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Chitosan affine heptapeptide and screening method and application thereof

A screening method, chitosan technology, applied in the field of chitosan affinity heptapeptide and its application, to achieve the effect of improving stability

Active Publication Date: 2017-03-22
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the flexibility and porosity of chitosan materials, the method of obtaining chitosan affinity peptides and affinity peptides through screening of phage display peptide libraries has not been reported.

Method used

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  • Chitosan affine heptapeptide and screening method and application thereof
  • Chitosan affine heptapeptide and screening method and application thereof
  • Chitosan affine heptapeptide and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0021] Binding phage panning

[0022] Preparation of chitosan nanoparticles coated with mesoporous silicon

[0023] Synthesis of carboxylated mesoporous silicon nanoparticles: first weigh 0.8g of perfluorobutylsulfonyl fluoride FC-4 and 0.65g of mixed block polyether F-127, add them to 40ml of 0.02M HCl solution in turn, and then add them to 250ml Condensate and reflux in a Erlenmeyer flask. When the perfluorobutylsulfonyl fluoride FC-4 is completely dissolved, add 2.2g of tetraethoxysilane (TEOS) into the mixture and stir vigorously at 30°C for 20h. When the mixture becomes When it is in the state of white milky cloud, raise the temperature to 100°C and continue stirring vigorously for 24h. The obtained product was cooled at room temperature, and the product was washed three times with a mixture of ultrapure water and pure ethanol at a speed of 12,000 rpm. After the obtained solid was dried in a vacuum oven, it was dried in a tube furnace at 550 ° C. Calcined for 3 hours to...

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PUM

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Abstract

The invention discloses chitosan affine heptapeptide. The amino acid sequence of the chitosan affine heptapeptide is shown as any of SEQ ID NO.1-SEQ ID NO.6. Screening includes the steps that nanoparticles wrapped by chitosan are prepared through the ion gelation method, then the nanoparticles are mixed with a phage displayed peptide library, the peptide library is screened through centrifugal separation, and finally a phage clone obtained through screening is subjected to ELISA identification and sequence analysis. The provided short peptide has high affinity and can be bound to the surface of a chitosan material, surface properties of the mesoporous silicon nanoparticles wrapped by chitosan can be changed, and accordingly the application range is widened. More materials, methods and means are provided for developing nano materials, especially nanoparticle medicine carriers, with chitosan as the basis.

Description

technical field [0001] The invention belongs to the field of nano-biological materials, and more specifically relates to chitosan affinity heptapeptide and its application. Background technique [0002] Chitosan (chitosan) is the second largest natural organic polymer compound in nature after cellulose. It is the only alkaline polysaccharide that exists in large quantities. It has good biocompatibility, degradability, high charge density and Antibacterial properties, which have unique physical and chemical properties and biological functions, are usually obtained by deacetylation of chitin. Chitosan is widely used in the field of biomedicine, and the way most biomolecules interact with chitosan is to rely on non-specific interactions. If there is a way to control the characteristics of chitosan, such as assisting chitosan to make nano Particles, improve the recognition and specificity of chitosan and other biomolecules, which will greatly improve the function of chitosan. ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C40B30/04C08B37/08
CPCC07K7/06C08B37/003C40B30/04
Inventor 王宜冰于勇姚文基王平
Owner EAST CHINA UNIV OF SCI & TECH
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