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A kind of preparation method of composite artificial dura mater

An artificial dura mater, composite technology, applied in the field of biomedical materials, can solve the problems of scarring or wrapping reaction in the retention time, increasing the time of surgery and treatment, weak biocompatibility, etc. Industrial preparation, the effect of increasing fiber surface area

Active Publication Date: 2019-08-02
ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although autologous tissue repair materials have good biocompatibility and low immune response, their sources are limited, which can easily cause new defects and increase the time of surgery and treatment
Allogeneic and xenogeneic biological materials are readily available, but there is a risk of immune rejection
At present, artificial synthetic materials are mostly made of polymer materials through one-time molding. The hydrophilicity is not ideal, and the biocompatibility is relatively weak. After a long time of storage, it will form scars or wrap reactions, which can cause stimulation to brain tissue, and can cause local fluid accumulation or Complications such as aseptic inflammation

Method used

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  • A kind of preparation method of composite artificial dura mater
  • A kind of preparation method of composite artificial dura mater
  • A kind of preparation method of composite artificial dura mater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) select fibrin and deionized water to configure the hydrogel solution according to the molar ratio of 1:10;

[0030] 2) making the fibroblast growth factor and the hydrogel material into a suspension according to the molar ratio of 1:5000;

[0031] 3) Place the hydrogel suspension in a film-forming mold to form a film with a thickness of 100 μm, and cure at 4°C for 2 hours;

[0032] 4) The braided wire is made of PLA, and the surface is treated with helium plasma, the gas temperature is 90°C, the flow rate is 4L / min, and the treatment time is 4min;

[0033] 5) The braided wire is made of the Heglis braiding process to make the braided layer with a thickness of 500 μm;

[0034] 6) After the braided layer is laid on the cured hydrogel, a layer of hydrogel suspension with a thickness of 100 μm is laid on the braided layer, and cured at 4°C for 2 hours;

[0035] 7) The artificial dura mater is sterilized with a radiation dose of 25kGy.

Embodiment 2

[0037] 1) Select collagen and deionized water to configure the hydrogel solution according to the molar ratio of 1:20;

[0038] 2) making the fibroblast growth factor and the hydrogel material into a suspension according to the molar ratio of 1:4000;

[0039] 3) Place the hydrogel suspension in a film-forming mold to form a film with a thickness of 200 μm, and cure at 4°C for 3 hours;

[0040] 4) The braided wire is made of PGA, and the surface is treated with helium plasma, the gas temperature is 100°C, the flow rate is 6L / min, and the treatment time is 6min;

[0041] 5) The braided wire is made of the Heglis braiding process to make the braided layer with a thickness of 1000 μm;

[0042] 6) After the braided layer is laid on the cured hydrogel, a layer of hydrogel suspension with a thickness of 200 μm is laid on the braided layer, and cured at 4°C for 3 hours;

[0043] 7) The artificial dura mater is sterilized by giving the irradiation dose of 28kGy.

Embodiment 3

[0045] 1) select collagen and deionized water to configure hydrogel solution according to the molar ratio of 1:20;

[0046] 2) The vascular endothelial growth factor and the hydrogel material are made into a suspension according to the molar ratio of 1:4000;

[0047] 3) Place the hydrogel suspension in a film-forming mold to form a film with a thickness of 300 μm, and cure at 6°C for 3 hours;

[0048] 4) The braided wire is made of PLGA, and the surface is treated with oxygen plasma, the gas temperature is 100°C, the flow rate is 8L / min, and the treatment time is 8min;

[0049] 5) The braided wire is made of a two-dimensional three-axis braiding process to make a braided layer with a thickness of 1000 μm;

[0050] 6) After the braided layer is laid on the cured hydrogel, a layer of hydrogel suspension with a thickness of 300 μm is laid on the braided layer, and cured at 6°C for 3 hours;

[0051] 7) The artificial dura mater is sterilized by giving the irradiation dose of 30k...

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Abstract

The invention relates to a composite type artificial dura mater, comprising two hydrogel layers and a woven layer, wherein the woven layer is arranged between the two hydrogel layers, the woven layer is a degradable polymer material, and cell growth factors are contained in the hydrogel layers. The composite type artificial dura mater disclosed by the invention has the following beneficial effects: (1) the artificial dura mater is made from the degradable polymer material and biological protein which are easily available, and has good biocompatibility; (2) atmospheric pressure plasma jet treatment increases the fiber surface area of the woven layer, decreases a contact angle of a material surface, and improves water transmissibility, degradation speed and cellular affinity; (3) regulation on physical characteristics of the artificial dura mater, such as malleability, toughness, mechanical strength and the like can be realized by regulating a weaving method; and 4) the composite type artificial dura mater is favorable for wound repair, and silver ions enable artificial skin to have good antibacterial performance, so that toxic and side effects on a human body due to the use of a chemical antibacterial medicine are avoided, and storage life of the artificial skin is prolonged.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a preparation method of a composite artificial dura mater. Background technique [0002] The dura mater is a relatively strong connective tissue film. The natural dura mater structure is mainly composed of fibroblasts and collagen fibers. It is located in the outermost layer of the brain and spinal cord structures. It is an important barrier to protect brain tissue. The structure and functional activities of the system are of great significance. Trauma or craniocerebral surgery can cause dura mater defect, if not repaired, it is easy to lead to non-healing of the wound, and complications such as subcutaneous fluid accumulation, cerebrospinal fluid leakage, and intracranial infection may occur; oozing blood from the scalp or muscle layer can diffuse into the arachnoid membrane The inferior space stimulates the brain tissue, causing fever, headache, aseptic inflammation or meni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61L27/40A61L27/24A61L27/22A61L27/18A61L27/52A61L27/54
CPCA61L27/18A61L27/225A61L27/24A61L27/52A61L27/54A61L2300/414C08L89/00C08L67/04
Inventor 周佳厉民王峥
Owner ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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