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A kind of sericin composite hydrogel and preparation method thereof

A technology of composite hydrogel and sericin, which is applied in the field of sericin composite hydrogel and its preparation, and polymer materials, can solve the problems of unsolved problems such as the controlled release speed and service life of the hydrogel, and achieves the Good mechanical properties, quick gelation effect

Active Publication Date: 2019-07-26
BEIJING SINOCELL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the controlled release rate and service life of hydrogels are still unsolved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of sericin composite hydrogel, comprising the following steps:

[0028] (1) Dialysis bag pretreatment: Soak the dialysis bag in the mixed aqueous solution of sodium carbonate, EDTA and SDS for 12 hours, then boil for 12 minutes, the pH of the mixed aqueous solution is 8.0, and the molar ratio of sodium carbonate, EDTA and SDS is 1:1:1,; use double distilled water to rinse the dialysis bag to neutral, then boil it in EDTA with a pH of 8.0 and a molar concentration of 1 mmol / L for 3 minutes, and store it at room temperature;

[0029] (2) Primary selection of sericin: clean the cocoons with double distilled water, soak them in 1.5mmol / L sodium bicarbonate aqueous solution for 1 hour, and dry them at room temperature;

[0030] (3) Sericin pretreatment: put the sericin treated in step (2) in 0.03mmol / L sodium carbonate aqueous solution, then transfer it to an autoclave, and sterilize at 121°C for 50 minutes. The mass ratio of gelatin and sodium carbonat...

Embodiment 2

[0035]A preparation method of sericin composite hydrogel, comprising the following steps:

[0036] (1) Dialysis bag pretreatment: Soak the dialysis bag in the mixed aqueous solution of sodium carbonate, EDTA and SDS for 19 hours, then boil for 18 minutes, the pH of the mixed aqueous solution is 8.2, and the molar ratio of sodium carbonate, EDTA and SDS is 7:2:3,; use double-distilled water to rinse the dialysis bag to neutral, then boil it in EDTA with a pH of 8.0 and a molar concentration of 1.6mmol / L for 6 minutes, and store it at room temperature;

[0037] (2) Primary selection of sericin: clean the cocoons with double distilled water, soak them in 2.2mmol / L sodium bicarbonate aqueous solution for 2.5 hours, and dry them at room temperature;

[0038] (3) Sericin pretreatment: put the sericin treated in step (2) in 0.05mmol / L sodium carbonate aqueous solution, then transfer it to an autoclave, and sterilize at 121°C for 65 minutes. The mass ratio of gelatin and sodium carbo...

Embodiment 3

[0043] A preparation method of sericin composite hydrogel, comprising the following steps:

[0044] (1) Dialysis bag pretreatment: Soak the dialysis bag in the mixed aqueous solution of sodium carbonate, EDTA and SDS for 24 hours, then boil for 20 minutes, the pH of the mixed aqueous solution is 8.6, and the molar ratio of sodium carbonate, EDTA and SDS is 8: 3:5,; use double distilled water to rinse the dialysis bag to neutrality, then boil it in EDTA with a pH of 8.0 and a molar concentration of 2.5mmol / L for 8 minutes, and store it at room temperature;

[0045] (2) Primary selection of sericin: clean the cocoons with double distilled water, soak them in 3mmol / L sodium bicarbonate aqueous solution for 4 hours, and dry them at room temperature;

[0046] (3) Sericin pretreatment: put the sericin treated in step (2) in 0.06mmol / L sodium carbonate aqueous solution, then transfer to an autoclave, sterilize at 121°C for 80 minutes, and the silk The mass ratio of gelatin and sodiu...

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Abstract

The invention discloses a sericin protein composite hydrogel and a preparing method thereof. The preparing method comprises dialysis bag pretreatment, sericin protein primary election, sericin protein pretreatment, sericin protein dialysis, composite hydrogel precursor preparation and product finishing process. The sericin protein composite hydrogel prepared from the method can quickly gelatinize, and has an injectable property; the sericin protein composite hydrogel prepared from the method has a porous structure, and is not only a space for proliferation and survival of cells provided, but also a microenvironment for maintenance and release of bioactive factor is provided; the sericin protein composite hydrogel prepared from the method has a good mechanical property.

Description

technical field [0001] The invention belongs to the field of tissue engineering and relates to a polymer material, in particular to a sericin composite hydrogel and a preparation method thereof. Background technique [0002] Since the first appearance of "hydrogel" in scientific literature at the end of the 19th century, the research on hydrogel has roughly gone through four stages. The application of hydrogels is mainly focused on ophthalmology and drug delivery research. The second generation of hydrogels mainly occurred in the 1970s, and the research on hydrogels changed from relatively simple water-swellable macromolecular networks to the field of environment-responsive hydrogels. Environmental response factors include pH, temperature, concentration of biomolecules, and the like. These environmental factors can be used to cause specific events, such as the formation of a gel or the release of a drug. The third generation of hydrogels mainly began in the mid-1990s. Phy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/54A61L27/56A61L27/50A61L27/20A61L27/22
CPCA61L27/20A61L27/227A61L27/52A61L27/54A61L27/56A61L2400/06C08L89/00C08L5/08
Inventor 韩之海周世容
Owner BEIJING SINOCELL CO LTD
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