Transgenic vector system capable of promoting cell transplantation and gene expression and applications of transgenic vector system

Abstract

The invention discloses a transgenic vector system capable of promoting cell transplantation and gene expression. The transgenic vector system comprises a screening gene system, a target gene expression system, and a vector used for bearing the two systems, wherein the screening gene system is an expression system of a gene capable of promoting cell division or cell survival or a mutant of the gene, or a silencing system of a gene capable of inhibiting division or promoting cell death. Compared with the prior art, the system has the advantages that firstly, the limitation that whether the target gene has the growth vigor or not is eliminated, secondly, the screening occurs on both the stem cell level and the dedifferentiating cell level, and therefore, the gene modification cells can be stably maintained all the life or the number of the gene modification cells can be increased; thirdly, medicine screening is not needed, and therefore, the influences of the toxicity of the medicines are avoided; moreover, the mutant only promoting the cell survival but not promoting the cell mutation is adopted, and therefore, the risk of causing cancer is avoided. Therefore, the system has great application values in gene and cell therapy and the manufacturing of animal bioreactors or animal models based on adult (stem) cells.

Description

technical field [0001] The invention relates to a transgene carrier system, especially a carrier system capable of promoting cell transplantation and gene expression. Background technique [0002] Gene therapy or the establishment of adult (stem) cell-based animal bioreactors or animal models require stable target gene expression levels and stable target gene expression positive cell numbers in recipient patients or animals. However, in reality, it generally occurs that the efficiency of expressing the target gene in a single cell decreases and the proportion of cells expressing the target gene decreases. An approach to address the above deficiencies is to perform in vivo screening. There are currently three types of in vivo screening methods: 1) The target gene has the ability to make the host cell continue to divide and promote survival, so that in vivo screening occurs naturally without any screening pressure, but the characteristics of this target gene are not common, ...

AI Technical Summary

Problems solved by technology

There are currently three types of in vivo screening methods: 1) The target gene has the ability to make the host cell continue to divide and promote survival, so that in vivo screening occurs naturally without any screening pressure, but the characteristics of this target gene are not common, That is, not all target genes have the ability to make the host cell continue to divide and promote survival; 2) In addition to the target gene, a screening gene is introduced at the same time, which enables the host cell to have drug-dependent ability to promote survival (so-called The cell growth switch cell growth switches), but this method shows that the screening only occurs at the level of short-term survival cells in large animals, so that it cannot maintain enough cells expressing the target gene for a long time, thus limiting its use; 3) except In addition to the target gene, a screening gene that resists screening pressure (usually toxic drugs) is introduced at the same time, so that the drug only eliminates non-genetically modified cells but not genetically modified cells, but this system hinders its application due to toxic drugs

4) When a gene with a growth advantage is used as a screening gene, it has the possibility of causing tumorigenesis

So far, no in vivo screening system that can overcome the above defects has been seen

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