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Reagent plate for automatic pretreatment of nucleic acid detection

A reagent plate and nucleic acid sequencing technology, applied in the direction of biomass post-treatment, biomass pre-treatment, bioreactor/fermenter for specific purposes, etc., can solve the problems of unattended, sample impact, etc., to eliminate samples Effects of confusion, avoiding tediousness, and speeding up testing

Inactive Publication Date: 2017-02-15
HANGZHOU GENE META MEDICAL DEVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the liquid pipetting workstation is an open platform, during the whole multi-step test operation, the operator needs to seal and open the 96-well plate film, or open and close a large number of tube caps from time to time, although part of the work is done by the workstation. Completed, but the overall still requires a lot of manual intervention, and it is impossible to realize the unattended operation of the whole process, and multiple steps need manual marking, sorting, and setting
At the same time, once the test starts, it is impossible to temporarily increase or change the test process. Once there are samples that need to be processed temporarily, or the test process is changed, it will have an impact on the samples participating in the test as a whole.

Method used

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  • Reagent plate for automatic pretreatment of nucleic acid detection
  • Reagent plate for automatic pretreatment of nucleic acid detection
  • Reagent plate for automatic pretreatment of nucleic acid detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Such as figure 1 The nucleic acid detection pre-processing automation device shown is composed of a moving rail platform 1 , a base 2 , a movable magnet 3 , a pipette pump 4 and a pipette tip 5 . The pipette tip 5 is arranged on the pipette pump 4, and the pipette pump can move along the moving rail platform 1 and rotate through the rotating module. The mobile rail platform 1 moves and rotates on the upper part of the reagent plate through the pipette pump and the pipette tip to realize pipetting. The base 2 has a temperature control module corresponding to the position of the reagent disc. The base 2 is attached to the reagent tray to provide temperature control. The reagent disc can be rotated and moved by the rotating module on the base 2 .

[0042] Such as Figure 2-3 The reagent tray shown includes a cover plate 6 and a reagent tray 7. The reagent tray 7 is provided with a number of reagent holes 8, the reagent holes 8 are filled with pretreatment reagents for ...

Embodiment 2

[0045] Plasma free DNA is small fragments, fragmented DNA, the fragment length is between 100-300bp, and the peak value is around 160bp. In 1997, Professor Lu Yuming discovered that the peripheral plasma of pregnant women contained fetal cell-free DNA, and thus pioneered the use of cell-free DNA as the source of detection to analyze whether the fetus of a pregnant woman has chromosomal abnormalities. Circulating tumor DNA (ctDNA) derived from tumor cells also exists in plasma. ctDNA is widely used for tumor liquid biopsy, which can be used for non-invasive early diagnosis, companion diagnosis, and prognosis follow-up.

[0046] The conventional detection method is to first extract DNA from clinical samples, including plasma, to perform nucleic acid detection, processing, and detection in three steps. It takes 6 to 13 hours to complete the entire sample test. Among them, the extraction of sample nucleic acid and the pre-treatment process of detection are cumbersome.

[0047] A...

Embodiment 3

[0064] The research object of transcriptome sequencing is the sum of all RNAs that can be transcribed by a specific cell in a certain functional state, mainly including mRNA and non-coding RNA. Transcriptome research is the basis and starting point for the study of gene function and structure. Through next-generation high-throughput sequencing, it is possible to comprehensively and quickly obtain almost all transcript sequence information of a specific tissue or organ in a certain state, which has been widely used In basic research, clinical diagnosis and drug development and other fields.

[0065] The conventional detection method is to first extract RNA from clinical samples including whole blood and plasma, and then perform nucleic acid detection processing and detection in three steps. It takes 6 to 30 hours to complete the entire sample test. Among them, the extraction of sample nucleic acid and the pre-treatment process of detection are cumbersome.

[0066] Adopting th...

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Abstract

The invention discloses a reagent plate for automatic pretreatment of nucleic acid detection. The reagent plate is characterized by comprising a cover plate and a reagent tray, wherein a plurality of reagent holes are formed in the reagent tray; nucleic acid sequencing pretreatment reagents are contained in the reagent holes; aluminum foil sealing films are arranged at the opening of the reagent holes. The reagent plate for automatic pretreatment of nucleic acid detection, provided by the invention, in combination with an automatic pretreatment device for nucleic acid detection, can realize the full automatic pretreatment of the nucleic acid detection, including extraction, library construction and product purification; the final products can be directly applied to high-throughput sequencing; the whole process is unmanned.

Description

technical field [0001] The invention belongs to the field of nucleic acid molecular detection, and in particular relates to a reagent disk for automatic pretreatment of nucleic acid detection. The present invention realizes fully automatic nucleic acid detection pre-processing, that is, automatically realizes pre-processing such as nucleic acid extraction, amplification, and purification, and the processed samples can be detected by an external matching sequencer. The present invention simplifies the test process, reduces the chance of error, and improves The stability of the overall detection is improved. Background technique [0002] High-throughput sequencing technology is a new molecular detection technology developed in the last decade. The mainstream high-throughput sequencers on the market are mainly provided by Illumina, ThermoFisher, etc. Any high-throughput sequencing platform will first require DNA or RNA to be extracted from biological samples before testing, an...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12Q1/6806C12Q2523/308C12Q2531/113C12Q2535/122
Inventor 叶宝春黄飞祝云英
Owner HANGZHOU GENE META MEDICAL DEVICE CO LTD
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