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Fermentation culture method for isaria fumosorosea

A technology for spores and fermentation culture, which can be applied to the methods of using spores, methods based on microorganisms, biochemical equipment and methods, etc., and can solve the problems of intolerant storage of propagules, contamination of miscellaneous bacteria, complicated methods, etc. , to achieve the effect of low cost, short production cycle and high purity

Active Publication Date: 2017-04-26
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The economical, fast and large-scale production of I. fumigatus is the prerequisite for its large-scale use in biological control practice. The propagules produced by liquid fermentation alone are not resistant to storage. At present, the fermentation method of I. fumigatus is relatively complicated. The cost is high, and the phenomenon of bacterial contamination is easy to occur during the cultivation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Liquid fermentation stage: based on 100ml of liquid culture medium, inoculate 3.6ml of spore suspension of I. fumigatus (each ml contains 1.0×10 7 spores) were cultivated for 3 days at 26°C with a rotation speed of 125r / min to obtain a mycelium liquid;

[0017] 2) Solid fermentation stage: inoculate the mycelial liquid in the solid medium, the volume (ml) of the mycelial liquid accounts for 10% of the weight (g) of the solid medium, place it at 25° C. and cultivate it for 15 days before harvesting.

[0018] Described liquid medium containing carbon source (glucose) is 25g / L, and nitrogen source (yeast powder) is 25g / L, and containing trace element (copper sulfate, zinc sulfate, magnesium sulfate, weight is 1:1:1) is 150mg / L, containing 1g / L of basic salt (dipotassium hydrogen phosphate, potassium chloride, weight 1:1), pH 6. Autoclave with moist heat for 30 minutes, cool down and set aside.

[0019] The solid culture medium is 300 g of corn flour, 700 g of water is...

Embodiment 2

[0022] 1) Liquid fermentation stage: based on 100ml of liquid culture medium, inoculate 1ml of spore suspension of I. fumigatus, and cultivate for 1 day at 20°C with a rotation speed of 200r / min to obtain a mycelium liquid;

[0023] 2) Solid fermentation stage: inoculate the mycelia liquid in the solid medium, the volume (ml) of the mycelia liquid accounts for 4% of the weight (g) of the solid medium, place the culture at 15°C for 18 days and then harvest.

[0024] Described liquid culture medium contains carbon source (sucrose, cornflour extract, weight is 3:1) is 10g / L, and nitrogen source (peptone, NH 4 Cl, weight is 4:1) is 5g / L, contains trace element (copper sulfate) is 50mg / L, contains basic salt (sodium chloride) is 0.5g / L, pH value is 8.

[0025] The solid medium is 600 g of peanut cake powder, added with 400 g of water, mixed evenly, and the pH value is 8.

[0026] The harvested spore powder was dried and measured, 6.86g of spore powder per 500g of dry substrate, 9....

Embodiment 3

[0028] 1) Liquid fermentation stage: based on 100ml of liquid culture medium, inoculate 8ml of spore suspension of I. fumigatus, and cultivate for 2 days at 29°C with a rotation speed of 100r / min to obtain a mycelia liquid;

[0029] 2) Solid fermentation stage: inoculate the mycelia liquid in the solid medium, the volume (ml) of the mycelia liquid accounts for 12% of the weight (g) of the solid medium, place the mycelia liquid at 35° C. for 8 days and then harvest.

[0030] Described liquid culture medium contains carbon source (maltose, wheat bran extract, weight is 5:1) is 30g / L, and nitrogen source (peptone, yeast powder, weight is 1:1) is 25g / L, contains trace element ( Zinc sulfate) is 250mg / L, containing basic salt (sodium chloride, potassium chloride, the weight is 2:1) is 1.5g / L, and the pH value is 4.

[0031] The solid medium is 100g each of wheat bran and soybean meal, added with 800g of water, mixed evenly, and the pH value is 8.

[0032] The harvested spore powde...

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PUM

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Abstract

The invention discloses a fermentation culture method for isaria fumosorosea. The method comprises two stages, i.e. liquid fermentation and solid fermentation. Each liter of liquid culture medium contains 10 to 30 grams of a carbon source, 5 to 25 grams of a nitrogen source, 50 to 250 milligrams of a trace element and 0.5 to 1.5 grams of base salt, and the pH value is 4 to 8. A solid culture medium is corn flour, rice, peanut meal, bran and soybean meal. The produced isaria fumosaroseus is very high in spore output, purity and spore viability, the whole fermentation culture period is about 20 days, and the method has the advantages of short production period, low cost, high efficiency and the like, and is applicable to large-scale production of the isaria fumosorosea.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting and cultivating I. fumigatus. Background technique [0002] Isaria fumosaroseus is a kind of pathogenic fungus that widely infects a variety of insects. It has a strong infection effect on citrus psyllids and is an excellent strain for biological control of citrus psyllids. The economical, fast and large-scale production of I. fumigatus is the prerequisite for its large-scale use in biological control practice. The propagules produced by liquid fermentation alone are not resistant to storage. At present, the fermentation method of I. fumigatus is relatively complicated. The cost is high, and the phenomenon of bacterial contamination is easy to occur during the cultivation process. Therefore, research and development of a new high-efficiency, low-cost large-scale production medium and a culture method that is easy to operate and not easy t...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 张宏宇刘亚茹王珊珊
Owner HUAZHONG AGRI UNIV
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