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Culturing method of selenium-enriched cordyceps militaris

A technology for Cordyceps militaris and worms, applied in the field of selenium-enriched Cordyceps militaris cultivation, can solve the problems of difficulty in improving the nutrition and medicinal value of Cordyceps militaris, difficulty in absorbing Cordyceps militaris, and low selenium content.

Active Publication Date: 2017-03-29
XIAMEN YUANZUN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the source of selenium in most of the technical solutions for cultivating selenium-enriched Cordyceps militaris is mostly selenium-containing fertilizer nutrient solution, and most of the selenium fertilizer nutrient solution uses sodium selenite as the source of selenium element, using sodium selenite as the selenium element The source of Cordyceps militaris is difficult to absorb, resulting in very little selenium content in the fruiting body of Cordyceps militaris, it is difficult to improve the nutritional and medicinal value of Cordyceps militaris, and the residue of too much inorganic selenium has brought about the utilization of Cordyceps militaris culture medium. Negative Effects

Method used

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Examples

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preparation example Construction

[0076] The preparation method of amino acid nutrient mother liquor is: take glycine, threonine, valine, leucine, isoleucine, phenylalanine, serine, proline, hydroxyproline, cysteine, color Amino acid, methionine, lysine and glutamic acid, add distilled water to make up to 1000ml; store at 4°C for later use.

[0077] PDA solid medium: 20g potato extract powder, 20g glucose, 20g agar, distilled water to 1000mL; sterilize by high pressure steam at 121℃ for 20min.

[0078] The preparation method of seed strengthening medium: take selenium-enriched yeast powder, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, amino acid nutrient mother solution, vitamin B 1 and vitamin B 2 , add distilled water to 100ml to make nutrient solution A; mix nutrient solution A with millet evenly, and steam for 30 minutes to obtain seed strengthening medium; sterilize by autoclaving at 121°C for 25 minutes. The seed strengthening medium A, the seed strengthening medium B and the se...

Embodiment 1

[0095] Embodiment 1, the breeding of Cordyceps militaris FY1421

[0096] Using Cordyceps militaris strain 50383 as the starting strain, after atmospheric room temperature plasma (ARTP) mutagenesis treatment, a strain of Cordyceps militaris was screened and named as Cordyceps militaris FY1421 (Cordyceps militaris FY1421). Cordyceps militaris FY1421 (Cordyceps militaris FY1421) was deposited in the China Center for Type Culture Collection (CCTCC for short; address: Wuhan, China, Wuhan University; Zip code: 430072) on April 21, 2016, and the preservation number is CCTCC NO: M2016220. Cordyceps militaris FY1421 (Cordyceps militaris FY1421) CCTCC No: M2016220 is referred to as Cordyceps militaris FY1421 for short.

Embodiment 2

[0097] Embodiment 2, the acquisition of Cordyceps militaris fruiting body

[0098] 1. Inoculate the Cordyceps militaris strain 50383 into the PDA solid slant medium, and culture in the dark at 25°C for 6 days.

[0099] 2. Use sterile physiological saline to elute the spores on the PDA solid slant medium cultivated in step 1, transfer to a new PDA solid slant medium, and culture in the incubator at 25°C for 5 days in the dark to obtain activation. of Cordyceps militaris strains.

[0100] 3. The spores of the activated Cordyceps militaris bacterial strain of step 2 are eluted with 10ml sterile physiological saline, and transferred to the eggplant bottle in which 100g seed strengthening medium is housed (the initial concentration of the Cordyceps militaris bacterial strain in the culture system is 10 4 Individual / gram culture), in the incubator 25 ℃ of dark light static cultures for 5 days, obtain the strengthened seed culture (spore concentration is 10 12 per gram of culture)....

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Abstract

The invention discloses a culturing method of selenium-enriched cordyceps militaris, and more specifically provides a method used for preparing cordyceps militaris sporocarp products. The method comprises following steps: a1, a seed enriched medium is used for culturing cordyceps militaris strains so as to obtain a seed enriched cultured product; a2, a liquid seed medium is inoculated with the seed enriched cultured product obtained via step a1 so as to obtain liquid seed via culturing; and a3, a sporocarp selenium-enriched culture medium is inoculated with the liquid seed for culturing so as to obtain cordyceps militaris sporocarp. According to the method, selenium-enriched yeast powder is taken as an organic nitrogen source in cordyceps militaris sporocarp culturing, and is also used for providing selenium, so that selenium utilization rate and absorptivity are increased, and the selenium content of sporophore is increased greatly; and in addition, selenium in the selenium-enriched yeast powder exists in the form of organic selenium, so that the selenium-enriched yeast powder is a safe and effective selenium source of selenium-enriched sporophore; the method can be popularized in the industry; and economic and social benefits are high.

Description

technical field [0001] The invention relates to a method for cultivating selenium-enriched Cordyceps militaris. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, belongs to the kingdom Fungi, and taxonomically belongs to the phylum Fungi, Ascomycota, Sclerotinia, Coccomycetes, Ergotaceae, Cordyceps genus, Cordyceps militaris is widely distributed and grows It is fast, easy to cultivate, contains a variety of active ingredients, and has a variety of physiological functions on the human body. [0003] Modern research shows that the active substances of Cordyceps militaris generally belong to nucleosides, sugar alcohols, polysaccharides, amino acids, enzymes and mineral elements. The identified functional factors mainly include cordycepin, adenosine, mannitol, cordyceps polysaccharide, superoxide dismutase (SOD) and so on. Among them, cordycepin is the most important one, which plays an important role in clinical medic...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G1/04C12R1/645
CPCA01G18/00C12N1/14
Inventor 骆梅香黄钦耿刘晓红陈瑞琛吴松刚
Owner XIAMEN YUANZUN BIOLOGICAL ENG CO LTD
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