Method for determination of fat-soluble vitamins by using semi-automatic sample treatment liquid chromatographic technology
A technology for fat-soluble vitamins and sample treatment solutions, which is applied in the field of vitamin detection, can solve problems such as the confusion in the use of vitamin products, and achieve the effects of being suitable for clinical popularization and application, reducing matrix effects, and having strong specificity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] Example 1 Simultaneous determination of multiple fat-soluble vitamins in serum using semi-automatic sample processing liquid chromatography technology
[0042] Proceed as follows:
[0043] (1) Prepare the sample to be tested: place 200ul of serum sample in a semi-automatic liquid-liquid extraction instrument, add an equal volume of frozen ethanol, vortex for 2 minutes, and then add 800ul of frozen 0.4g / L 2,6-di-tert-butyl The n-hexane solution of methyl-4-methylphenol, vortex at 1500rpm for 10 minutes, then centrifuge at 3000rpm for 20 minutes, take the supernatant, and add 800ul of frozen 0.4g / L 2,6-di-tert-butyl to the precipitate after centrifugation The n-hexane solution of -4-methylphenol, vortex at 1500rpm for 10 minutes, then centrifuge at 3000rpm for 20 minutes, combine the supernatants, blow on nitrogen flow until nearly dry, then reconstitute with 100ul frozen ethanol, vortex for 60s, filter through 0.2um Membrane, sample injection analysis. In this example, two ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com