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Building method and application of Sema 4D-VEGF coating

A construction method and coating technology, applied in the field of surface modification of biomedical materials, to achieve the effect without equipment

Active Publication Date: 2017-07-28
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Heparin (Heparin) is often used as an anticoagulant clinically, mainly for the treatment of thrombosis, thrombophlebitis and thromboembolism; the structure of heparin contains a large number of sulfate groups, and it is the most negatively charged biological substance found so far. Molecule; Sema4D is an anchor protein on the surface of cell membranes, widely present on the surface of platelets, T cells, etc., and its extracellular protein hydrolyzate Sema 4D can promote endothelial cell migration and immune regulation; Sema 4D receptors include plexin B1 and CD72, The former mainly exists on the surface of endothelial cells, while the latter mainly exists in immune cells. The function of Sema 4D is mainly realized by acting on the corresponding receptors; a large number of studies have shown that the combination of heparin and various growth factors can protect growth factors. Delay the half-life of growth factors, and promote the recognition and binding between growth factors and their receptors; so far, no technology has been found to use the synergistic effect of Sema 4D on the surface of the cell membrane and VEGF to construct a microenvironment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A. After polishing and cleaning the surface of the base material, use 1-4mg / ml dopamine hydrochloride solution, pH 8.5, soak and react at room temperature for 12-24 hours, then clean; repeat the soaking and cleaning process 2-5 times to form a quinone-based active layer; The base material is made of pure titanium, which is ultrasonically cleaned with pure water for 3 minutes; a layer of quinone-based active layer is formed after soaking and cleaning, and the soaking and cleaning process is repeated 2-5 times to prepare a multi-layer quinone-based active layer to ensure that there are enough active quinones on the surface of the material base, which is beneficial to the fixation of the subsequent amino layer;

[0028] B. The quinone-based active layer in step A is soaked with 2.5mg / ml polylysine at 4°C for 12 hours, and cleaned; the molecular weight of polylysine is 150-300KDa, and the cleaning process is cleaned with phosphate buffer ;

[0029] C. Mix 50-400ng / ml of Se...

Embodiment 2

[0039] A. After polishing and cleaning the surface of the base material, use 1-4mg / ml dopamine hydrochloride solution, pH 7.4; wash after soaking and reacting for 12-24 hours at room temperature; repeat the soaking and cleaning process 2-5 times to form a quinone-based active layer; The base material is made of pure titanium, which is ultrasonically cleaned with pure water for 3 minutes; a layer of quinone-based active layer is formed after soaking and cleaning, and the soaking and cleaning process is repeated 2-5 times to prepare a multi-layer quinone-based active layer to ensure that there are enough active quinones on the surface of the material base, which is beneficial to the fixation of the subsequent amino layer;

[0040] B. The quinone-based active layer in step A is soaked with 2.5mg / ml polylysine at 4°C for 12 hours, and cleaned; the molecular weight of polylysine is 150-300KDa, and the cleaning process is cleaned with phosphate buffer ;

[0041] C. Mix 50-400ng / ml ...

Embodiment 3

[0046]A. After polishing and cleaning the surface of the base material, use 1-4mg / ml dopamine hydrochloride solution, pH 8.5, soak and react at room temperature for 12-24 hours, then clean; repeat the soaking and cleaning process 2-5 times to form a quinone-based active layer; The base material is made of pure titanium, which is ultrasonically cleaned with pure water for 3 minutes; a layer of quinone-based active layer is formed after soaking and cleaning, and the soaking and cleaning process is repeated 2-5 times to prepare a multi-layer quinone-based active layer to ensure that there are enough active quinones on the surface of the material base, which is beneficial to the fixation of the subsequent amino layer;

[0047] B. The quinone-based active layer in step A is soaked with 2.5mg / ml polylysine at 4°C for 12 hours, and cleaned; the molecular weight of polylysine is 150-300KDa, and the cleaning process is cleaned with phosphate buffer ;

[0048] C. Mix 50-400ng / ml of Sem...

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Abstract

The invention discloses a building method and application of a Sema 4D-VEGF coating. The building method includes steps: A, preparing a quinone-based active layer; B, aminating the surface of a substrate material; C, fixing a Sema 4D nanocompound; D, loading a functional factor VEGF. A quick-endothelialization microenvironment under synergism of Sema 4D-VEGF is built, and tissue repair induction and quick endothelialization functions of a cardiovascular material can be realized by biologically modifying the surface of the material through the building method.

Description

technical field [0001] The invention relates to the field of surface modification of biomedical materials, in particular to a construction method and application of a Sema 4D-VEGF coating. Background technique [0002] Cardiovascular system disease is one of the important diseases that endanger human health. Artificial implantation of vascular stents or heart valves is an effective way to treat cardiovascular diseases; currently, more than 2 million coronary heart disease patients worldwide need percutaneous coronary angioplasty every year In 2006, more than 100,000 patients received vascular stent implantation in my country; valvular heart disease accounts for about 30% of heart disease patients in China, and more than 100,000 critically ill patients must undergo manual surgery every year. Heart valve replacement. [0003] Heparin (Heparin) is often used as an anticoagulant clinically, mainly for the treatment of thrombosis, thrombophlebitis and thromboembolism; the structur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/16A61L31/10A61L33/12
CPCA61L31/10A61L31/16A61L33/128A61L2300/252A61L2300/412A61L2300/414A61L2300/606A61L2400/18A61L2420/02A61L2420/06C08L89/00
Inventor 陈俊英崔园园周丰牟小辉彭行溉曾峥魏来许方婷
Owner SOUTHWEST JIAOTONG UNIV
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