Biological active padding material of fermentation bed for poultry, preparation method and application
A technology of biological activity and fermentation bed, which is applied in the preparation of composite microbial active litter and the field of composite microbial active litter, can solve the cost of litter that cannot be effectively inactivated by pathogenic microorganisms, low efficiency of excreta treatment, secondary pollution, etc. problems, to achieve the effect of reducing breeding costs, reducing the probability of infecting animals and people, and reducing costs
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Embodiment 1
[0047] Fermentation bed bioactive bedding for poultry is made of the following raw materials in weight percentage: 24% corn stalks, 35% sawdust, 6% rice bran, 10% bran, 3% superphosphate, 20% chicken manure, fermentation bacteria Agent 2%.
[0048] Preparation of fermented inoculum, the steps are:
[0049] 1) Activation and cultivation of Bacillus cereus strains: the medium is ordinary nutrient agar medium, and the nutrient composition of the medium is 20 grams of peptone per 1 L, 5 grams of beef powder, and 5 grams of sodium chloride, and the pH is adjusted to 7.2± 0.2, the inoculation amount accounts for 0.5% of the culture medium, the temperature is 35°C, and the culture time is 48 hours; the culture end point is that the number of Bacillus cereus is not less than 25% of the total number of bacteria, and the number of viable bacteria in the culture medium is 1.0×10 9 ~1×10 10 cfu / mL;
[0050] 2) Activation and cultivation of Bacillus licheniformis strains: the medium is ...
Embodiment 2
[0058] Fermentation bed bioactive litter for poultry, made of the following raw materials by weight percentage: 24% cotton straw, 35% sawdust, 6% wheat bran, 10% cotton seed hulls, 3% sodium superphosphate, 20% chicken manure, fermented Bacteria agent 2%.
[0059] Preparation of fermented inoculum, the steps are:
[0060] 1) Activation and cultivation of Bacillus cereus strains: the medium is ordinary nutrient agar medium, and the nutrient composition of the medium is 20 grams of peptone per 1 L, 5 grams of beef powder, and 5 grams of sodium chloride, and the pH is adjusted to 7.2± 0.2. The inoculation amount accounts for 1% of the culture medium, the temperature is 45°C, and the culture time is 48 hours; the culture end point is that the number of Bacillus cereus is not less than 25% of the total number of bacteria, and the number of viable bacteria in the culture medium is 1.0×10 9 ~1×10 10 cfu / mL;
[0061] 2) Activation and cultivation of Bacillus licheniformis strains:...
Embodiment 3
[0070] Fermentation bed bioactive litter for poultry, made of the following raw materials by weight percentage: rice husk 24%, sawdust 35%, distiller's grains 6%, bran 10%, superphosphate 3%, chicken manure 20%, fermentation bacteria 2%.
[0071] Preparation of fermented inoculum, the steps are:
[0072] 1) Activation and cultivation of Bacillus cereus strains: the medium is ordinary nutrient agar medium, and the nutrient composition of the medium is 20 grams of peptone per 1 L, 5 grams of beef powder, and 5 grams of sodium chloride, and the pH is adjusted to 7.2± 0.2, the inoculation amount accounts for 0.8% of the culture medium, the temperature is 20°C, and the culture time is 48 hours; the culture end point is that the number of Bacillus cereus is not less than 25% of the total number of bacteria, and the number of viable bacteria in the culture medium is 1.0×10 9 ~1×10 10 cfu / mL;
[0073] 2) Activation and cultivation of Bacillus licheniformis strains: the medium is o...
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