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Molecular marker related to prostate cancer and applications of molecular marker

A technology of molecular markers and prostate cancer, applied in the biological field, can solve problems such as misdiagnosis of disease, misdiagnosis of disease, failure to reach early diagnosis, etc.

Inactive Publication Date: 2017-01-04
北京致成生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, the PSA in the blood is not high (not higher than 4ng / ml). When in the state of prostate cancer and other prostate diseases, the increase of PSA has become the most sensitive tumor marker for screening prostate cancer, but the increase of PSA is also It is often related to non-prostate cancer diseases, such as prostatitis, benign prostatic hyperplasia and other urinary system diseases, so it is not easy to diagnose, and may even lead to misdiagnosis, misdiagnosis or delay of disease
In addition, when prostate cancer is diagnosed by an increase in PSA, the patient is often already in the middle and late stages, and the purpose of early diagnosis cannot be achieved.
However, the detection rate of needle biopsy is closely related to the number and location of biopsy, which is not a very stable diagnostic solution.

Method used

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  • Molecular marker related to prostate cancer and applications of molecular marker
  • Molecular marker related to prostate cancer and applications of molecular marker
  • Molecular marker related to prostate cancer and applications of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 High-throughput sequencing screening of differentially expressed genes

[0035] 1. Sampling

[0036]Select 10 cases of fresh prostate cancer tissues and corresponding paracancerous tissues, samples from prostate cancer patients who underwent radical prostatectomy and bilateral pelvic lymph node dissection from October 2012 to December 2015 in Peking Union Medical College Hospital. Under the guidance of the pathologist, the prostate cancer and paracancerous tissues were immediately taken and put into liquid nitrogen, and stored in a -80°C low-temperature refrigerator after serial numbering.

[0037] 2. Total RNA extraction from tissue samples

[0038] use Reagent (invitrogen, Cat. No. 15596-018) was used to extract sample RNA, and the experimental operation was carried out according to the product manual. The specific operation was as follows:

[0039] Collect the samples and freeze them in liquid nitrogen. After taking them out, put the tissue samples into...

Embodiment 2

[0050] Example 2 RT-PCR verification of the expression of LOC100190940 in prostate cancer tissue

[0051] 1. Materials

[0052] 10 prostate cancer patients were selected, and the samples were collected from the prostate cancer patients who underwent radical prostatectomy and bilateral pelvic lymph node dissection from October 2012 to December 2015 in Peking Union Medical College Hospital. Immediately take prostate cancer and paracancerous tissues and put them into liquid nitrogen, and store them in a -80°C low-temperature refrigerator after serial numbering.

[0053] 2. Method

[0054] 2.1 Extract the total RNA from the tissue samples of the subjects, the same as the extraction method in Example 1.

[0055] 2.2 Synthesis of cDNA by reverse transcription

[0056] use III Reverse Transcriptase (invitrogen, Cat. No. 18080-044) was used for cDNA reverse transcription, and the experimental operation was carried out according to the product manual. The specific operation was as...

Embodiment 3

[0076] The preparation of embodiment 3 kits

[0077] Based on the primer set obtained in Example 2, assemble the test kit for detecting prostate cancer according to the present invention, the kit includes a primer pair for specific amplification of LOC100190940 as shown in SEQ ID NO:2 and SEQ ID NO:3 , and the primer pair for specifically amplifying the housekeeping gene (GAPDH) are shown in SEQ ID NO:4 and SEQ ID NO:5; also include SYBR Green polymerase chain reaction system, such as PCR buffer, SYBR Green fluorescent dye, dNTPs . The composition of described PCR buffer is 25mMKCl, 2.5mM MgCl 2 , 200mM (NH 4 ) 2 SO 4 ; Also includes normal prostate tissue cDNA: used as a negative control and detection sample cDNA for quantitative PCR detection, each reaction system uses the same amount as the detection sample cDNA.

[0078] Through the optimization of primer concentration and annealing temperature, the reaction system was finally determined as shown in Table 3:

[0079]...

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Abstract

The invention discloses a molecular marker related to prostate cancer. The molecular marker is LOC100190940. The invention also discloses a diagnostic kit for the prostate cancer. The prostate cancer contains the molecular marker LOC100190940 related to the prostate cancer. The invention further discloses applications of the molecular marker in screening, diagnosis, treatment, monitoring and prognosis of prostate cancer high-risk populations. With the application of the molecular marker and the diagnostic kit containing the molecular marker in detection of the prostate cancer, early detection can be realized rapidly and effectively, and a treatment target and an important basis are provided for clinical applications such as gene therapy, drug therapy and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker related to prostate cancer and its application. Background technique [0002] Prostate cancer is a malignant tumor that occurs in the prostate tissue of men. It has become the second most common tumor in men worldwide and ranks sixth in cancer-related mortality. In developed countries and regions such as Europe and the United States, it is the most common malignant tumor in men, and its mortality rate ranks second among various cancers; in Asia, its incidence rate is lower than that of Western countries, but it has shown a rapid upward trend in recent years faster than developed countries in Europe and America. The incidence of prostate cancer in my country is also increasing year by year, ranking third among male genitourinary system malignancies, second only to bladder cancer and kidney cancer. Prostate cancer generally occurs after the age of 50, and 95% of it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/113A61K45/00A61P35/00
CPCC12Q1/6886A61K45/00C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 刘昊
Owner 北京致成生物医学科技有限公司
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