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Parachlorella kessleri with tolerance to high pH and culture and applications of parachlorella kessleri

A pseudo-Chlorella, Kjeldahl technology, applied in the field of microbial engineering, can solve the problems of low carbon dioxide fixation efficiency and low solubility, and achieve the effects of good absorption and utilization of carbon dioxide, increased growth rate, and improved fixation efficiency.

Active Publication Date: 2017-03-01
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Pseudochlorella kelvinii selected by the present invention can better absorb and utilize carbon dioxide at a high pH value, grow and reproduce rapidly, and solve the problem that the existing Pseudochlorella kelvinia can only grow under a neutral pH, and the neutral pH Under PH conditions, there are problems of low solubility of carbon dioxide and low efficiency of fixing carbon dioxide

Method used

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  • Parachlorella kessleri with tolerance to high pH and culture and applications of parachlorella kessleri
  • Parachlorella kessleri with tolerance to high pH and culture and applications of parachlorella kessleri
  • Parachlorella kessleri with tolerance to high pH and culture and applications of parachlorella kessleri

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation and Screening of Mutant FSH-Y3 of Chlorella kelsii

[0036] The original Chlorella Kjeldahlii strain of the present invention is a wild Chlorella Kjeldahl isolated from a water sample collected from Hunhe River, Fushun City, Liaoning Province in November 2011, and is numbered FSH-3. . The original Chlorella kessleri strain FSH-3 was subjected to UV mutagenesis and domestication to breed a high pH-tolerant Chlorella kessleri mutant FSH-Y3.

[0037] The method of obtaining the Chlorella kelsii mutant strain FSH-Y3 of the present invention is specifically as follows:

[0038] (1) Prepare the BG11 medium. Divide the prepared BG11 medium into 250mL shake flasks, each with a volume of 100mL, seal it with a breathable membrane, wrap it in kraft paper, and sterilize it at 121℃, 0.1MPa. minute. The composition of the BG11 medium is shown in Table 1 and Table 2.

[0039] Table 1 BG11 medium

[0040]

[0041] *Table 2 Composition of A5+Co solution in Table 1

[0042] ...

Embodiment 2

[0048] Example 2 Comparison of the growth rate of original algae strain and mutagenic algae strain under different pH

[0049] The selected algae strain FSH-Y3's logarithmic growth phase and the original algae species FSH-3's logarithmic growth phase are connected to a shaker bottle containing BG11 medium in a certain proportion, and the initial after inoculation OD 680 The values ​​are the same, both are 0.2. Enter CO from the bottom of the reactor 2 Gas with a content of 5v%, culture for 7 days at different pH values, and determine OD 680 The value is used as an index to measure the growth rate, and the results are shown in Table 3.

[0050] Table 3 Comparison of OD values ​​between original algae strains and mutagenic algae strains under different pH values

[0051]

[0052] It can be seen from Table 3 that the OD value of the mutagenized algae strain does not change significantly with the increase of PH value, while the OD value of the original algae strain drops rapidly, grows ...

Embodiment 3

[0053] Example 3 Comparison of growth effects of FSH-Y3 and FSH-3

[0054] The algae fluids of FSH-Y3 and FSH-3 in the logarithmic growth phase were respectively inoculated in BG11 medium for cultivation. The cultivation was carried out in a column reactor (inner diameter of 40mm and height of 500mm) made in the laboratory. Density adjusted to OD 680 Is 0.2. According to the needs of the test, different levels of CO are combined 2 The gas is then introduced from the bottom of the reactor. During the culture process, the light intensity is 5000 Lux, the culture temperature is 28°C, the pH value is controlled at 11, the light cycle is 24h, the light-dark time ratio is 14:10, and the culture time is 7 days. After the culture is completed, the algal cells are collected at -60 After vacuum freeze-drying to constant weight at ℃, the dry weight of algae flour was measured and the biomass yield was calculated. The results are shown in Table 4.

[0055] Table 4 Different CO 2 Comparison ...

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Abstract

The invention discloses parachlorella kessleri with the tolerance to a high pH and culture and applications of parachlorella kessleri. A parachlorella kessleri mutant strain FSH-Y3 with the tolerance to the high pH is obtained through the method adopting ultraviolet mutagenesis and acclimation screening utilizing step-by-step pH increase, the classification name is Parachlorella Kessleri, and the parachlorella kessleri strain FSH-Y3 is prepared in the China General Microbiological Culture Collection Center (CGMCC) on May 26, 2014, and is assigned with the accession number of CGMCC No.9238. The bred parachlorella kessleri FSH-Y3 can well absorb and utilize carbon dioxide at the high pH, and rapidly grow and reproduce, therefore, the problems that the existing parachlorella kessleri only can grow at the neutral pH, but the solubility of carbon dioxide is low, and the carbon dioxide fixing efficiency is low under the neutral pH condition are solved.

Description

Technical field [0001] The invention belongs to the field of microbial engineering, and specifically relates to a high PH-tolerant Chlorella kessleri and its cultivation application. Background technique [0002] With the development of the world economy, the use and consumption of a large amount of fossil energy has led to energy shortages and environmental degradation, especially CO 2 The greenhouse effect caused by the sharp increase in sulphur is more and more serious. The growth cycle of microalgae is short and the photosynthetic efficiency is high. 2 High fixation efficiency, which can reach more than 10 times that of terrestrial plants under certain conditions, which can not only reduce CO 2 Emissions, while also reducing training costs; in addition to CO 2 In addition, some SOx, NOx and other components in the exhaust gas are also purified along with the metabolism of microalgae, which effectively reduces harmful gas emissions. Therefore, biodiesel produced using microalgae...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N15/01C12N13/00C12P7/64C12R1/89
Inventor 师文静廖莎孙启梅王鹏翔李晓姝樊亚超张霖
Owner CHINA PETROLEUM & CHEM CORP
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