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A method of constructing sema 4D/Heparin microenvironment on the surface of cardiovascular implant materials

An implant material, cardiovascular technology, applied in pharmaceutical formulations, prostheses, surgery, etc., can solve the problem of non-discovery, and achieve the effect of delaying half-life

Active Publication Date: 2019-07-12
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Heparin (Heparin) is often used as an anticoagulant clinically, mainly for the treatment of thrombosis, thrombophlebitis and thromboembolism; the structure of heparin contains a large number of sulfate groups, and it is the most negatively charged biological substance found so far. Molecule; Sema4D is an anchor protein on the surface of cell membranes, widely present on the surface of platelets, T cells, etc., and its extracellular protein hydrolyzate Sema 4D can promote endothelial cell migration and immune regulation; Sema 4D receptors include plexin B1 and CD72, The former mainly exists on the surface of endothelial cells, while the latter mainly exists in immune cells. The function of Sema 4D is mainly realized by acting on the corresponding receptors; the PI of Sema 4D is 8.5, and it presents a positive charge under neutral pH conditions. Combination of effects; studies have shown that the combination of heparin and various growth factors can protect growth factors, delay the half-life of growth factors, and promote the recognition and combination of growth factors and their receptors; so far, no combination of heparin and Sema 4D combines technologies for cardiovascular materials

Method used

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  • A method of constructing sema 4D/Heparin microenvironment on the surface of cardiovascular implant materials
  • A method of constructing sema 4D/Heparin microenvironment on the surface of cardiovascular implant materials
  • A method of constructing sema 4D/Heparin microenvironment on the surface of cardiovascular implant materials

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Experimental program
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Effect test

Embodiment 1

[0044] A method for constructing a Sema 4D / Heparin microenvironment on the surface of a cardiovascular implant material, comprising the following steps:

[0045] A. Alkali activation

[0046] After the pure titanium is polished and cleaned, it is activated with 1mol / L NaOH for 12 hours. After ultrasonic cleaning with single distilled water, it is immersed in double distilled water. It is reacted at 80°C for 12 hours. After ultrasonic cleaning with single distilled water, it is dried for later use;

[0047] B. Surface amination

[0048] The alkali-activated sample in step A was immersed in 2.5mg / ml polylysine (PLL, MW 150-300KDa) at 4°C for 12h, washed with phosphate buffer and then used;

[0049] C, Preparation of Sema 4D / Heparin complex

[0050] Mix Sema 4D with a concentration of 50ng / ml and Heparin with a concentration of 5mg / ml in equal volumes, and react at 37°C for 1h;

[0051] D. Sema 4D / Heparin fixed

[0052] Soak the sample in step B in the complex prepared in ste...

Embodiment 2

[0054]A method for constructing a Sema 4D / Heparin microenvironment on the surface of a cardiovascular implant material, comprising the following steps:

[0055] A. Alkali activation

[0056] After the pure titanium is polished and cleaned, it is activated with 4mol / L NaOH for 16 hours. After ultrasonic cleaning with single distilled water, it is immersed in double distilled water. It is reacted at 80°C for 12 hours. After ultrasonic cleaning with single distilled water, it is dried for later use;

[0057] B. Surface amination

[0058] The alkali-activated sample in step A was immersed in 2.5mg / ml polylysine (PLL, MW 150-300KDa) at 4°C for 12h, washed with phosphate buffer and then used;

[0059] C, Preparation of Sema 4D / Heparin complex

[0060] Mix Sema 4D with a concentration of 200ng / ml and Heparin with a concentration of 8mg / ml in equal volumes, and react at 37°C for 3h;

[0061] D. Sema 4D / Heparin fixed

[0062] Soak the sample in step B in the complex prepared in ste...

Embodiment 3

[0064] A method for constructing a Sema 4D / Heparin microenvironment on the surface of a cardiovascular implant material, comprising the following steps:

[0065] A. Alkali activation

[0066] After the pure titanium is polished and cleaned, it is activated with 2mol / L NaOH for 10 hours, after ultrasonic cleaning with single distilled water, it is immersed in double distilled water, reacted at 80°C for 12 hours, after ultrasonic cleaning with single distilled water, it is dried for later use;

[0067] B. Surface amination

[0068] The alkali-activated sample in step A was immersed in 2.5mg / ml polylysine (PLL, MW 150-300KDa) at 4°C for 12h, washed with phosphate buffer and then used;

[0069] C, Preparation of Sema 4D / Heparin complex

[0070] Mix Sema 4D with a concentration of 400ng / ml and Heparin with a concentration of 10mg / ml in equal volumes, and react at 37°C for 2h;

[0071] D. Sema 4D / Heparin fixed

[0072] Soak the sample in step B in the complex prepared in step C,...

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Abstract

The invention discloses a method for constructing a Sema 4D / Heparin microenvironment on the surface of a cardiovascular implant material, comprising the following steps: A, alkali activation of the material by using NaOH; B, surface amination of the sample prepared in step A; C . Preparation of Heparin / Sema 4D complex; D. Heparin / Sema 4D complex is fixed on the surface of the sample prepared in step B; the present invention combines Sema 4D and heparin and fixes it on the surface of the material, which can protect Sema 4D and avoid Degraded by protease, it can promote the recognition and binding between Sema 4D and its corresponding receptors to ensure the function of Sema 4D; it can better simulate the process of vascular wound repair in the body, the process is simple, easy to operate, and does not require expensive and complicated equipment, and It is suitable for various complex cardiovascular implants and other surfaces with anticoagulation / promoting endothelial migration requirements.

Description

technical field [0001] The invention relates to an inorganic material surface modification technology, in particular to a method for constructing a Sema 4D / Heparin microenvironment on the surface of a cardiovascular implant material. Background technique [0002] Cardiovascular system disease is one of the important diseases that endanger human health. Artificial implantation of vascular stents or heart valves is an effective way to treat cardiovascular diseases; currently, more than 2 million coronary heart disease patients worldwide need percutaneous coronary angioplasty every year In 2006, more than 100,000 patients received vascular stent implantation in my country; valvular heart disease accounts for about 30% of heart disease patients in China, and more than 100,000 critically ill patients must undergo manual surgery every year. Heart valve replacement. [0003] Titanium and its alloys are widely used in the field of biomedicine due to their good biocompatibility; vascu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/34A61L31/14A61L31/10
CPCA61L27/34A61L27/507A61L31/10A61L31/14A61L2400/18A61L2420/02A61L2420/06C08L89/00C08L5/10
Inventor 陈俊英崔园园周丰曾峥谭建英魏来牟小辉黄楠
Owner SOUTHWEST JIAOTONG UNIV
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