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Composite nanofiber film of structure bionic skin extracellular matrix and producing method and application thereof

A technology for compounding nanofibers and skin cells, applied in the field of biomedical composite materials, can solve problems such as scars on wound surfaces, and achieve the effects of simple and easy preparation method, good biocompatibility and biomechanical strength, and abundant raw material resources.

Inactive Publication Date: 2017-02-15
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nanofibers of the current conventional nanofiber-based skin scaffolds are randomly oriented, which is similar to the disordered arrangement of collagen fibers at the scar formation site, which may lead to the formation of scars on the wound surface

Method used

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  • Composite nanofiber film of structure bionic skin extracellular matrix and producing method and application thereof
  • Composite nanofiber film of structure bionic skin extracellular matrix and producing method and application thereof
  • Composite nanofiber film of structure bionic skin extracellular matrix and producing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Weigh 0.35g PLA and 0.35g type I collagen (the mass ratio of PLA and collagen is 1:1) with an electronic balance, and dissolve them in 5ml hexafluoroisopropanol to obtain a solution with a mass-volume concentration of 14% (m / v). The solution was magnetically stirred at room temperature for 12 hours to dissolve completely, and after standing for 5 minutes, electrospinning was started; the parameters of electrospinning were: electrostatic voltage 24kV, receiving distance 15cm, spinning rate 1.5ml / h, relative humidity 50%. A high-speed rotating drum (2000 rpm) was used as a receiver to obtain a composite nanofiber membrane. Depend on figure 1 It can be seen from the scanning electron microscope image that the fiber orientation of the composite nanofibrous membrane prepared in this embodiment is consistent.

Embodiment 2

[0033] Weigh 0.75g PLGA with an electronic balance and dissolve it in 5ml of dichloromethane to obtain a solution with a mass-to-volume concentration of 15% (m / v). The solution is magnetically stirred at room temperature for 8 hours to dissolve completely, and after standing for 5 minutes, start electrospinning. silk; the parameters of electrospinning are: electrostatic voltage 14kV, receiving distance 6cm, spinning rate 3ml / h, relative humidity 80%. A glass slide sandwiched between two parallel copper strips is used as a receiver, and through the action of an additional electric field, the fibers fall on the glass slide in a parallel orientation to obtain a composite nanofiber film. Depend on figure 2 It can be seen from the scanning electron microscope images that the fiber orientations of the composite nanofiber membranes prepared in this embodiment are parallel to each other.

Embodiment 3

[0035] Weigh 0.525g of PCL and 0.175g of type I collagen (the mass ratio of PCL and collagen is 1:3) with an electronic balance, dissolve them in 5ml of hexafluoroisopropanol, and obtain a solution with a mass-volume concentration of 8% (m / v), The solution was magnetically stirred at room temperature for 12 hours to dissolve completely, and after standing for 5 minutes, electrospinning was started; the parameters of electrospinning were: electrostatic voltage 18kV, receiving distance 25cm, spinning rate 0.5ml / h, relative humidity 20%. A glass slide sandwiched between two parallel copper strips is used as a receiver, and through the action of an additional electric field, the fibers fall on the glass slide in a parallel orientation, and the glass slide is rotated 90 degrees at regular intervals to finally obtain a composite nanofiber film. Depend on image 3 It can be seen from the scanning electron microscope image that the fiber orientation of the composite nanofibrous membra...

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PUM

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Abstract

The invention discloses a composite nanofiber film of a structure bionic skin extracellular matrix and a producing method and application thereof, and belongs to the field of biomedical composite materials. The method includes the following steps: selecting a main ingredient in the skin extracellular matrix as a material; and adopting an electrospinning technique, controlling different technical parameters, using a high-speed rotation roller (at the rotation speed of 500-3000 rpm), parallel plate electrodes horizontally arranged as a receiver, or using a near field electrostatic spinning machine to produce the composite nanofiber film having an ordered structure. The producing method is simple to implement; the produced composite nanofiber film has excellent biocompatibility and biomechanical strength, fibers intersect with each other at a certain angle, and woven basket-shaped structure bionic of collagenous fibers in natural skin extracellular matrix can be achieved; and the composite nanofiber film has good application prospect in skin wound surface dressings.

Description

technical field [0001] The invention belongs to the field of biomedical composite materials, and in particular relates to a composite nanofibrous membrane with a bionic skin extracellular matrix structure and a preparation method and application thereof. Background technique [0002] Human skin tissue is located on the body surface and is extremely vulnerable to damage when exposed, such as burns, mechanical or chemical damage, skin ulcers, etc., resulting in partial or complete loss of skin function; at the same time, with the aging of the social age structure, various The number of chronic basic diseases and surgeries is increasing. As an important complication of diabetes and a concomitant disease highly related to population aging, chronic and difficult-to-heal wounds are becoming a major threat to the life and health of the people and bringing a heavy social medical burden. Therefore, it is of great significance to develop a wound dressing with good skin tissue repair a...

Claims

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Application Information

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IPC IPC(8): D04H1/74D01D5/00A61L15/26A61L15/32A61L15/42
Inventor 陈晓峰孙璐瑶付晓玲高文东
Owner SOUTH CHINA UNIV OF TECH
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