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3D-printed artificial skin and preparation method

A technology of artificial skin and 3D printing, which is applied in the field of 3D printing artificial skin and its preparation, can solve the problems of unable to meet the needs of restoring the physiological functions of regenerative skin, and achieve the effect of being conducive to colonization and differentiation, convenient to transportation and exchange, and easy to obtain

Active Publication Date: 2017-02-15
ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Relying solely on morphological repair cannot meet the physiological function requirements of regenerated skin

Method used

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  • 3D-printed artificial skin and preparation method
  • 3D-printed artificial skin and preparation method
  • 3D-printed artificial skin and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Preparation of the braided layer: take the PLA braided wire and weave it using the Hercules braiding process to obtain a braided layer with a thickness of 200 μm and a porosity of 25%;

[0035] 2) Atmospheric pressure plasma treatment: the gas temperature used is 90°C, the flow rate is 4L / min, and the treatment time is 4min;

[0036] 3) Configure the hydrogel solution: take sodium hyaluronate and deionized water according to the molar ratio of 1:50 to configure the hydrogel solution, and then mix the epidermal growth factor, nano-silver ions and the hydrogel solution according to the ratio of 10:1: The molar ratio of 3000 is evenly mixed;

[0037] 4) Preparation of cell suspension: suspend keratinocytes and hair follicle cells in the hydrogel solution at a concentration of 2×104 / mL;

[0038] 5) Keratinocyte printing: Under sterile conditions, the keratinocyte hydrogel solution was continuously 3D printed, the spray speed of the nozzle was 10μL / s, and the moving spee...

Embodiment 2

[0042]1) Preparation of the braided layer: take the PGA braided wire and weave it using the Hercules braiding process to obtain a braided layer with a thickness of 100 μm and a porosity of 35%;

[0043] 2) Atmospheric pressure plasma treatment: the temperature of the gas used is 100°C, the flow rate is 6L / min, and the treatment time is 6min;

[0044] 3) Configure hydrogel solution: take sodium hyaluronate and deionized water according to the molar ratio of 1:30 to configure hydrogel solution, and then mix fibroblast growth factor, nano-silver ions and hydrogel material according to 10:1 : The molar ratio of 4000 is evenly mixed;

[0045] 4) Preparation of cell suspension: separate keratinocytes and hair follicle cells at 1×10 5 / mL concentration suspended in the hydrogel solution;

[0046] 5) Keratinocyte printing: Under sterile conditions, the keratinocyte hydrogel solution was continuously 3D printed, the spray speed of the nozzle was 20 μL / s, and the moving speed was 1 mm...

Embodiment 3

[0050] 1) Preparation of braided layer: take PLGA braided wire and adopt two-dimensional triaxial braiding process to weave to obtain a braided layer with a thickness of 150 μm and a porosity of 20%;

[0051] 2) Atmospheric pressure plasma treatment: the temperature of the gas used is 100°C, the flow rate is 8L / min, and the treatment time is 8min;

[0052] 3) Configure the hydrogel solution: take sodium hyaluronate and deionized water according to the molar ratio of 1:40 to configure the hydrogel solution, and then mix the vascular endothelial cell growth factor, nano-silver ions and the hydrogel material according to the ratio of 10: The molar ratio of 1:5000 is evenly mixed;

[0053] 4) Preparation of cell suspension: separate keratinocytes and hair follicle cells at 2.5×10 6 / mL concentration suspended in the hydrogel solution;

[0054] 5) Keratinocyte printing: Under sterile conditions, the keratinocyte hydrogel solution was continuously 3D printed, the nozzle spray spee...

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Abstract

The invention relates to 3D-printed artificial skin. The 3D-printed artificial skin comprises a keratinocyte containing hydrogel layer, a woven layer, a hair follicle cell containing hydrogel layer and another woven layer in sequence from top to bottom; the woven layers are formed by weaving macromolecule degradable materials and contain pores; the keratinocyte containing hydrogel layer and the hair follicle cell containing hydrogel layer both contain epidermal growth factors and nano-silver. The 3D-printed artificial skin has the advantages that the adopted materials are macromolecule degradable materials which are easy to obtain and good in biocompatibility, and the woven layers obtained through weaving have many pores which are beneficial for transport and exchange of nutrient substances; due to a multi-layer structure, the wear resistance, toughness, mechanical strength and other physical properties of the artificial skin are enhanced; through normal-pressure plasma jet treatment, the superficial areas of the woven layers are increased, and adhesion between hydrogel and skin appendage cells is promoted; the artificial skin primarily has a normal skin structure after being manufactured, and recovery of skin functions is convenient.

Description

technical field [0001] The invention belongs to the field of tissue engineering biomedical materials, and in particular relates to 3D printing artificial skin and a preparation method. Background technique [0002] As the largest organ in the human body, the skin is a natural barrier between the human body and the external environment, and has the functions of protection, regulation, and excretion. When skin defects and abnormalities are caused by inflammation, ulcers, trauma, burns, surgery, etc., it will affect the appearance of the patient in the slightest, and cause infection or a large loss of electrolytes and water in severe cases, resulting in the death of the patient. Especially for the treatment of large-area skin defects, the key is to seal the wound as soon as possible, reduce the consumption of the body and the disorder of the internal environment, prevent the invasion of harmful substances, and promote the regeneration of the wound skin. At present, autologous ...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/38A61L27/20A61L27/18A61L27/50A61L27/56A61L27/52A61L27/54A61L27/60
CPCA61L27/18A61L27/20A61L27/3891A61L27/50A61L27/52A61L27/54A61L27/56A61L27/60A61L2300/104A61L2300/404A61L2300/414C08L5/08C08L67/04
Inventor 周佳厉民王峥
Owner ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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