Streptomyces griseorubens beta-1, 4-glucosidase and coding gene and application thereof
A technology of Streptomyces grisea and glucosidase, which is applied in the field of genetic engineering and modern enzyme engineering, can solve the problems of low content and low activity, achieve high-efficiency expression, convenient purification, and ensure biological activity
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[0037] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention, not to limit the protection scope of the present invention. Improvements and adjustments made by those skilled in the art according to the present invention in practical applications still belong to the protection scope of the present invention.
[0038] 1. Genome extraction and sequencing of Streptomyces grisea
[0039] Inoculate the spore suspension of Streptomyces grisea into LB liquid medium, culture at 37°C and 150rpm for 3 days, then collect 2.0mL of the bacterial liquid, centrifuge at 12000rpm for 2min, discard the supernatant, collect the bacterial precipitate, and then Add 180 μL lysozyme (20 mg / mL) and 20 μL EDTA solution (0.5M, pH 8.0), treat at 37°C for 45 minutes, add 4 μL RNase A (100 mg / mL), shake and mix for 15 seconds, leave at room temperature for 5 minutes, and then fol...
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