co., Ltd., chromatographically pure, batch number: 20130424); AJY-2001-U ultrapure water instrument (Aikepu International Co., Ltd.), high performance liquid chromatography (Agilent1100, including: automatic sampler, DAD detector, chromatography workstation), Seppak small column (Waters company ), centrifuge, ultrafiltration membrane (Amicon Ultra Milipore, MW=3KDa), vortex mixer.

[0022] 1.1 Preparation of Periplaneta americana alcohol extract

[0023] Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 75% ethanol, soak and extract at room temperature, extract 3 times, each time for 24 hours. The extrac...

Embodiment 2

[0035] Example 2 : Effect of Periplaneta americana Immunomodulatory Peptide on Immunity of MFC Tumor-bearing Mice

[0036] 2.1 Materials and Instruments

[0037] 2.1.1 Experimental animals and cell lines: 6-8 weeks old SPF grade BALB / c mice, about 20 grams each, purchased from Hunan Slack Jingda Experimental Animal Co., Ltd. (SYXK (Xiang) 2013-0004); Mouse gastric cancer cells (MFC cell line) were purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences.

[0038] 2.1.2 Experimental instruments: blood cell analyzer (BC-2800Vet, Mindray), low-speed centrifuge (LC-4012, HKUST Innovation), flow cytometer (FACS Calibur, BD Company of the United States), electronic balance (MJ-500, Japan Chyo), CO 2 Incubator (MCO-18AIC, Japan SANYO), cell mesh screen (Cell Strainer, BD Falcon).

[0039] 2.1.3 Main drugs and reagents: artificially synthesized short peptide HFDT1 according to the amino acid sequence provided in Example 1 (verified, its molecular weight is 1...

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Abstract

The invention relates to a periplaneta americana immunomodulating peptide and a preparation method and medical application thereof. Specifically, the invention relates to an immunomodulating peptide which is extracted from periplaneta americana and has the effects of preventing and treating tumor diseases and improving the immunity function of an organism. The polypeptide substance is a single chain polypeptide of which the amino acid sequence is arginine-arginine-asparagine-leucine-leucine-alanine-alanine-serine-leucine-leucine-asparagine- aspartate, and is prepared by extracting fresh or dry periplaneta americana in water or an organic solvent and a buffer solution and refining through a membrane separation method and an HPLC (High Performance Liquid Chromatography) technology. The immunomodulating peptide has remarkable antitumor activity and immunomodulating effect, and can be used to make medicaments for preventing and treating tumor diseases and / or modulating immunity, daily chemical products or medical devices.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, in particular, the present invention relates to a polypeptide substance having the effect of enhancing human immune function, its preparation method and its medical application. The polypeptide substance is an active substance extracted and isolated from the medicinal insect Periplaneta americana. Background technique [0002] The American cockroach (Periplaneta americana Linn.), commonly known as cockroach, is an insect belonging to the genus Periplaneta in the subclass Insecta Pterata. Periplaneta americana was first used as medicine in "Shen Nong's Materia Medica", which listed it as a middle-grade product, saying that "taste: salty, cold; treatment: blood stasis, hard cold and heat, broken accumulation, closed throat, internal cold and no seeds" ( Sun Xingyan et al., "Shen Nong's Materia Medica", The Commercial Press, 1955:90). "Cockroach" is its common name first seen in "Compe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/36C07K1/34C07K1/16A61K38/10A61P35/00A61P37/02
Inventor 张成桂罗廷顺白丽常旭陈壮志赵微巫秀美刘光明约阿施·史托克希特
Owner DALI UNIV
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