Temperature response type core cross-linked star polymer with glycoprotein enriching effect and preparation method thereof

Abstract

The invention discloses a temperature response type core cross-linked star polymer with a glycoprotein enriching effect and a preparation method thereof. According to the synthesized temperature response type core cross-linked star polymer with the glycoprotein enriching effect, an outer arm is prepared from a recognizing unit, i.e., phenylboronic acid, which plays a role in recognizing glycoproteins, and can be specifically bound to the glycoproteins, so as to complete a glycoprotein trapping process; the cross-linked core is prepared from a temperature response type polymer and can subject to a swelling 'dissolving'-precipitating change along with temperature changes, so as to complete a process of separating the glycoproteins from a mixed solution. The method has the advantages that the influence caused by interfacial mass transfer in an enriching process is eliminated, the capacity of enrichment is increased greatly, and the time for incubation is shortened; meanwhile, compared with the other methods, recognizing units are fixed by an arm precedence method, so that the operation is relatively simple, the fixed amount is high, and thus, the capacity of enrichment is further increased. In addition, the method can achieve the effective and rapid separation of the glycoproteins through simple centrifugation or filtration, so that the requirements on modern bioseparation are met.

Description

[0001] Technical field: [0002] The invention relates to a temperature-responsive nuclear cross-linked star polymer with enrichment effect on glycoproteins and a preparation method thereof, belonging to the fields of material science and biological separation engineering. [0003] Background technique: [0004] Glycoproteins are an important class of post-translational proteins in which oligosaccharide chains are covalently linked to proteins. Glycosylated proteins participate in many physiological activities in living organisms, such as cell recognition, signal transmission, immune response, etc. At the same time, a large number of studies have shown that the abnormality of sugar chain structure is related to the occurrence of many diseases, so some glycoproteins can be used as biomarkers of certain diseases. Therefore, the research on the structure of glycoprotein and the detection of abnormal glycoprotein are of great significance. However, for actual samples, the low abu...

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Problems solved by technology

However, glycoproteins are biological macromolecules with high molecular weight and complex conformation. Although the introduction of recognition units on temperature-responsive polymers can realize the capture of glycoproteins, it is difficult to extract glycoproteins from the mixed solution in terms of single molecular chains. seperate

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