A method to improve the utilization rate of rainbow trout pseudo-male sperm
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A technology of pseudo-male fish and utilization rate, applied in the fields of application, preservation of human or animal body, animal husbandry, etc., can solve the problems of low fertilization rate and eye formation rate of fish eggs, low sperm activation rate, etc., and achieve excellent delayed coagulation. performance and stability, reduce the consumption of fish eggs, and improve the effect of sperm capacitation
Active Publication Date: 2020-01-31
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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[0004] The present invention aims to solve the problems of low sperm activation rate, low fertilization rate and eye formation rate of fish roe after processing the sperm of rainbow trout pseudo-male in the existing method, and provides a method for improving the utilization rate of rainbow trout pseudo-male sperm
Method used
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specific Embodiment approach 1
[0015] Specific implementation mode 1: The method for improving the sperm utilization rate of rainbow trout pseudomale in this embodiment includes the following steps:
[0016] 1. Preparing rainbow trout pseudomale semen preservation solution, the semen preservation solution includes 6~8g / L NaCl, 2~4g / L KCl, 0.2~0.4g / L CaCl 2 ·2H 2 O, 0.2~0.4g / L MgCl 2 ·6H 2 O, 0.1~0.3g / L NaHCO 3 , 4~5g / LC 6 H 5 Na 3 O 7 And distilled water.
[0017] 2. After the semen preservation solution is prepared, adjust the pH value to 9-10, and then place it at 8-12°C for 2-3 hours for use.
[0018] 3. Select high-quality and healthy rainbow trout pseudo-males, dissect the pseudo-male testes, place the pseudo-male testes in a clean and water-free mortar, grind them to obtain a semen tissue mixture, filter the mixture with gauze to obtain Pseudo-male semen, mix the pseudo-male semen and the semen preservation solution in a volume ratio of 1:1 and place it in an oxygen bag. The oxygenation concentration is cont...
specific Embodiment approach 2
[0019] Specific embodiment two: this embodiment is different from specific embodiment one in that: the semen preservation solution in step one includes 7g / L NaCl, 3g / L KCl, 0.3g / L CaCl 2 ·2H 2 O, 0.3g / L MgCl 2 ·6H 2 O, 0.2g / L NaHCO 3 , 4.5g / L C 6 H 5 Na 3 O 7 And distilled water. Others are the same as the first embodiment.
specific Embodiment approach 3
[0020] Specific embodiment three: this embodiment is different from specific embodiment one in that: the semen preservation solution in step one includes 7.6g / L NaCl, 2.98g / L KCl, 0.37g / L CaCl 2 ·2H 2 O, 0.31g / L MgCl 2 ·6H 2 O, 0.21g / LNaHCO 3 , 4.2g / L C 6 H 5 Na 3 O 7 And distilled water. Others are the same as the first embodiment.
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Abstract
The invention discloses a method for increasing the utilization rate of sperms of sex reversal milter of rainbow trout, relates to a method for increasing the utilization rate of sperms of milter, and aims to solve the problems that the sperm activation rate, the fish egg fertility rate and the eyed rate are low after sperms of sex reversal milter of rainbow trout are treated by using a conventional method. The method comprises the following steps: I, preparing a rainbow trout sex reversal milter semen storage liquid; II, after the semen storage liquid is prepared, adjusting the pH value, and leaving to stand at 8-12 DEG C for later use; III, cutting high-quality and healthy rainbow trout so as to obtain a sex reversal milter spermary, putting the sex reversal milter spermary into a clean mortar without water, grinding the sex reversal milter spermary so as to obtain a mixed semen tissue liquid, filtering the mixed semen tissue liquid by using gauze, mixing the sex reversal milter semen with the semen storage liquid, putting into an oxygen bag, storing, and taking out for fish egg fertilization. The activation rate of the sperms of the sex reversal milter can reach more than 90%, and the fertilization rate and the eyed rate of fish eggs are both more than 70%, therefore, waste of the fish eggs can be greatly reduced. The method is applicable to the field of genetic breeding of the rainbow trout.
Description
Technical field [0001] The invention relates to a method for improving the sperm utilization rate of pseudomale fish. Background technique [0002] Rainbow trout farming has been in operation for more than 100 years and is one of the earliest salmon and trout farming species. Genetic improvement can effectively reduce production costs. The use of chromosome manipulation technology, polyploid cultivation, and large-scale production of all female diploid and triploid fertilized eggs is one of its important methods. All-female triploids have incomparable advantages, such as growth advantages and disease resistance brought by long lifespan and sterility at mature stage, which are unmatched by diploid fish. They are sought after by many breeding units and are imported in large quantities from abroad every year. Eggs are currently the main breed of rainbow trout. [0003] Pseudo-male fish is a key technology for preparing full-female triploids. Full-female triploids can be obtained by...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 谷伟王淑梅王炳谦户国徐革锋孙鹏
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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