Hydrogel system for trapping and targeted release of circulating tumor cells

A tumor cell and hydrogel technology, applied in the field of biological hydrogel rice materials, can solve problems such as the lack of reliable solutions, and achieve the effect of fixed-point release and good light response

Active Publication Date: 2021-11-16
济南国科医工科技发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Photosensitive hydrogel systems have the potential to be applied to the capture and separation of circulating tumor cells, but there is a lack of reliable solutions

Method used

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  • Hydrogel system for trapping and targeted release of circulating tumor cells
  • Hydrogel system for trapping and targeted release of circulating tumor cells
  • Hydrogel system for trapping and targeted release of circulating tumor cells

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Embodiment 1

[0037] This embodiment provides a hydrogel system for the capture and fixed-point release of circulating tumor cells. The hydrogel system is prepared by the following method:

[0038] 1) Preparation of Ti 3 C2 T x two-dimensional material

[0039] First prepare 6M hydrochloric acid (HCl) aqueous solution for later use, measure 10ml of HCl solution in a beaker, weigh 0.666g of lithium fluoride (LiF) and dissolve it in the HCl solution, place it on a stirring table and stir for 40 minutes; Remove it and pour it into a single-necked flask, place it in an oil bath at 35°C and preheat it with stirring, weigh 1g MAX-Ti 3 AlC 2 Dissolve in the flask solution, heat and stir for 24 hours to generate Ti 3 C 2 T x Material.

[0040] 2) Utilize Ti 3 C 2 T x Preparation of Ti from two-dimensional materials 3 C 2 T x monolayer nanosheet suspension

[0041] With the Ti obtained in step 1) 3 C 2 T x The two-dimensional material (black solution) was centrifuged three times in ...

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Abstract

The invention discloses a hydrogel system for capturing and fixed-point release of circulating tumor cells, which is characterized in that the hydrogel system is prepared by the following method: 1) preparing Ti 3 C 2 T x Two-dimensional materials; 2) using Ti 3 C 2 T x Preparation of Ti from two-dimensional materials 3 C 2 T x monolayer nanosheet suspension; 3) using Ti 3 C 2 T x The monolayer nanosheet suspension and gelatin are used to prepare a hydrogel film, and then the surface of the hydrogel film is modified by carboxylation, and the hydrogel system is obtained after coupling the EpCAM antibody. The hydrogel system of the present invention has better photoresponse, thermal stability and cell adhesion, and can specifically capture circulating tumor cells by modifying the surface of the hydrogel membrane and coupling antibodies; The hydrogel system can rapidly heat up under the irradiation of laser with specific wavelength, so that the targeted release of circulating tumor cells can be realized.

Description

technical field [0001] The invention relates to the field of biological hydrogel rice materials, in particular to a hydrogel system used for the capture and fixed-point release of circulating tumor cells. Background technique [0002] Circulating rare cells has important implications for materials research and clinical applications. Among them, circulating tumor cells are the biomarkers of tumor "liquid biopsy"; non-invasive prenatal diagnosis can be realized by identifying and extracting circulating fetal nucleated red blood cells; others, such as circulating stem cells and endothelial cells, also have their own potential indicators in clinical diagnosis application. However, in the context of rich blood cells, it is challenging to characterize and isolate circulating rare cells with extremely low abundance. How to effectively capture and isolate circulating rare cells is a key research goal in the fields of biology and medicine. [0003] In recent years, due to its adjus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J5/18C08J7/12C08L89/00C08K3/14C12N5/09
CPCC08J5/18C08J7/12C08J2389/00C08K3/14C08K2201/011C12N5/0693C12N2509/00
Inventor 董文飞尤倩楠梅茜葛明锋从瑛哥常智敏李力宁珊珊
Owner 济南国科医工科技发展有限公司
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